A collection of three integration-free iPSCs derived from old male and female healthy subjects

A collection of three integration-free iPSCs derived from old male and female healthy subjects

Here, we present the characterization of three iPSC lines derived from dermal fibroblasts of old healthy subjects. Fibroblasts were reprogrammed using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could generate the three germ layers (...

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Bibliographic Details
Main Authors: Eva Rodríguez-Traver, Eva Díaz-Guerra, César Rodríguez, Fabián Arenas, María Orera, Jaime Kulisevsky, Rosario Moratalla, Carlos Vicario
Format: Article
Language:English
Published: Elsevier 2020-01-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506119302934
Description
Summary:Here, we present the characterization of three iPSC lines derived from dermal fibroblasts of old healthy subjects. Fibroblasts were reprogrammed using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could generate the three germ layers (ectoderm, mesoderm and endoderm), maintained a stable karyotype, and were free from Sendai vectors and reprogramming factors. These integration-free iPSCs can serve for establishing control cell cultures in studies searching for phenotypes and mechanisms that could potentially be dysregulated in degenerative diseases.
ISSN:1873-5061

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