Fabrication of Gentamicin-Loaded Hydroxyapatite/Collagen Bone-Like Nanocomposite for Anti-Infection Bone Void Fillers
A gentamicin-loaded hydroxyapatite/collagen bone-like nanocomposite (GNT-HAp/Col) was fabricated and evaluated for its absorption−desorption properties, antibacterial efficacy, and cytotoxicity. The hydroxyapatite/collagen bone-like nanocomposite (HAp/Col) powder was mixed with gentamicin...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-01-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/21/2/551 |
id |
doaj-e56117f38c0d4ed29cb65313f6811274 |
---|---|
record_format |
Article |
spelling |
doaj-e56117f38c0d4ed29cb65313f68112742020-11-25T01:38:06ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-01-0121255110.3390/ijms21020551ijms21020551Fabrication of Gentamicin-Loaded Hydroxyapatite/Collagen Bone-Like Nanocomposite for Anti-Infection Bone Void FillersSho Oshima0Taira Sato1Michiyo Honda2Yasushi Suetsugu3Kazuhide Ozeki4Masanori Kikuchi5Major in Industrial Science, Graduate School of Science and Engineering, Ibaraki University, 4-12-1 Nakanarusawa, Hitachi, Ibaraki 316-8511, JapanBioceramics Group, Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, JapanDepartment of Applied Chemistry, School of Science and Technology, Meiji University, 1-1-1 Higashimita, Tama, Kawasaki, Kanagawa 214-8571, JapanBioceramics Group, Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, JapanMajor in Mechanical Systems Engineering, Graduate School of Science and Engineering, Ibaraki University, 4-12-1 Nakanarusawa, Hitachi, Ibaraki 316-8511, JapanBioceramics Group, Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, JapanA gentamicin-loaded hydroxyapatite/collagen bone-like nanocomposite (GNT-HAp/Col) was fabricated and evaluated for its absorption−desorption properties, antibacterial efficacy, and cytotoxicity. The hydroxyapatite/collagen bone-like nanocomposite (HAp/Col) powder was mixed with gentamicin sulfate (GNT) in phosphate-buffered saline (PBS) at room temperature. After 6 h mixing, the GNT adsorption in all conditions reached plateau by Langmuir’s isotherm, and maximum GNT adsorption amount was 34 ± 7 μg in 250 μg/mL GNT solution. Saturated GNT-loaded HAp/Col powder of 100 mg was soaked in 10 mL of PBS at 37 °C and released all GNT in 3 days. A shaking culture method for a GNT extraction from the GNT-HAp/Col and an inhibition zone assay for the GNT-HAp/Col compact showed antibacterial efficacy to <i>Escherichia coli</i> (<i>E. coli</i>) at least for 2 days. From the release profile of the GNT from the GNT-HAp/Col powder, antibacterial efficacy would affect <i>E. coli</i> at least for 3 days. Further, no cytotoxicities were observed on MG-63 cells. Thus, the GNT-HAp/Col is a good candidate of bioresorbable anti-infection bone void fillers by prevention initial infections, which is the primary cause of implant-associated infection even for rapid bioresorbable materials.https://www.mdpi.com/1422-0067/21/2/551antibiotic-impregnated biomaterialhydroxyapatite/collagen bone-like nanocompositegentamicin sulfatebone void fillerantibacterial activity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sho Oshima Taira Sato Michiyo Honda Yasushi Suetsugu Kazuhide Ozeki Masanori Kikuchi |
spellingShingle |
Sho Oshima Taira Sato Michiyo Honda Yasushi Suetsugu Kazuhide Ozeki Masanori Kikuchi Fabrication of Gentamicin-Loaded Hydroxyapatite/Collagen Bone-Like Nanocomposite for Anti-Infection Bone Void Fillers International Journal of Molecular Sciences antibiotic-impregnated biomaterial hydroxyapatite/collagen bone-like nanocomposite gentamicin sulfate bone void filler antibacterial activity |
author_facet |
Sho Oshima Taira Sato Michiyo Honda Yasushi Suetsugu Kazuhide Ozeki Masanori Kikuchi |
author_sort |
Sho Oshima |
title |
Fabrication of Gentamicin-Loaded Hydroxyapatite/Collagen Bone-Like Nanocomposite for Anti-Infection Bone Void Fillers |
title_short |
Fabrication of Gentamicin-Loaded Hydroxyapatite/Collagen Bone-Like Nanocomposite for Anti-Infection Bone Void Fillers |
title_full |
Fabrication of Gentamicin-Loaded Hydroxyapatite/Collagen Bone-Like Nanocomposite for Anti-Infection Bone Void Fillers |
title_fullStr |
Fabrication of Gentamicin-Loaded Hydroxyapatite/Collagen Bone-Like Nanocomposite for Anti-Infection Bone Void Fillers |
title_full_unstemmed |
Fabrication of Gentamicin-Loaded Hydroxyapatite/Collagen Bone-Like Nanocomposite for Anti-Infection Bone Void Fillers |
title_sort |
fabrication of gentamicin-loaded hydroxyapatite/collagen bone-like nanocomposite for anti-infection bone void fillers |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-01-01 |
description |
A gentamicin-loaded hydroxyapatite/collagen bone-like nanocomposite (GNT-HAp/Col) was fabricated and evaluated for its absorption−desorption properties, antibacterial efficacy, and cytotoxicity. The hydroxyapatite/collagen bone-like nanocomposite (HAp/Col) powder was mixed with gentamicin sulfate (GNT) in phosphate-buffered saline (PBS) at room temperature. After 6 h mixing, the GNT adsorption in all conditions reached plateau by Langmuir’s isotherm, and maximum GNT adsorption amount was 34 ± 7 μg in 250 μg/mL GNT solution. Saturated GNT-loaded HAp/Col powder of 100 mg was soaked in 10 mL of PBS at 37 °C and released all GNT in 3 days. A shaking culture method for a GNT extraction from the GNT-HAp/Col and an inhibition zone assay for the GNT-HAp/Col compact showed antibacterial efficacy to <i>Escherichia coli</i> (<i>E. coli</i>) at least for 2 days. From the release profile of the GNT from the GNT-HAp/Col powder, antibacterial efficacy would affect <i>E. coli</i> at least for 3 days. Further, no cytotoxicities were observed on MG-63 cells. Thus, the GNT-HAp/Col is a good candidate of bioresorbable anti-infection bone void fillers by prevention initial infections, which is the primary cause of implant-associated infection even for rapid bioresorbable materials. |
topic |
antibiotic-impregnated biomaterial hydroxyapatite/collagen bone-like nanocomposite gentamicin sulfate bone void filler antibacterial activity |
url |
https://www.mdpi.com/1422-0067/21/2/551 |
work_keys_str_mv |
AT shooshima fabricationofgentamicinloadedhydroxyapatitecollagenbonelikenanocompositeforantiinfectionbonevoidfillers AT tairasato fabricationofgentamicinloadedhydroxyapatitecollagenbonelikenanocompositeforantiinfectionbonevoidfillers AT michiyohonda fabricationofgentamicinloadedhydroxyapatitecollagenbonelikenanocompositeforantiinfectionbonevoidfillers AT yasushisuetsugu fabricationofgentamicinloadedhydroxyapatitecollagenbonelikenanocompositeforantiinfectionbonevoidfillers AT kazuhideozeki fabricationofgentamicinloadedhydroxyapatitecollagenbonelikenanocompositeforantiinfectionbonevoidfillers AT masanorikikuchi fabricationofgentamicinloadedhydroxyapatitecollagenbonelikenanocompositeforantiinfectionbonevoidfillers |
_version_ |
1725055116399083520 |