Control potential of Meloidogyne javanica and Ditylenchus spp. using fluorescent Pseudomonas and Bacillus spp.
ABSTRACT Plant Growth Promoting Rhizobacteria (PGPR) have different mechanisms of action in the development of plants, such as growth promotion, production of phytohormones and antibiotic substances and changes in root exudates. These help to control plant diseases. In order to evaluate the potentia...
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doaj-e587159aecff4f86bcee38ddbd83c64a2020-11-24T20:53:36ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1678-4405491545910.1016/j.bjm.2017.03.015S1517-83822018000100054Control potential of Meloidogyne javanica and Ditylenchus spp. using fluorescent Pseudomonas and Bacillus spp.Marcielly F. TurattoFernanda dos S. DouradoJerri E. ZilliGlória R. BotelhoABSTRACT Plant Growth Promoting Rhizobacteria (PGPR) have different mechanisms of action in the development of plants, such as growth promotion, production of phytohormones and antibiotic substances and changes in root exudates. These help to control plant diseases. In order to evaluate the potential of microorganisms in the control of Meloidogyne javanica and Ditylenchus spp., five rhizobacteria isolated from rhizosphere of garlic cultivated in the Curitibanos (SC) region were tested. Hatching chambers were set on Petri dishes, in which were added 10 mL of bacterial suspension and 1 mL of M. javanica eggs suspension, at the rate of 4500, on the filter paper of each chamber. The same procedure was performed with 300 juvenile Ditylenchus spp. The experimental design was completely randomized, with four replications. The evaluations were performed every 72 h for nine days. The antagonized population of nematodes was determined in Peters counting chamber, determining the percentage hatching (for M. javanica) and motility (for Ditylenchus spp). Isolates CBSAL02 and CBSAL05 significantly reduced the hatching of M. javanica eggs (74% and 54.77%, respectively) and the motility of Ditylenchus spp. (55.19% and 53.53%, respectively) in vitro. Isolates were identified as belonging to the genera Pseudomonas (CBSAL05) and Bacillus (CBSAL02).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100054&lng=en&tlng=enAllium sativum L.Glycine max (L.) MerrilBiocontrolPlant parasitic nematodesRhizobacteria |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marcielly F. Turatto Fernanda dos S. Dourado Jerri E. Zilli Glória R. Botelho |
spellingShingle |
Marcielly F. Turatto Fernanda dos S. Dourado Jerri E. Zilli Glória R. Botelho Control potential of Meloidogyne javanica and Ditylenchus spp. using fluorescent Pseudomonas and Bacillus spp. Brazilian Journal of Microbiology Allium sativum L. Glycine max (L.) Merril Biocontrol Plant parasitic nematodes Rhizobacteria |
author_facet |
Marcielly F. Turatto Fernanda dos S. Dourado Jerri E. Zilli Glória R. Botelho |
author_sort |
Marcielly F. Turatto |
title |
Control potential of Meloidogyne javanica and Ditylenchus spp. using fluorescent Pseudomonas and Bacillus spp. |
title_short |
Control potential of Meloidogyne javanica and Ditylenchus spp. using fluorescent Pseudomonas and Bacillus spp. |
title_full |
Control potential of Meloidogyne javanica and Ditylenchus spp. using fluorescent Pseudomonas and Bacillus spp. |
title_fullStr |
Control potential of Meloidogyne javanica and Ditylenchus spp. using fluorescent Pseudomonas and Bacillus spp. |
title_full_unstemmed |
Control potential of Meloidogyne javanica and Ditylenchus spp. using fluorescent Pseudomonas and Bacillus spp. |
title_sort |
control potential of meloidogyne javanica and ditylenchus spp. using fluorescent pseudomonas and bacillus spp. |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1678-4405 |
description |
ABSTRACT Plant Growth Promoting Rhizobacteria (PGPR) have different mechanisms of action in the development of plants, such as growth promotion, production of phytohormones and antibiotic substances and changes in root exudates. These help to control plant diseases. In order to evaluate the potential of microorganisms in the control of Meloidogyne javanica and Ditylenchus spp., five rhizobacteria isolated from rhizosphere of garlic cultivated in the Curitibanos (SC) region were tested. Hatching chambers were set on Petri dishes, in which were added 10 mL of bacterial suspension and 1 mL of M. javanica eggs suspension, at the rate of 4500, on the filter paper of each chamber. The same procedure was performed with 300 juvenile Ditylenchus spp. The experimental design was completely randomized, with four replications. The evaluations were performed every 72 h for nine days. The antagonized population of nematodes was determined in Peters counting chamber, determining the percentage hatching (for M. javanica) and motility (for Ditylenchus spp). Isolates CBSAL02 and CBSAL05 significantly reduced the hatching of M. javanica eggs (74% and 54.77%, respectively) and the motility of Ditylenchus spp. (55.19% and 53.53%, respectively) in vitro. Isolates were identified as belonging to the genera Pseudomonas (CBSAL05) and Bacillus (CBSAL02). |
topic |
Allium sativum L. Glycine max (L.) Merril Biocontrol Plant parasitic nematodes Rhizobacteria |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100054&lng=en&tlng=en |
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