Quantification of Melittin in Iranian Honey Bee (Apis mellifera meda) Venom by Liquid Chromatography-electrospray Ionization-ion Trap Tandem Mass Spectrometry (LC-ESI-IT-MS/MS)
The current research aimed to quantify melittin (MEL) in Iranian honey bee (<em>Apis mellifera meda</em>) venom. To this end, a liquid chromatography-electrospray ionization<strong>-</strong>ion trap tandem mass spectrometry (LC-ESI-IT-MS/MS) approach was employed. Melittin i...
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doaj-e50efeadd75b4739a1ad1c3ac8f2ba762020-11-25T02:10:16Zeng Razi Vaccine and Serum Research InstituteArchives of Razi Institute 0365-34392008-98722019-12-0174443543910.22092/ari.2018.122150.1219120201Quantification of Melittin in Iranian Honey Bee (Apis mellifera meda) Venom by Liquid Chromatography-electrospray Ionization-ion Trap Tandem Mass Spectrometry (LC-ESI-IT-MS/MS)M. Hematyar0A. Es-haghi1M. Soleimani2Department of Chemistry, Imam Khomeini International University (IKIU), Qazvin, IranDepartment of Physico Chemistry, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranDepartment of Chemistry, Imam Khomeini International University (IKIU), Qazvin, IranThe current research aimed to quantify melittin (MEL) in Iranian honey bee (<em>Apis mellifera meda</em>) venom. To this end, a liquid chromatography-electrospray ionization<strong>-</strong>ion trap tandem mass spectrometry (LC-ESI-IT-MS/MS) approach was employed. Melittin is the main toxic peptide of honey bee venom with various biological and pharmacological activities. It was extracted with pure water from the bee venom samples. The analyses were performed on XBridge BEH300 C4 column using a gradient method with the mobile phase consisting of ultrapure water and acetonitrile (containing 0.1% formic acid). Signals of the melittin were recorded with the selected reaction monitoring (SRM) mode, which is a quantitative approach capable of quantifying analyte peptides with high sensitivity and. The mass spectrum of MEL was obtained in the positive ion mode and the quantification analysis was performed using precursor to product ion transition of m/z 570.2/669.9. This method demonstrated good linearity (R<sup>2</sup>˃0.997) in the range of 1-100 µg mL<sup>-1</sup>, with a limit of quantification (LOQ) of 1.0 µg mL<sup>-1</sup>. The content of MEL in Iranian honey bee venom accounts for 43–55% of total dry weight. This method can be used to evaluate the quality and authenticity of bee venom samples for different therapeutic applications of MEL.https://archrazi.areeo.ac.ir/article_120201_dbe360a7b75247a80ffcb1158d8126a1.pdfapis mellifera medabee venommelittinpeptidelc-esi-it-ms/ms |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M. Hematyar A. Es-haghi M. Soleimani |
spellingShingle |
M. Hematyar A. Es-haghi M. Soleimani Quantification of Melittin in Iranian Honey Bee (Apis mellifera meda) Venom by Liquid Chromatography-electrospray Ionization-ion Trap Tandem Mass Spectrometry (LC-ESI-IT-MS/MS) Archives of Razi Institute apis mellifera meda bee venom melittin peptide lc-esi-it-ms/ms |
author_facet |
M. Hematyar A. Es-haghi M. Soleimani |
author_sort |
M. Hematyar |
title |
Quantification of Melittin in Iranian Honey Bee (Apis mellifera meda) Venom by Liquid Chromatography-electrospray Ionization-ion Trap Tandem Mass Spectrometry (LC-ESI-IT-MS/MS) |
title_short |
Quantification of Melittin in Iranian Honey Bee (Apis mellifera meda) Venom by Liquid Chromatography-electrospray Ionization-ion Trap Tandem Mass Spectrometry (LC-ESI-IT-MS/MS) |
title_full |
Quantification of Melittin in Iranian Honey Bee (Apis mellifera meda) Venom by Liquid Chromatography-electrospray Ionization-ion Trap Tandem Mass Spectrometry (LC-ESI-IT-MS/MS) |
title_fullStr |
Quantification of Melittin in Iranian Honey Bee (Apis mellifera meda) Venom by Liquid Chromatography-electrospray Ionization-ion Trap Tandem Mass Spectrometry (LC-ESI-IT-MS/MS) |
title_full_unstemmed |
Quantification of Melittin in Iranian Honey Bee (Apis mellifera meda) Venom by Liquid Chromatography-electrospray Ionization-ion Trap Tandem Mass Spectrometry (LC-ESI-IT-MS/MS) |
title_sort |
quantification of melittin in iranian honey bee (apis mellifera meda) venom by liquid chromatography-electrospray ionization-ion trap tandem mass spectrometry (lc-esi-it-ms/ms) |
publisher |
Razi Vaccine and Serum Research Institute |
series |
Archives of Razi Institute |
issn |
0365-3439 2008-9872 |
publishDate |
2019-12-01 |
description |
The current research aimed to quantify melittin (MEL) in Iranian honey bee (<em>Apis mellifera meda</em>) venom. To this end, a liquid chromatography-electrospray ionization<strong>-</strong>ion trap tandem mass spectrometry (LC-ESI-IT-MS/MS) approach was employed. Melittin is the main toxic peptide of honey bee venom with various biological and pharmacological activities. It was extracted with pure water from the bee venom samples. The analyses were performed on XBridge BEH300 C4 column using a gradient method with the mobile phase consisting of ultrapure water and acetonitrile (containing 0.1% formic acid). Signals of the melittin were recorded with the selected reaction monitoring (SRM) mode, which is a quantitative approach capable of quantifying analyte peptides with high sensitivity and. The mass spectrum of MEL was obtained in the positive ion mode and the quantification analysis was performed using precursor to product ion transition of m/z 570.2/669.9. This method demonstrated good linearity (R<sup>2</sup>˃0.997) in the range of 1-100 µg mL<sup>-1</sup>, with a limit of quantification (LOQ) of 1.0 µg mL<sup>-1</sup>. The content of MEL in Iranian honey bee venom accounts for 43–55% of total dry weight. This method can be used to evaluate the quality and authenticity of bee venom samples for different therapeutic applications of MEL. |
topic |
apis mellifera meda bee venom melittin peptide lc-esi-it-ms/ms |
url |
https://archrazi.areeo.ac.ir/article_120201_dbe360a7b75247a80ffcb1158d8126a1.pdf |
work_keys_str_mv |
AT mhematyar quantificationofmelittininiranianhoneybeeapismelliferamedavenombyliquidchromatographyelectrosprayionizationiontraptandemmassspectrometrylcesiitmsms AT aeshaghi quantificationofmelittininiranianhoneybeeapismelliferamedavenombyliquidchromatographyelectrosprayionizationiontraptandemmassspectrometrylcesiitmsms AT msoleimani quantificationofmelittininiranianhoneybeeapismelliferamedavenombyliquidchromatographyelectrosprayionizationiontraptandemmassspectrometrylcesiitmsms |
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