Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the <i>Tetranychus urticae</i> CYP392A16-Based Abamectin Resistance in <i>Drosophila</i>
Overexpression of the cytochrome P450 monooxygenase CYP392A16 has been previously associated with abamectin resistance using transcriptional analysis in the two-spotted spider mite <i>Tetranychus urticae</i>, an important pest species worldwide; however, this association has not been fun...
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doaj-e4ed2db6d23a4585ad84811a5fb24faa2020-11-27T08:00:47ZengMDPI AGInsects2075-44502020-11-011182982910.3390/insects11120829Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the <i>Tetranychus urticae</i> CYP392A16-Based Abamectin Resistance in <i>Drosophila</i>Maria Riga0Aris Ilias1John Vontas2Vassilis Douris3Institute of Molecular Biology & Biotechnology, Foundation for Research & Technology Hellas, 100 N. Plastira Street, GR-700 13 Heraklion, GreeceInstitute of Molecular Biology & Biotechnology, Foundation for Research & Technology Hellas, 100 N. Plastira Street, GR-700 13 Heraklion, GreeceInstitute of Molecular Biology & Biotechnology, Foundation for Research & Technology Hellas, 100 N. Plastira Street, GR-700 13 Heraklion, GreeceInstitute of Molecular Biology & Biotechnology, Foundation for Research & Technology Hellas, 100 N. Plastira Street, GR-700 13 Heraklion, GreeceOverexpression of the cytochrome P450 monooxygenase CYP392A16 has been previously associated with abamectin resistance using transcriptional analysis in the two-spotted spider mite <i>Tetranychus urticae</i>, an important pest species worldwide; however, this association has not been functionally validated in vivo despite the demonstrated ability of CYP392A16 to metabolize abamectin in vitro. We expressed CYP392A16 in vivo via a Gal4 transcription activator protein/Upstream Activating Sequence (GAL4/UAS) system in <i>Drosophila melanogaster</i> flies, driving expression with detoxification tissue-specific drivers. We demonstrated that CYP392A16 expression confers statistically significant abamectin resistance in toxicity bioassays in <i>Drosophila</i> only when its homologous redox partner, cytochrome P450 reductase (TuCPR), is co-expressed in transgenic flies. Our study shows that the <i>Drosophila</i> model can be further improved, to facilitate the functional analysis of insecticide resistance mechanisms acting alone or in combination.https://www.mdpi.com/2075-4450/11/12/829detoxificationcytochrome P450cytochrome P450 reductaseabamectin<i>Tetranychus</i>transgenic <i>Drosophila</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria Riga Aris Ilias John Vontas Vassilis Douris |
spellingShingle |
Maria Riga Aris Ilias John Vontas Vassilis Douris Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the <i>Tetranychus urticae</i> CYP392A16-Based Abamectin Resistance in <i>Drosophila</i> Insects detoxification cytochrome P450 cytochrome P450 reductase abamectin <i>Tetranychus</i> transgenic <i>Drosophila</i> |
author_facet |
Maria Riga Aris Ilias John Vontas Vassilis Douris |
author_sort |
Maria Riga |
title |
Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the <i>Tetranychus urticae</i> CYP392A16-Based Abamectin Resistance in <i>Drosophila</i> |
title_short |
Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the <i>Tetranychus urticae</i> CYP392A16-Based Abamectin Resistance in <i>Drosophila</i> |
title_full |
Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the <i>Tetranychus urticae</i> CYP392A16-Based Abamectin Resistance in <i>Drosophila</i> |
title_fullStr |
Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the <i>Tetranychus urticae</i> CYP392A16-Based Abamectin Resistance in <i>Drosophila</i> |
title_full_unstemmed |
Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the <i>Tetranychus urticae</i> CYP392A16-Based Abamectin Resistance in <i>Drosophila</i> |
title_sort |
co-expression of a homologous cytochrome p450 reductase is required for in vivo validation of the <i>tetranychus urticae</i> cyp392a16-based abamectin resistance in <i>drosophila</i> |
publisher |
MDPI AG |
series |
Insects |
issn |
2075-4450 |
publishDate |
2020-11-01 |
description |
Overexpression of the cytochrome P450 monooxygenase CYP392A16 has been previously associated with abamectin resistance using transcriptional analysis in the two-spotted spider mite <i>Tetranychus urticae</i>, an important pest species worldwide; however, this association has not been functionally validated in vivo despite the demonstrated ability of CYP392A16 to metabolize abamectin in vitro. We expressed CYP392A16 in vivo via a Gal4 transcription activator protein/Upstream Activating Sequence (GAL4/UAS) system in <i>Drosophila melanogaster</i> flies, driving expression with detoxification tissue-specific drivers. We demonstrated that CYP392A16 expression confers statistically significant abamectin resistance in toxicity bioassays in <i>Drosophila</i> only when its homologous redox partner, cytochrome P450 reductase (TuCPR), is co-expressed in transgenic flies. Our study shows that the <i>Drosophila</i> model can be further improved, to facilitate the functional analysis of insecticide resistance mechanisms acting alone or in combination. |
topic |
detoxification cytochrome P450 cytochrome P450 reductase abamectin <i>Tetranychus</i> transgenic <i>Drosophila</i> |
url |
https://www.mdpi.com/2075-4450/11/12/829 |
work_keys_str_mv |
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