Unraveling Binding Mechanism of Alzheimer’s Drug Rivastigmine Tartrate with Human Transferrin: Molecular Docking and Multi-Spectroscopic Approach towards Neurodegenerative Diseases

• Main Authors: Anas Shamsi, Taj Mohammad, Mohd Shahnawaz Khan, Moyad Shahwan, Fohad Mabood Husain, Md. Tabish Rehman, Md. Imtaiyaz Hassan, Faizan Ahmad, Asimul Islam
• Format: Article
• Language: English
• Published: MDPI AG 2019-09-01
• Series: Biomolecules
• Subjects: human transferrin; rivastigmine tartrate; spectroscopy; molecular docking; isotheral titration calorimetry; Alzheimer’s disease; neurodegenerative disorders
• Online Access: https://www.mdpi.com/2218-273X/9/9/495

Studying drug−protein interactions has gained significant attention lately, and this is because the majority of drugs interact with proteins, thereby altering their structure and, moreover, their functionality. Rivastigmine tartrate (RT) is a drug that is in use for mild to moderate Alzheimer therapy. This study was targeted to characterize the interaction between human transferrin (hTf) and RT by employing spectroscopy, isothermal titration calorimetry (ITC), and molecular docking studies. Experimental results of fluorescence quenching of hTf induced by RT implied the formation of a static complex between hTf and RT. Further elucidation of the observed fluorescence data retorting Stern−Volmer and modified Stern−Volmer resulted in binding constants for hTf−RT complex of the order 10⁴ M^−1 over the studied temperatures. Thermodynamic parameters of hTf−RT interaction were elucidated further by employing these obtained binding constant values. It was quite evident from obtained thermodynamic attributes that RT spontaneously binds to hTf with a postulated existence of hydrogen bonding or Van der Waals forces. Further, Circular dichroism spectroscopy (CD) also confirmed RT−hTf complex formation owing to upward movement of CD spectra in the presence of RT. ITC profiles advocated the existence of reaction to be spontaneous. Moreover, molecular docking further revealed that the important residues play a pivotal role in RT−hTf interaction. The findings of this study can be of a significant benefit to the drug-designing industry in this disease-prone era.