Upregulated Chemokine and Rho-GTPase Genes Define Immune Cell Emigration into Salivary Glands of Sjögren’s Syndrome-Susceptible C57BL/6.NOD-<i>Aec1Aec2</i> Mice
The C57BL/6.NOD-<i>Aec1Aec2</i> mouse is considered a highly appropriate model of Sjögren’s Syndrome (SS), a human systemic autoimmune disease characterized primarily as the loss of lacrimal and salivary gland functions. This mouse model, as well as other mouse models of SS, have shown t...
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doaj-e4e335c4be004c40b7748a9d063a6dde2021-07-15T15:38:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-01227176717610.3390/ijms22137176Upregulated Chemokine and Rho-GTPase Genes Define Immune Cell Emigration into Salivary Glands of Sjögren’s Syndrome-Susceptible C57BL/6.NOD-<i>Aec1Aec2</i> MiceAmmon B. Peck0Cuong Q. Nguyen1Julian L. Ambrus2Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, P.O. Box 100125, Gainesville, FL 32610, USADepartment of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, P.O. Box 100125, Gainesville, FL 32610, USADivision of Allergy, Immunology and Rheumatology, SUNY Buffalo School of Medicine, 875 Ellicott Street, Buffalo, NY 14203, USAThe C57BL/6.NOD-<i>Aec1Aec2</i> mouse is considered a highly appropriate model of Sjögren’s Syndrome (SS), a human systemic autoimmune disease characterized primarily as the loss of lacrimal and salivary gland functions. This mouse model, as well as other mouse models of SS, have shown that B lymphocytes are essential for the development and onset of observed clinical manifestations. More recently, studies carried out in the C57BL/6.<i>IL14α</i> transgenic mouse have indicated that the marginal zone B (MZB) cell population is responsible for development of SS disease, reflecting recent observations that MZB cells are present in the salivary glands of SS patients and most likely initiate the subsequent loss of exocrine functions. Although MZB cells are difficult to study in vivo and in vitro, we have carried out an <i>ex vivo</i> investigation that uses temporal global RNA transcriptomic analyses to profile differentially expressed genes known to be associated with cell migration. Results indicate a temporal upregulation of specific chemokine, chemokine receptor, and Rho-GTPase genes in the salivary glands of C57BL/6.NOD-<i>Aec1Aec2</i> mice that correlate with the early appearance of periductal lymphocyte infiltrations. Using the power of transcriptomic analyses to better define the genetic profile of lymphocytic emigration into the salivary glands of SS mice, new insights into the underlying mechanisms of SS disease development and onset begin to come into focus, thereby establishing a foundation for further in-depth and novel investigations of the covert and early overt phases of SS disease at the cellular level.https://www.mdpi.com/1422-0067/22/13/7176Sjögren’s syndromemarginal zone B cellsRNA transcriptome microarrayRho-GTPasesGTP-GAPGTP-GEF |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ammon B. Peck Cuong Q. Nguyen Julian L. Ambrus |
spellingShingle |
Ammon B. Peck Cuong Q. Nguyen Julian L. Ambrus Upregulated Chemokine and Rho-GTPase Genes Define Immune Cell Emigration into Salivary Glands of Sjögren’s Syndrome-Susceptible C57BL/6.NOD-<i>Aec1Aec2</i> Mice International Journal of Molecular Sciences Sjögren’s syndrome marginal zone B cells RNA transcriptome microarray Rho-GTPases GTP-GAP GTP-GEF |
author_facet |
Ammon B. Peck Cuong Q. Nguyen Julian L. Ambrus |
author_sort |
Ammon B. Peck |
title |
Upregulated Chemokine and Rho-GTPase Genes Define Immune Cell Emigration into Salivary Glands of Sjögren’s Syndrome-Susceptible C57BL/6.NOD-<i>Aec1Aec2</i> Mice |
title_short |
Upregulated Chemokine and Rho-GTPase Genes Define Immune Cell Emigration into Salivary Glands of Sjögren’s Syndrome-Susceptible C57BL/6.NOD-<i>Aec1Aec2</i> Mice |
title_full |
Upregulated Chemokine and Rho-GTPase Genes Define Immune Cell Emigration into Salivary Glands of Sjögren’s Syndrome-Susceptible C57BL/6.NOD-<i>Aec1Aec2</i> Mice |
title_fullStr |
Upregulated Chemokine and Rho-GTPase Genes Define Immune Cell Emigration into Salivary Glands of Sjögren’s Syndrome-Susceptible C57BL/6.NOD-<i>Aec1Aec2</i> Mice |
title_full_unstemmed |
Upregulated Chemokine and Rho-GTPase Genes Define Immune Cell Emigration into Salivary Glands of Sjögren’s Syndrome-Susceptible C57BL/6.NOD-<i>Aec1Aec2</i> Mice |
title_sort |
upregulated chemokine and rho-gtpase genes define immune cell emigration into salivary glands of sjögren’s syndrome-susceptible c57bl/6.nod-<i>aec1aec2</i> mice |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-07-01 |
description |
The C57BL/6.NOD-<i>Aec1Aec2</i> mouse is considered a highly appropriate model of Sjögren’s Syndrome (SS), a human systemic autoimmune disease characterized primarily as the loss of lacrimal and salivary gland functions. This mouse model, as well as other mouse models of SS, have shown that B lymphocytes are essential for the development and onset of observed clinical manifestations. More recently, studies carried out in the C57BL/6.<i>IL14α</i> transgenic mouse have indicated that the marginal zone B (MZB) cell population is responsible for development of SS disease, reflecting recent observations that MZB cells are present in the salivary glands of SS patients and most likely initiate the subsequent loss of exocrine functions. Although MZB cells are difficult to study in vivo and in vitro, we have carried out an <i>ex vivo</i> investigation that uses temporal global RNA transcriptomic analyses to profile differentially expressed genes known to be associated with cell migration. Results indicate a temporal upregulation of specific chemokine, chemokine receptor, and Rho-GTPase genes in the salivary glands of C57BL/6.NOD-<i>Aec1Aec2</i> mice that correlate with the early appearance of periductal lymphocyte infiltrations. Using the power of transcriptomic analyses to better define the genetic profile of lymphocytic emigration into the salivary glands of SS mice, new insights into the underlying mechanisms of SS disease development and onset begin to come into focus, thereby establishing a foundation for further in-depth and novel investigations of the covert and early overt phases of SS disease at the cellular level. |
topic |
Sjögren’s syndrome marginal zone B cells RNA transcriptome microarray Rho-GTPases GTP-GAP GTP-GEF |
url |
https://www.mdpi.com/1422-0067/22/13/7176 |
work_keys_str_mv |
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