Circulating Tumor-Cell-Associated White Blood Cell Clusters in Peripheral Blood Indicate Poor Prognosis in Patients With Hepatocellular Carcinoma
Aim: Circulating tumor cells (CTC) are a precursor to metastasis in several types of cancer and are occasionally found in the bloodstream in association with immune cells, such as white blood cells (WBCs). CTC-associated WBC (CTC-WBC) clusters can promote CTC appreciation and metastasis, suggesting...
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Frontiers Media S.A.
2020-11-01
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doaj-e519d8730648452bbeb4a7281d7f2be52020-11-25T04:02:37ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-11-011010.3389/fonc.2020.01758573428Circulating Tumor-Cell-Associated White Blood Cell Clusters in Peripheral Blood Indicate Poor Prognosis in Patients With Hepatocellular CarcinomaQiong Luo0Qiong Luo1Chunming Wang2Bangjian Peng3Xiaoyu Pu4Lei Cai5Hangyu Liao6Kunling Chen7Cheng Zhang8Yuan Cheng9Mingxin Pan10Second Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of General Surgery, Affiliated Hengyang Hospital, Southern Medical University (Hengyang Central Hospital), Hengyang, ChinaSecond Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Hepatobiliary Surgery, The Fifth Affiliated Hospital of Southern Medical University, Guangzhou, ChinaSurExam Bio-Tech, Guangzhou Technology Innovation Base, Guangzhou, ChinaSecond Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaSecond Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaSecond Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaSecond Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaSecond Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaSecond Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaAim: Circulating tumor cells (CTC) are a precursor to metastasis in several types of cancer and are occasionally found in the bloodstream in association with immune cells, such as white blood cells (WBCs). CTC-associated WBC (CTC-WBC) clusters can promote CTC appreciation and metastasis, suggesting that patients with CTC-WBC clusters found in the peripheral blood may have a worse prognosis. However, it is unclear whether CTC-WBC clusters are present in the peripheral blood of patients with hepatocellular carcinoma (HCC) and suggest a poor prognosis for HCC.Methods: We collected peripheral blood from 214 patients with HCC from January 2014 to December 2016. CanPatrol™ CTC analysis technology was used to isolate and count CTCs and CTC-WBC clusters in the patients' peripheral blood. Chi-squared analysis was used to calculate the correlation between the CTC-WBC clusters and clinicopathological characteristics. Kaplan–Meier survival analysis and Cox regression analysis were used to assess patient prognosis.Results: We used CanPatrol™ CTC analysis technology to count different types of CTCs and CTC-WBC clusters. The results showed that CTC-WBC clusters and tumor size (P = 0.001), tumor number (P = 0.005), portal vein tumor thrombus (P = 0.026), BCLC stage (P < 0.001), AFP level (P = 0.002), and total number of CTCs (P < 0.001) were statistically related. Cox regression analysis revealed that CTC-WBC clusters are an independent prognostic indicator of DFS (HR = 1.951, 95%CI:1.348–2.824, P < 0.001) and OS (HR = 3.026, 95%CI:1.906–4.802, P < 0.001) in HCC patients. Using Kaplan–Meier analysis, we found that positive CTC-WBC cluster patients had significantly shorter DFS and OS than patients with negative CTC-WBC (P < 0.001 and P < 0.001, respectively).Conclusions: CTC-WBC clusters in the peripheral blood are an independent predictor of DFS and OS, and their presence indicates poor prognosis in patients with HCC.https://www.frontiersin.org/articles/10.3389/fonc.2020.01758/fullCTC-WBC clusterhepatocellular carcinomaprognosisKaplan-Meier plotcirculating tumor cells |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qiong Luo Qiong Luo Chunming Wang Bangjian Peng Xiaoyu Pu Lei Cai Hangyu Liao Kunling Chen Cheng Zhang Yuan Cheng Mingxin Pan |
spellingShingle |
Qiong Luo Qiong Luo Chunming Wang Bangjian Peng Xiaoyu Pu Lei Cai Hangyu Liao Kunling Chen Cheng Zhang Yuan Cheng Mingxin Pan Circulating Tumor-Cell-Associated White Blood Cell Clusters in Peripheral Blood Indicate Poor Prognosis in Patients With Hepatocellular Carcinoma Frontiers in Oncology CTC-WBC cluster hepatocellular carcinoma prognosis Kaplan-Meier plot circulating tumor cells |
author_facet |
Qiong Luo Qiong Luo Chunming Wang Bangjian Peng Xiaoyu Pu Lei Cai Hangyu Liao Kunling Chen Cheng Zhang Yuan Cheng Mingxin Pan |
author_sort |
Qiong Luo |
title |
Circulating Tumor-Cell-Associated White Blood Cell Clusters in Peripheral Blood Indicate Poor Prognosis in Patients With Hepatocellular Carcinoma |
title_short |
Circulating Tumor-Cell-Associated White Blood Cell Clusters in Peripheral Blood Indicate Poor Prognosis in Patients With Hepatocellular Carcinoma |
title_full |
Circulating Tumor-Cell-Associated White Blood Cell Clusters in Peripheral Blood Indicate Poor Prognosis in Patients With Hepatocellular Carcinoma |
title_fullStr |
Circulating Tumor-Cell-Associated White Blood Cell Clusters in Peripheral Blood Indicate Poor Prognosis in Patients With Hepatocellular Carcinoma |
title_full_unstemmed |
Circulating Tumor-Cell-Associated White Blood Cell Clusters in Peripheral Blood Indicate Poor Prognosis in Patients With Hepatocellular Carcinoma |
title_sort |
circulating tumor-cell-associated white blood cell clusters in peripheral blood indicate poor prognosis in patients with hepatocellular carcinoma |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Oncology |
issn |
2234-943X |
publishDate |
2020-11-01 |
description |
Aim: Circulating tumor cells (CTC) are a precursor to metastasis in several types of cancer and are occasionally found in the bloodstream in association with immune cells, such as white blood cells (WBCs). CTC-associated WBC (CTC-WBC) clusters can promote CTC appreciation and metastasis, suggesting that patients with CTC-WBC clusters found in the peripheral blood may have a worse prognosis. However, it is unclear whether CTC-WBC clusters are present in the peripheral blood of patients with hepatocellular carcinoma (HCC) and suggest a poor prognosis for HCC.Methods: We collected peripheral blood from 214 patients with HCC from January 2014 to December 2016. CanPatrol™ CTC analysis technology was used to isolate and count CTCs and CTC-WBC clusters in the patients' peripheral blood. Chi-squared analysis was used to calculate the correlation between the CTC-WBC clusters and clinicopathological characteristics. Kaplan–Meier survival analysis and Cox regression analysis were used to assess patient prognosis.Results: We used CanPatrol™ CTC analysis technology to count different types of CTCs and CTC-WBC clusters. The results showed that CTC-WBC clusters and tumor size (P = 0.001), tumor number (P = 0.005), portal vein tumor thrombus (P = 0.026), BCLC stage (P < 0.001), AFP level (P = 0.002), and total number of CTCs (P < 0.001) were statistically related. Cox regression analysis revealed that CTC-WBC clusters are an independent prognostic indicator of DFS (HR = 1.951, 95%CI:1.348–2.824, P < 0.001) and OS (HR = 3.026, 95%CI:1.906–4.802, P < 0.001) in HCC patients. Using Kaplan–Meier analysis, we found that positive CTC-WBC cluster patients had significantly shorter DFS and OS than patients with negative CTC-WBC (P < 0.001 and P < 0.001, respectively).Conclusions: CTC-WBC clusters in the peripheral blood are an independent predictor of DFS and OS, and their presence indicates poor prognosis in patients with HCC. |
topic |
CTC-WBC cluster hepatocellular carcinoma prognosis Kaplan-Meier plot circulating tumor cells |
url |
https://www.frontiersin.org/articles/10.3389/fonc.2020.01758/full |
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