A collection of three integration-free iPSCs derived from old male and female healthy subjects

Here, we present the characterization of three iPSC lines derived from dermal fibroblasts of old healthy subjects. Fibroblasts were reprogrammed using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could generate the three germ layers (...

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Main Authors:	Eva Rodríguez-Traver, Eva Díaz-Guerra, César Rodríguez, Fabián Arenas, María Orera, Jaime Kulisevsky, Rosario Moratalla, Carlos Vicario
Format:	Article
Language:	English
Published:	Elsevier 2020-01-01
Series:	Stem Cell Research
Online Access:	http://www.sciencedirect.com/science/article/pii/S1873506119302934

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spelling	doaj-e58cf9e4b1094feba1c6a7e26ec7c8492020-11-25T00:51:23ZengElsevierStem Cell Research1873-50612020-01-0142A collection of three integration-free iPSCs derived from old male and female healthy subjectsEva Rodríguez-Traver0Eva Díaz-Guerra1César Rodríguez2Fabián Arenas3María Orera4Jaime Kulisevsky5Rosario Moratalla6Carlos Vicario7Instituto Cajal-Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain; Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Madrid, SpainInstituto Cajal-Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain; Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Madrid, SpainServicio de Bioquímica Clínica, Hospital General Universitario Gregorio Marañón, Madrid, SpainCentro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Madrid, Spain; Movement Disorders Unit, Neurology Dept., Hospital Sant Pau, Barcelona, SpainServicio de Bioquímica Clínica, Hospital General Universitario Gregorio Marañón, Madrid, SpainCentro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Madrid, Spain; Movement Disorders Unit, Neurology Dept., Hospital Sant Pau, Barcelona, SpainInstituto Cajal-Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain; Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Madrid, SpainInstituto Cajal-Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain; Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Madrid, Spain; Corresponding author at: Instituto Cajal-Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain.Here, we present the characterization of three iPSC lines derived from dermal fibroblasts of old healthy subjects. Fibroblasts were reprogrammed using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could generate the three germ layers (ectoderm, mesoderm and endoderm), maintained a stable karyotype, and were free from Sendai vectors and reprogramming factors. These integration-free iPSCs can serve for establishing control cell cultures in studies searching for phenotypes and mechanisms that could potentially be dysregulated in degenerative diseases.http://www.sciencedirect.com/science/article/pii/S1873506119302934
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Eva Rodríguez-Traver Eva Díaz-Guerra César Rodríguez Fabián Arenas María Orera Jaime Kulisevsky Rosario Moratalla Carlos Vicario
spellingShingle	Eva Rodríguez-Traver Eva Díaz-Guerra César Rodríguez Fabián Arenas María Orera Jaime Kulisevsky Rosario Moratalla Carlos Vicario A collection of three integration-free iPSCs derived from old male and female healthy subjects Stem Cell Research
author_facet	Eva Rodríguez-Traver Eva Díaz-Guerra César Rodríguez Fabián Arenas María Orera Jaime Kulisevsky Rosario Moratalla Carlos Vicario
author_sort	Eva Rodríguez-Traver
title	A collection of three integration-free iPSCs derived from old male and female healthy subjects
title_short	A collection of three integration-free iPSCs derived from old male and female healthy subjects
title_full	A collection of three integration-free iPSCs derived from old male and female healthy subjects
title_fullStr	A collection of three integration-free iPSCs derived from old male and female healthy subjects
title_full_unstemmed	A collection of three integration-free iPSCs derived from old male and female healthy subjects
title_sort	collection of three integration-free ipscs derived from old male and female healthy subjects
publisher	Elsevier
series	Stem Cell Research
issn	1873-5061
publishDate	2020-01-01
description	Here, we present the characterization of three iPSC lines derived from dermal fibroblasts of old healthy subjects. Fibroblasts were reprogrammed using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could generate the three germ layers (ectoderm, mesoderm and endoderm), maintained a stable karyotype, and were free from Sendai vectors and reprogramming factors. These integration-free iPSCs can serve for establishing control cell cultures in studies searching for phenotypes and mechanisms that could potentially be dysregulated in degenerative diseases.
url	http://www.sciencedirect.com/science/article/pii/S1873506119302934
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A collection of three integration-free iPSCs derived from old male and female healthy subjects

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