Predictive Criteria to Study the Pathogenesis of Malaria-Associated ALI/ARDS in Mice
Malaria-associated acute lung injury/acute respiratory distress syndrome (ALI/ARDS) often results in morbidity and mortality. Murine models to study malaria-associated ALI/ARDS have been described; we still lack a method of distinguishing which mice will develop ALI/ARDS before death. This work aime...
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Format: | Article |
Language: | English |
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Hindawi Limited
2014-01-01
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Series: | Mediators of Inflammation |
Online Access: | http://dx.doi.org/10.1155/2014/872464 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Luana S. Ortolan Michelle K. Sercundes Renato Barboza Daniela Debone Oscar Murillo Stefano C. F. Hagen Momtchilo Russo Maria Regina D’ Império Lima José M. Alvarez Marcos Amaku Claudio R. F. Marinho Sabrina Epiphanio |
spellingShingle |
Luana S. Ortolan Michelle K. Sercundes Renato Barboza Daniela Debone Oscar Murillo Stefano C. F. Hagen Momtchilo Russo Maria Regina D’ Império Lima José M. Alvarez Marcos Amaku Claudio R. F. Marinho Sabrina Epiphanio Predictive Criteria to Study the Pathogenesis of Malaria-Associated ALI/ARDS in Mice Mediators of Inflammation |
author_facet |
Luana S. Ortolan Michelle K. Sercundes Renato Barboza Daniela Debone Oscar Murillo Stefano C. F. Hagen Momtchilo Russo Maria Regina D’ Império Lima José M. Alvarez Marcos Amaku Claudio R. F. Marinho Sabrina Epiphanio |
author_sort |
Luana S. Ortolan |
title |
Predictive Criteria to Study the Pathogenesis of Malaria-Associated ALI/ARDS in Mice |
title_short |
Predictive Criteria to Study the Pathogenesis of Malaria-Associated ALI/ARDS in Mice |
title_full |
Predictive Criteria to Study the Pathogenesis of Malaria-Associated ALI/ARDS in Mice |
title_fullStr |
Predictive Criteria to Study the Pathogenesis of Malaria-Associated ALI/ARDS in Mice |
title_full_unstemmed |
Predictive Criteria to Study the Pathogenesis of Malaria-Associated ALI/ARDS in Mice |
title_sort |
predictive criteria to study the pathogenesis of malaria-associated ali/ards in mice |
publisher |
Hindawi Limited |
series |
Mediators of Inflammation |
issn |
0962-9351 1466-1861 |
publishDate |
2014-01-01 |
description |
Malaria-associated acute lung injury/acute respiratory distress syndrome (ALI/ARDS) often results in morbidity and mortality. Murine models to study malaria-associated ALI/ARDS have been described; we still lack a method of distinguishing which mice will develop ALI/ARDS before death. This work aimed to characterize malaria-associated ALI/ARDS in a murine model and to demonstrate the first method to predict whether mice are suffering from ALI/ARDS before death. DBA/2 mice infected with Plasmodium berghei ANKA developing ALI/ARDS or hyperparasitemia (HP) were compared using histopathology, PaO2 measurement, pulmonary X-ray, breathing capacity, lung permeability, and serum vascular endothelial growth factor (VEGF) levels according to either the day of death or the suggested predictive criteria. We proposed a model to predict malaria-associated ALI/ARDS using breathing patterns (enhanced pause and frequency respiration) and parasitemia as predictive criteria from mice whose cause of death was known to retrospectively diagnose the sacrificed mice as likely to die of ALI/ARDS as early as 7 days after infection. Using this method, we showed increased VEGF levels and increased lung permeability in mice predicted to die of ALI/ARDS. This proposed method for accurately identifying mice suffering from ALI/ARDS before death will enable the use of this model to study the pathogenesis of this disease. |
url |
http://dx.doi.org/10.1155/2014/872464 |
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doaj-e51fdacc3d004f53b7cfa22ec8b6fe152020-11-24T21:33:10ZengHindawi LimitedMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/872464872464Predictive Criteria to Study the Pathogenesis of Malaria-Associated ALI/ARDS in MiceLuana S. Ortolan0Michelle K. Sercundes1Renato Barboza2Daniela Debone3Oscar Murillo4Stefano C. F. Hagen5Momtchilo Russo6Maria Regina D’ Império Lima7José M. Alvarez8Marcos Amaku9Claudio R. F. Marinho10Sabrina Epiphanio11Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Edifício Biomédicas IV, Avenida Professor Lineu Prestes, No. 1730, 05508-900 São Paulo, SP, BrazilInstituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Avenida Dr. Enéas Carvalho de Aguiar, No. 470, 05403-000 São Paulo, SP, BrazilDepartamento de Ciências Exatas e da Terra, Universidade Federal de São Paulo, Rua Professor Artur Riedel, No. 275, Jardim Eldorado, 09972-270 Diadema, SP, BrazilInstituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Avenida Dr. Enéas Carvalho de Aguiar, No. 470, 05403-000 São Paulo, SP, BrazilDepartamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Avenida Professor Lineu Prestes, No. 1374, Edifício Biomédicas II Cidade Universitária “Armando Salles Oliveira”, 05508-000 São Paulo, SP, BrazilDepartamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Avenida Professor Dr. Orlando Marques de Paiva, No. 87, Cidade Universitária, 05508 270 São Paulo, SP, BrazilDepartamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Edifício Biomédicas IV, Avenida Professor Lineu Prestes, No. 1730, 05508-900 São Paulo, SP, BrazilDepartamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Edifício Biomédicas IV, Avenida Professor Lineu Prestes, No. 1730, 05508-900 São Paulo, SP, BrazilDepartamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Edifício Biomédicas IV, Avenida Professor Lineu Prestes, No. 1730, 05508-900 São Paulo, SP, BrazilDepartamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Avenida Professor Dr. Orlando Marques de Paiva, No. 87, Cidade Universitária, 05508 270 São Paulo, SP, BrazilDepartamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Avenida Professor Lineu Prestes, No. 1374, Edifício Biomédicas II Cidade Universitária “Armando Salles Oliveira”, 05508-000 São Paulo, SP, BrazilDepartamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Edifício Biomédicas IV, Avenida Professor Lineu Prestes, No. 1730, 05508-900 São Paulo, SP, BrazilMalaria-associated acute lung injury/acute respiratory distress syndrome (ALI/ARDS) often results in morbidity and mortality. Murine models to study malaria-associated ALI/ARDS have been described; we still lack a method of distinguishing which mice will develop ALI/ARDS before death. This work aimed to characterize malaria-associated ALI/ARDS in a murine model and to demonstrate the first method to predict whether mice are suffering from ALI/ARDS before death. DBA/2 mice infected with Plasmodium berghei ANKA developing ALI/ARDS or hyperparasitemia (HP) were compared using histopathology, PaO2 measurement, pulmonary X-ray, breathing capacity, lung permeability, and serum vascular endothelial growth factor (VEGF) levels according to either the day of death or the suggested predictive criteria. We proposed a model to predict malaria-associated ALI/ARDS using breathing patterns (enhanced pause and frequency respiration) and parasitemia as predictive criteria from mice whose cause of death was known to retrospectively diagnose the sacrificed mice as likely to die of ALI/ARDS as early as 7 days after infection. Using this method, we showed increased VEGF levels and increased lung permeability in mice predicted to die of ALI/ARDS. This proposed method for accurately identifying mice suffering from ALI/ARDS before death will enable the use of this model to study the pathogenesis of this disease.http://dx.doi.org/10.1155/2014/872464 |