Gene Organization, Expression, and Localization of Ribotoxin-Like Protein Ageritin in Fruiting Body and Mycelium of <i>Agrocybe aegerita</i>

• Main Authors: Ilaria Baglivo, Sara Ragucci, Paolo D’Incecco, Nicola Landi, Rosita Russo, Franco Faoro, Paolo V. Pedone, Antimo Di Maro
• Format: Article
• Language: English
• Published: MDPI AG 2020-09-01
• Series: International Journal of Molecular Sciences
• Subjects: <i>Agrocybe aegerita</i>; cDNA extraction; immunolocalization; signal peptide; ribotoxin-like proteins; protein routing
• Online Access: https://www.mdpi.com/1422-0067/21/19/7158
• ISSN: 1661-6596; 1422-0067

The edible mushroom <i>Agrocybe aegerita</i> produces a ribotoxin-like protein known as Ageritin. In this work, the gene encoding Ageritin was characterized by sequence analysis. It contains several typical features of fungal genes such as three short introns (60, 55 and 69 bp) located at the 5′ region of the coding sequence and typical splice junctions. This sequence codes for a precursor of 156 amino acids (~17-kDa) containing an additional <i>N</i>-terminal peptide of 21 amino acid residues, absent in the purified toxin (135 amino acid residues; ~15-kDa). The presence of 17-kDa and 15-kDa forms was investigated by Western blot in specific parts of fruiting body and in mycelia of <i>A. aegerita</i>. Data show that the 15-kDa Ageritin is the only form retrieved in the fruiting body and the principal form in mycelium. The immunolocalization by confocal laser scanning microscopy and transmission electron microscopy proves that Ageritin has vacuolar localization in hyphae. Coupling these data with a bioinformatics approach, we suggest that the <i>N</i>-terminal peptide of Ageritin (not found in the purified toxin) is a new signal peptide in fungi involved in intracellular routing from endoplasmic reticulum to vacuole, necessary for self-defense of <i>A. aegerita</i> ribosomes from Ageritin toxicity.