Changes in the Ultrastructure of <i>Staphylococcus aureus</i> Treated with Cationic Peptides and Chlorhexidine
Antimicrobial peptides, including synthetic ones, are becoming increasingly important as a promising tool to fight multidrug-resistant bacteria. We examined the effect of cationic peptides H<sub>2</sub>N-Arg<sub>9</sub>-Phe<sub>2</sub>-C(O)NH<sub>2</sub&g...
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doaj-e4e77d161985435592413e0fa8bc895a2020-12-15T00:02:38ZengMDPI AGMicroorganisms2076-26072020-12-0181991199110.3390/microorganisms8121991Changes in the Ultrastructure of <i>Staphylococcus aureus</i> Treated with Cationic Peptides and ChlorhexidineAlina Grigor’eva0Alevtina Bardasheva1Anastasiya Tupitsyna2Nariman Amirkhanov3Nina Tikunova4Dmitrii Pyshnyi5Elena Ryabchikova6Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Science, Lavrent’ev av. 8, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Science, Lavrent’ev av. 8, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Science, Lavrent’ev av. 8, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Science, Lavrent’ev av. 8, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Science, Lavrent’ev av. 8, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Science, Lavrent’ev av. 8, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Science, Lavrent’ev av. 8, 630090 Novosibirsk, RussiaAntimicrobial peptides, including synthetic ones, are becoming increasingly important as a promising tool to fight multidrug-resistant bacteria. We examined the effect of cationic peptides H<sub>2</sub>N-Arg<sub>9</sub>-Phe<sub>2</sub>-C(O)NH<sub>2</sub> and H<sub>2</sub>N-(Lys-Phe-Phe)<sub>3</sub>-Lys-C(O)NH<sub>2</sub> on <i>Staphylococcus aureus</i>, which remains one of the most harmful pathogens. Antiseptic chlorhexidine served as reference preparation. We studied viability of <i>S. aureus</i> and examined its ultrastructure under treatment with 100 µM of R9F2 or (KFF)3K peptides or chlorhexidine using transmission electron microscopy of ultrathin sections. Bacterial cells were sampled as kinetic series starting from 1 min up to 4 h of treatment with preparations. Both peptides caused clearly visible damage of bacteria cell membrane within 1 min. Incubation of <i>S. aureus</i> with R9F2 or (KFF)3K peptides led to cell wall thinning, loss of cytoplasm structure, formation of mesosome-derived multimembrane structures and “decorated fibers” derived from DNA chains. The effect of R9F2 peptides on <i>S. aureus</i> was more severe than the effect of (KFF)3K peptides. Chlorhexidine heavily damaged the bacteria cell wall, in particular in areas of septa formation, while cytoplasm kept its structure within the observation time. Our study showed that cell membrane damage is critical for <i>S. aureus</i> viability; however, we believe that cell wall disorders should also be taken into account when analyzing the effects of the mechanisms of action of antimicrobial peptides (AMPs).https://www.mdpi.com/2076-2607/8/12/1991<i>S. aureus</i>transmission electron microscopycationic peptideschlorhexidinecell membranecell wall |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alina Grigor’eva Alevtina Bardasheva Anastasiya Tupitsyna Nariman Amirkhanov Nina Tikunova Dmitrii Pyshnyi Elena Ryabchikova |
spellingShingle |
Alina Grigor’eva Alevtina Bardasheva Anastasiya Tupitsyna Nariman Amirkhanov Nina Tikunova Dmitrii Pyshnyi Elena Ryabchikova Changes in the Ultrastructure of <i>Staphylococcus aureus</i> Treated with Cationic Peptides and Chlorhexidine Microorganisms <i>S. aureus</i> transmission electron microscopy cationic peptides chlorhexidine cell membrane cell wall |
author_facet |
Alina Grigor’eva Alevtina Bardasheva Anastasiya Tupitsyna Nariman Amirkhanov Nina Tikunova Dmitrii Pyshnyi Elena Ryabchikova |
author_sort |
Alina Grigor’eva |
title |
Changes in the Ultrastructure of <i>Staphylococcus aureus</i> Treated with Cationic Peptides and Chlorhexidine |
title_short |
Changes in the Ultrastructure of <i>Staphylococcus aureus</i> Treated with Cationic Peptides and Chlorhexidine |
title_full |
Changes in the Ultrastructure of <i>Staphylococcus aureus</i> Treated with Cationic Peptides and Chlorhexidine |
title_fullStr |
Changes in the Ultrastructure of <i>Staphylococcus aureus</i> Treated with Cationic Peptides and Chlorhexidine |
title_full_unstemmed |
Changes in the Ultrastructure of <i>Staphylococcus aureus</i> Treated with Cationic Peptides and Chlorhexidine |
title_sort |
changes in the ultrastructure of <i>staphylococcus aureus</i> treated with cationic peptides and chlorhexidine |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2020-12-01 |
description |
Antimicrobial peptides, including synthetic ones, are becoming increasingly important as a promising tool to fight multidrug-resistant bacteria. We examined the effect of cationic peptides H<sub>2</sub>N-Arg<sub>9</sub>-Phe<sub>2</sub>-C(O)NH<sub>2</sub> and H<sub>2</sub>N-(Lys-Phe-Phe)<sub>3</sub>-Lys-C(O)NH<sub>2</sub> on <i>Staphylococcus aureus</i>, which remains one of the most harmful pathogens. Antiseptic chlorhexidine served as reference preparation. We studied viability of <i>S. aureus</i> and examined its ultrastructure under treatment with 100 µM of R9F2 or (KFF)3K peptides or chlorhexidine using transmission electron microscopy of ultrathin sections. Bacterial cells were sampled as kinetic series starting from 1 min up to 4 h of treatment with preparations. Both peptides caused clearly visible damage of bacteria cell membrane within 1 min. Incubation of <i>S. aureus</i> with R9F2 or (KFF)3K peptides led to cell wall thinning, loss of cytoplasm structure, formation of mesosome-derived multimembrane structures and “decorated fibers” derived from DNA chains. The effect of R9F2 peptides on <i>S. aureus</i> was more severe than the effect of (KFF)3K peptides. Chlorhexidine heavily damaged the bacteria cell wall, in particular in areas of septa formation, while cytoplasm kept its structure within the observation time. Our study showed that cell membrane damage is critical for <i>S. aureus</i> viability; however, we believe that cell wall disorders should also be taken into account when analyzing the effects of the mechanisms of action of antimicrobial peptides (AMPs). |
topic |
<i>S. aureus</i> transmission electron microscopy cationic peptides chlorhexidine cell membrane cell wall |
url |
https://www.mdpi.com/2076-2607/8/12/1991 |
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