Semen Modulates Inflammation and Angiogenesis in the Reproductive Tract of Female Rabbits
The maternal environment modulates immune responses to facilitate embryo development and ensure pregnancy. Unraveling this modulation could improve the livestock breeding systems. Here it is hypothesized that the exposure of the female rabbit reproductive tract to semen, as well as to early embryos,...
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doaj-e546850a5e38463f9332fa3ed3ae59512020-11-27T08:02:58ZengMDPI AGAnimals2076-26152020-11-01102207220710.3390/ani10122207Semen Modulates Inflammation and Angiogenesis in the Reproductive Tract of Female RabbitsJaume Gardela0Amaia Jauregi-Miguel1Cristina A. Martinez2Heriberto Rodríguez-Martinez3Manel López-Béjar4Manuel Álvarez-Rodríguez5Department of Biomedical and Clinical Sciences (BKV), Division of Children’s and Women Health (BKH), Obstetrics and Gynecology, Linköping University, 58185 Linköping, SwedenDivision of Molecular Medicine and Virology (MMV), Linköping University, 58185 Linköping, SwedenDepartment of Biomedical and Clinical Sciences (BKV), Division of Children’s and Women Health (BKH), Obstetrics and Gynecology, Linköping University, 58185 Linköping, SwedenDepartment of Biomedical and Clinical Sciences (BKV), Division of Children’s and Women Health (BKH), Obstetrics and Gynecology, Linköping University, 58185 Linköping, SwedenDepartment of Animal Health and Anatomy, Veterinary Faculty, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDepartment of Biomedical and Clinical Sciences (BKV), Division of Children’s and Women Health (BKH), Obstetrics and Gynecology, Linköping University, 58185 Linköping, SwedenThe maternal environment modulates immune responses to facilitate embryo development and ensure pregnancy. Unraveling this modulation could improve the livestock breeding systems. Here it is hypothesized that the exposure of the female rabbit reproductive tract to semen, as well as to early embryos, modulates inflammation and angiogenesis among different tissue segments. qPCR analysis of the gene expression changes of the anti-inflammatory interleukin-10 (IL10) and transforming growth factor beta family (TGFβ1–3) and the angiogenesis mediator vascular endothelial growth factor (VEGF-A) were examined in response to mating or insemination with sperm-free seminal plasma (SP). Reproductive tract segment (cervix to infundibulum) samples were obtained in Experiment 1, 20 h after gonadotropin-releasing hormone (GnRH) stimulation (control), natural mating (NM) or vaginal infusion with sperm-free SP (SP-AI). Additionally, segmented samples were also obtained at 10, 24, 36, 68 or 72 h after GnRH-stimulation and natural mating (Experiment 2). The results of gene expression, analyzed by quantitative PCR, showed that NM effects were mainly localized in the uterine tissues, depicting clear temporal variation, while SP-AI effects were restricted to the oviduct. Changes in anti-inflammatory and angiogenesis mediators indicate an early response in the uterus and a late modulation in the oviduct either induced by semen or preimplantation embryos. This knowledge could be used in the implementation of physiological strategies in breeding systems to face the new challenges on rabbit productivity and sustainability.https://www.mdpi.com/2076-2615/10/12/2207gene expressionendometriumoviductspermatozoaseminal plasmainflammation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jaume Gardela Amaia Jauregi-Miguel Cristina A. Martinez Heriberto Rodríguez-Martinez Manel López-Béjar Manuel Álvarez-Rodríguez |
spellingShingle |
Jaume Gardela Amaia Jauregi-Miguel Cristina A. Martinez Heriberto Rodríguez-Martinez Manel López-Béjar Manuel Álvarez-Rodríguez Semen Modulates Inflammation and Angiogenesis in the Reproductive Tract of Female Rabbits Animals gene expression endometrium oviduct spermatozoa seminal plasma inflammation |
author_facet |
Jaume Gardela Amaia Jauregi-Miguel Cristina A. Martinez Heriberto Rodríguez-Martinez Manel López-Béjar Manuel Álvarez-Rodríguez |
author_sort |
Jaume Gardela |
title |
Semen Modulates Inflammation and Angiogenesis in the Reproductive Tract of Female Rabbits |
title_short |
Semen Modulates Inflammation and Angiogenesis in the Reproductive Tract of Female Rabbits |
title_full |
Semen Modulates Inflammation and Angiogenesis in the Reproductive Tract of Female Rabbits |
title_fullStr |
Semen Modulates Inflammation and Angiogenesis in the Reproductive Tract of Female Rabbits |
title_full_unstemmed |
Semen Modulates Inflammation and Angiogenesis in the Reproductive Tract of Female Rabbits |
title_sort |
semen modulates inflammation and angiogenesis in the reproductive tract of female rabbits |
publisher |
MDPI AG |
series |
Animals |
issn |
2076-2615 |
publishDate |
2020-11-01 |
description |
The maternal environment modulates immune responses to facilitate embryo development and ensure pregnancy. Unraveling this modulation could improve the livestock breeding systems. Here it is hypothesized that the exposure of the female rabbit reproductive tract to semen, as well as to early embryos, modulates inflammation and angiogenesis among different tissue segments. qPCR analysis of the gene expression changes of the anti-inflammatory interleukin-10 (IL10) and transforming growth factor beta family (TGFβ1–3) and the angiogenesis mediator vascular endothelial growth factor (VEGF-A) were examined in response to mating or insemination with sperm-free seminal plasma (SP). Reproductive tract segment (cervix to infundibulum) samples were obtained in Experiment 1, 20 h after gonadotropin-releasing hormone (GnRH) stimulation (control), natural mating (NM) or vaginal infusion with sperm-free SP (SP-AI). Additionally, segmented samples were also obtained at 10, 24, 36, 68 or 72 h after GnRH-stimulation and natural mating (Experiment 2). The results of gene expression, analyzed by quantitative PCR, showed that NM effects were mainly localized in the uterine tissues, depicting clear temporal variation, while SP-AI effects were restricted to the oviduct. Changes in anti-inflammatory and angiogenesis mediators indicate an early response in the uterus and a late modulation in the oviduct either induced by semen or preimplantation embryos. This knowledge could be used in the implementation of physiological strategies in breeding systems to face the new challenges on rabbit productivity and sustainability. |
topic |
gene expression endometrium oviduct spermatozoa seminal plasma inflammation |
url |
https://www.mdpi.com/2076-2615/10/12/2207 |
work_keys_str_mv |
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