Label-Free Direct Detection of miRNAs with Poly-Silicon Nanowire Biosensors.
The diagnostic and prognostic value of microRNAs (miRNAs) in a variety of diseases is promising. The novel silicon nanowire (SiNW) biosensors have advantages in molecular detection because of their high sensitivity and fast response. In this study, poly-crystalline silicon nanowire field-effect tran...
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doaj-e57b590621dd4474ac113081e3aa46ec2020-11-25T01:51:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011012e014516010.1371/journal.pone.0145160Label-Free Direct Detection of miRNAs with Poly-Silicon Nanowire Biosensors.Jing HeJianjun ZhuChangguo GongJiming QiHan XiaoBin JiangYulan ZhaoThe diagnostic and prognostic value of microRNAs (miRNAs) in a variety of diseases is promising. The novel silicon nanowire (SiNW) biosensors have advantages in molecular detection because of their high sensitivity and fast response. In this study, poly-crystalline silicon nanowire field-effect transistor (poly-SiNW FET) device was developed to achieve specific and ultrasensitive detection of miRNAs without labeling and amplification.The poly-SiNW FET was fabricated by a top-down Complementary Metal Oxide Semiconductor (CMOS) wafer fabrication based technique. Single strand DNA (ssDNA) probe was bind to the surface of the poly-SiNW device which was silanated and aldehyde-modified. By comparing the difference of resistance value before and after ssDNA and miRNA hybridization, poly-SiNW device can be used to detect standard and real miRNA samples.Poly-SiNW device with different structures (different line width and different pitch) was applied to detect standard Let-7b sample with a detection limitation of 1 fM. One-base mismatched sequence could be distinguished meanwhile. Furthermore, these poly-SiNW arrays can detect snRNA U6 in total RNA samples extracted from HepG2 cells with a detection limitation of 0.2 μg/mL. In general, structures with pitch showed better results than those without pitch in detection of both Let-7b and snRNA U6. Moreover, structures with smaller pitch showed better detection efficacy.Our findings suggest that poly-SiNW arrays could detect standard and real miRNA sample without labeling or amplification. Poly-SiNW biosensor device is promising for miRNA detection.http://europepmc.org/articles/PMC4692481?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jing He Jianjun Zhu Changguo Gong Jiming Qi Han Xiao Bin Jiang Yulan Zhao |
spellingShingle |
Jing He Jianjun Zhu Changguo Gong Jiming Qi Han Xiao Bin Jiang Yulan Zhao Label-Free Direct Detection of miRNAs with Poly-Silicon Nanowire Biosensors. PLoS ONE |
author_facet |
Jing He Jianjun Zhu Changguo Gong Jiming Qi Han Xiao Bin Jiang Yulan Zhao |
author_sort |
Jing He |
title |
Label-Free Direct Detection of miRNAs with Poly-Silicon Nanowire Biosensors. |
title_short |
Label-Free Direct Detection of miRNAs with Poly-Silicon Nanowire Biosensors. |
title_full |
Label-Free Direct Detection of miRNAs with Poly-Silicon Nanowire Biosensors. |
title_fullStr |
Label-Free Direct Detection of miRNAs with Poly-Silicon Nanowire Biosensors. |
title_full_unstemmed |
Label-Free Direct Detection of miRNAs with Poly-Silicon Nanowire Biosensors. |
title_sort |
label-free direct detection of mirnas with poly-silicon nanowire biosensors. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
The diagnostic and prognostic value of microRNAs (miRNAs) in a variety of diseases is promising. The novel silicon nanowire (SiNW) biosensors have advantages in molecular detection because of their high sensitivity and fast response. In this study, poly-crystalline silicon nanowire field-effect transistor (poly-SiNW FET) device was developed to achieve specific and ultrasensitive detection of miRNAs without labeling and amplification.The poly-SiNW FET was fabricated by a top-down Complementary Metal Oxide Semiconductor (CMOS) wafer fabrication based technique. Single strand DNA (ssDNA) probe was bind to the surface of the poly-SiNW device which was silanated and aldehyde-modified. By comparing the difference of resistance value before and after ssDNA and miRNA hybridization, poly-SiNW device can be used to detect standard and real miRNA samples.Poly-SiNW device with different structures (different line width and different pitch) was applied to detect standard Let-7b sample with a detection limitation of 1 fM. One-base mismatched sequence could be distinguished meanwhile. Furthermore, these poly-SiNW arrays can detect snRNA U6 in total RNA samples extracted from HepG2 cells with a detection limitation of 0.2 μg/mL. In general, structures with pitch showed better results than those without pitch in detection of both Let-7b and snRNA U6. Moreover, structures with smaller pitch showed better detection efficacy.Our findings suggest that poly-SiNW arrays could detect standard and real miRNA sample without labeling or amplification. Poly-SiNW biosensor device is promising for miRNA detection. |
url |
http://europepmc.org/articles/PMC4692481?pdf=render |
work_keys_str_mv |
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