Proteomic profile of human monocytic cells infected with dengue virus
Objective: To identify the changes in the proteome of U937 cells infected with dengue virus (DENV). Methods: In this study, differentiated U937 cultures were infected with two DENV-2 strains, one of which was associated with dengue (DENV-2/NG) and the other one with severe dengue (DENV-2/16681), wit...
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doaj-e4dd1e9fbc6f4ad6ad41f72e494ee0af2020-11-24T22:32:04ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912016-11-0161191492310.1016/j.apjtb.2016.01.004Proteomic profile of human monocytic cells infected with dengue virusViviana Martínez-Betancur0Marlén Martínez-Gutierrez1Programa de Estudio y Control de Enfermedades Tropicales-PECET, Universidad de Antioquia, Medellín, ColombiaPrograma de Estudio y Control de Enfermedades Tropicales-PECET, Universidad de Antioquia, Medellín, ColombiaObjective: To identify the changes in the proteome of U937 cells infected with dengue virus (DENV). Methods: In this study, differentiated U937 cultures were infected with two DENV-2 strains, one of which was associated with dengue (DENV-2/NG) and the other one with severe dengue (DENV-2/16681), with the aim of determining the cellular proteomic profiles under different infection conditions. Cellular proteins were extracted and separated by two-dimensional electrophoresis, and those proteins with differential expression profiles were identified by mass spectrometry. The obtained results were correlated with cellular viability, the number of infectious viral particles, and the viral DNA/protein quantity. Results: In comparison with non-infected cultures, in the cells infected with the DENV-2/NG strain, nine proteins were expressed differentially (five were upregulated and four were downregulated); in those cultures infected with the DENV-2/16681 strain, six proteins were differentially expressed (two were downregulated and four were upregulated). The downregulated proteins included fatty acid-binding protein, heterogeneous nuclear ribonucleoprotein 1, protein disulfide isomerase, enolase 1, heat shock 70 kDa protein 9, phosphotyrosyl phosphatase, and annexin IV. The upregulated proteins included heat shock 90 kDa protein AA1, tubulin beta, enolase 1, pyruvate kinase, transaldolase and phospholipase C-alpha. Conclusions: Because the monocyte/macrophage lineage is critical for disease pathogenicity, additional studies on these proteins could provide a better understanding of the cellular response to DENV infection and could help identify new therapeutic targets against infection.http://www.sciencedirect.com/science/article/pii/S2221169115307917Dengue virusProteomicMacrophagesMonocytesU937 cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Viviana Martínez-Betancur Marlén Martínez-Gutierrez |
spellingShingle |
Viviana Martínez-Betancur Marlén Martínez-Gutierrez Proteomic profile of human monocytic cells infected with dengue virus Asian Pacific Journal of Tropical Biomedicine Dengue virus Proteomic Macrophages Monocytes U937 cells |
author_facet |
Viviana Martínez-Betancur Marlén Martínez-Gutierrez |
author_sort |
Viviana Martínez-Betancur |
title |
Proteomic profile of human monocytic cells infected with dengue virus |
title_short |
Proteomic profile of human monocytic cells infected with dengue virus |
title_full |
Proteomic profile of human monocytic cells infected with dengue virus |
title_fullStr |
Proteomic profile of human monocytic cells infected with dengue virus |
title_full_unstemmed |
Proteomic profile of human monocytic cells infected with dengue virus |
title_sort |
proteomic profile of human monocytic cells infected with dengue virus |
publisher |
Wolters Kluwer Medknow Publications |
series |
Asian Pacific Journal of Tropical Biomedicine |
issn |
2221-1691 |
publishDate |
2016-11-01 |
description |
Objective: To identify the changes in the proteome of U937 cells infected with dengue virus (DENV).
Methods: In this study, differentiated U937 cultures were infected with two DENV-2 strains, one of which was associated with dengue (DENV-2/NG) and the other one with severe dengue (DENV-2/16681), with the aim of determining the cellular proteomic profiles under different infection conditions. Cellular proteins were extracted and separated by two-dimensional electrophoresis, and those proteins with differential expression profiles were identified by mass spectrometry. The obtained results were correlated with cellular viability, the number of infectious viral particles, and the viral DNA/protein quantity.
Results: In comparison with non-infected cultures, in the cells infected with the DENV-2/NG strain, nine proteins were expressed differentially (five were upregulated and four were downregulated); in those cultures infected with the DENV-2/16681 strain, six proteins were differentially expressed (two were downregulated and four were upregulated). The downregulated proteins included fatty acid-binding protein, heterogeneous nuclear ribonucleoprotein 1, protein disulfide isomerase, enolase 1, heat shock 70 kDa protein 9, phosphotyrosyl phosphatase, and annexin IV. The upregulated proteins included heat shock 90 kDa protein AA1, tubulin beta, enolase 1, pyruvate kinase, transaldolase and phospholipase C-alpha.
Conclusions: Because the monocyte/macrophage lineage is critical for disease pathogenicity, additional studies on these proteins could provide a better understanding of the cellular response to DENV infection and could help identify new therapeutic targets against infection. |
topic |
Dengue virus Proteomic Macrophages Monocytes U937 cells |
url |
http://www.sciencedirect.com/science/article/pii/S2221169115307917 |
work_keys_str_mv |
AT vivianamartinezbetancur proteomicprofileofhumanmonocyticcellsinfectedwithdenguevirus AT marlenmartinezgutierrez proteomicprofileofhumanmonocyticcellsinfectedwithdenguevirus |
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