Novel DnaJ Protein Facilitates Thermotolerance of Transgenic Tomatoes
DnaJ proteins, which are molecular chaperones that are widely present in plants, can respond to various environmental stresses. At present, the function of DnaJ proteins was studied in many plant species, but only a few studies were conducted in tomato. Here, we examined the functions of a novel tom...
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doaj-e583dacfb3cf47558fb55ffa54a74adc2020-11-24T21:25:53ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-01-0120236710.3390/ijms20020367ijms20020367Novel DnaJ Protein Facilitates Thermotolerance of Transgenic TomatoesGuodong Wang0Guohua Cai1Na Xu2Litao Zhang3Xiuling Sun4Jing Guan5Qingwei Meng6College of Biological Science, Jining Medical University, Ri’zhao 276800, Shandong, ChinaCollege of Life Science, Nanjing University, Nan’jing 210046, Jiangshu, ChinaCollege of Biological Science, Jining Medical University, Ri’zhao 276800, Shandong, ChinaCollege of Biological Science, Jining Medical University, Ri’zhao 276800, Shandong, ChinaCollege of Biological Science, Jining Medical University, Ri’zhao 276800, Shandong, ChinaCollege of Biological Science, Jining Medical University, Ri’zhao 276800, Shandong, ChinaCollege of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, Shandong, ChinaDnaJ proteins, which are molecular chaperones that are widely present in plants, can respond to various environmental stresses. At present, the function of DnaJ proteins was studied in many plant species, but only a few studies were conducted in tomato. Here, we examined the functions of a novel tomato (Solanum lycopersicum) DnaJ protein (SlDnaJ20) in heat tolerance using sense and antisense transgenic tomatoes. Transient conversion assays of Arabidopsis protoplasts showed that SlDnaJ20 was targeted to chloroplasts. Expression analysis showed that SlDnaJ20 expression was induced by chilling, NaCl, polyethylene glycol, and H2O2, especially via heat stress. Under heat stress, sense plants showed higher fresh weights, chlorophyll content, fluorescence (Fv/Fm), and D1 protein levels, and a lower accumulation of reactive oxygen species (ROS) than antisense plants. These results suggest that SlDnaJ20 overexpression can reduce the photoinhibition of photosystem II (PSII) by relieving ROS accumulation. Moreover, higher expression levels of HsfA1 and HsfB1 were observed under heat stress in sense plants, indicating that SlDnaJ20 overexpression contributes to HSF expression. The yeast two-hybrid system proved that SlDnaJ20 can interact with the chloroplast heat-shock protein 70. Our results indicate that SlDnaJ20 overexpression enhances the thermotolerance of transgenic tomatoes, whereas suppression of SlDnaJ20 increases the heat sensitivity of transgenic tomatoes.http://www.mdpi.com/1422-0067/20/2/367DnaJheat stressHSP70SlDnaJ20tomato |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guodong Wang Guohua Cai Na Xu Litao Zhang Xiuling Sun Jing Guan Qingwei Meng |
spellingShingle |
Guodong Wang Guohua Cai Na Xu Litao Zhang Xiuling Sun Jing Guan Qingwei Meng Novel DnaJ Protein Facilitates Thermotolerance of Transgenic Tomatoes International Journal of Molecular Sciences DnaJ heat stress HSP70 SlDnaJ20 tomato |
author_facet |
Guodong Wang Guohua Cai Na Xu Litao Zhang Xiuling Sun Jing Guan Qingwei Meng |
author_sort |
Guodong Wang |
title |
Novel DnaJ Protein Facilitates Thermotolerance of Transgenic Tomatoes |
title_short |
Novel DnaJ Protein Facilitates Thermotolerance of Transgenic Tomatoes |
title_full |
Novel DnaJ Protein Facilitates Thermotolerance of Transgenic Tomatoes |
title_fullStr |
Novel DnaJ Protein Facilitates Thermotolerance of Transgenic Tomatoes |
title_full_unstemmed |
Novel DnaJ Protein Facilitates Thermotolerance of Transgenic Tomatoes |
title_sort |
novel dnaj protein facilitates thermotolerance of transgenic tomatoes |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-01-01 |
description |
DnaJ proteins, which are molecular chaperones that are widely present in plants, can respond to various environmental stresses. At present, the function of DnaJ proteins was studied in many plant species, but only a few studies were conducted in tomato. Here, we examined the functions of a novel tomato (Solanum lycopersicum) DnaJ protein (SlDnaJ20) in heat tolerance using sense and antisense transgenic tomatoes. Transient conversion assays of Arabidopsis protoplasts showed that SlDnaJ20 was targeted to chloroplasts. Expression analysis showed that SlDnaJ20 expression was induced by chilling, NaCl, polyethylene glycol, and H2O2, especially via heat stress. Under heat stress, sense plants showed higher fresh weights, chlorophyll content, fluorescence (Fv/Fm), and D1 protein levels, and a lower accumulation of reactive oxygen species (ROS) than antisense plants. These results suggest that SlDnaJ20 overexpression can reduce the photoinhibition of photosystem II (PSII) by relieving ROS accumulation. Moreover, higher expression levels of HsfA1 and HsfB1 were observed under heat stress in sense plants, indicating that SlDnaJ20 overexpression contributes to HSF expression. The yeast two-hybrid system proved that SlDnaJ20 can interact with the chloroplast heat-shock protein 70. Our results indicate that SlDnaJ20 overexpression enhances the thermotolerance of transgenic tomatoes, whereas suppression of SlDnaJ20 increases the heat sensitivity of transgenic tomatoes. |
topic |
DnaJ heat stress HSP70 SlDnaJ20 tomato |
url |
http://www.mdpi.com/1422-0067/20/2/367 |
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