gga-miR-146c Activates TLR6/MyD88/NF-κB Pathway through Targeting MMP16 to Prevent <i>Mycoplasma Gallisepticum</i> (HS Strain) Infection in Chickens
<i>Mycoplasma gallisepticum</i> (<i>MG</i>), a pathogen that infects chickens and some other birds, triggers chronic respiratory disease (CRD) in chickens, which is characterized by inflammation. The investigation of microbial pathogenesis would contribute to the deep underst...
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doaj-e5968f8203e14a36bd53d3739de657a32020-11-25T00:52:40ZengMDPI AGCells2073-44092019-05-018550110.3390/cells8050501cells8050501gga-miR-146c Activates TLR6/MyD88/NF-κB Pathway through Targeting MMP16 to Prevent <i>Mycoplasma Gallisepticum</i> (HS Strain) Infection in ChickensKang Zhang0Yun Han1Zaiwei Wang2Yabo Zhao3Yali Fu4Xiuli Peng5Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction Ministry of Education, College of Animal science and Technology and College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction Ministry of Education, College of Animal science and Technology and College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction Ministry of Education, College of Animal science and Technology and College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction Ministry of Education, College of Animal science and Technology and College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction Ministry of Education, College of Animal science and Technology and College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction Ministry of Education, College of Animal science and Technology and College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China<i>Mycoplasma gallisepticum</i> (<i>MG</i>), a pathogen that infects chickens and some other birds, triggers chronic respiratory disease (CRD) in chickens, which is characterized by inflammation. The investigation of microbial pathogenesis would contribute to the deep understanding of infection control. Since microribonucleic acids (miRNAs) play a key role in this process, gga-mir-146c, an upregulated miRNA upon <i>MG</i> infection, was selected according to our previous RNA-sequencing data. In this paper, we predicted and validated that <i>MMP16</i> is one of gga-miR-146c target genes. Results show that <i>MMP16</i> is the target of gga-miR-146c and gga-miR-146c can downregulate <i>MMP16</i> expression within limits. gga-miR-146c upregulation significantly increased the expression of TLR6, NF-κB p65, MyD88, and TNF-α, whereas the gga-miR-146c inhibitor led to an opposite result. gga-miR-146c upregulation effectively decreased apoptosis and stimulated DF-1 cells proliferation upon <i>MG</i> infection. On the contrary, gga-miR-146c inhibitor promoted apoptosis and repressed the proliferation. Collectively, our results suggest that gga-miR-146c upregulation upon MG infection represses <i>MMP16</i> expression, activating TLR6/MyD88/NF-κB pathway, promoting cell proliferation by inhibiting cell apoptosis, and, finally, enhancing cell cycle progression to defend against host <i>MG</i> infection.https://www.mdpi.com/2073-4409/8/5/501<i>Mycoplasma gallisepticum</i>chickenmiR-146cMMP16NF-κB signaling pathway |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kang Zhang Yun Han Zaiwei Wang Yabo Zhao Yali Fu Xiuli Peng |
spellingShingle |
Kang Zhang Yun Han Zaiwei Wang Yabo Zhao Yali Fu Xiuli Peng gga-miR-146c Activates TLR6/MyD88/NF-κB Pathway through Targeting MMP16 to Prevent <i>Mycoplasma Gallisepticum</i> (HS Strain) Infection in Chickens Cells <i>Mycoplasma gallisepticum</i> chicken miR-146c MMP16 NF-κB signaling pathway |
author_facet |
Kang Zhang Yun Han Zaiwei Wang Yabo Zhao Yali Fu Xiuli Peng |
author_sort |
Kang Zhang |
title |
gga-miR-146c Activates TLR6/MyD88/NF-κB Pathway through Targeting MMP16 to Prevent <i>Mycoplasma Gallisepticum</i> (HS Strain) Infection in Chickens |
title_short |
gga-miR-146c Activates TLR6/MyD88/NF-κB Pathway through Targeting MMP16 to Prevent <i>Mycoplasma Gallisepticum</i> (HS Strain) Infection in Chickens |
title_full |
gga-miR-146c Activates TLR6/MyD88/NF-κB Pathway through Targeting MMP16 to Prevent <i>Mycoplasma Gallisepticum</i> (HS Strain) Infection in Chickens |
title_fullStr |
gga-miR-146c Activates TLR6/MyD88/NF-κB Pathway through Targeting MMP16 to Prevent <i>Mycoplasma Gallisepticum</i> (HS Strain) Infection in Chickens |
title_full_unstemmed |
gga-miR-146c Activates TLR6/MyD88/NF-κB Pathway through Targeting MMP16 to Prevent <i>Mycoplasma Gallisepticum</i> (HS Strain) Infection in Chickens |
title_sort |
gga-mir-146c activates tlr6/myd88/nf-κb pathway through targeting mmp16 to prevent <i>mycoplasma gallisepticum</i> (hs strain) infection in chickens |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2019-05-01 |
description |
<i>Mycoplasma gallisepticum</i> (<i>MG</i>), a pathogen that infects chickens and some other birds, triggers chronic respiratory disease (CRD) in chickens, which is characterized by inflammation. The investigation of microbial pathogenesis would contribute to the deep understanding of infection control. Since microribonucleic acids (miRNAs) play a key role in this process, gga-mir-146c, an upregulated miRNA upon <i>MG</i> infection, was selected according to our previous RNA-sequencing data. In this paper, we predicted and validated that <i>MMP16</i> is one of gga-miR-146c target genes. Results show that <i>MMP16</i> is the target of gga-miR-146c and gga-miR-146c can downregulate <i>MMP16</i> expression within limits. gga-miR-146c upregulation significantly increased the expression of TLR6, NF-κB p65, MyD88, and TNF-α, whereas the gga-miR-146c inhibitor led to an opposite result. gga-miR-146c upregulation effectively decreased apoptosis and stimulated DF-1 cells proliferation upon <i>MG</i> infection. On the contrary, gga-miR-146c inhibitor promoted apoptosis and repressed the proliferation. Collectively, our results suggest that gga-miR-146c upregulation upon MG infection represses <i>MMP16</i> expression, activating TLR6/MyD88/NF-κB pathway, promoting cell proliferation by inhibiting cell apoptosis, and, finally, enhancing cell cycle progression to defend against host <i>MG</i> infection. |
topic |
<i>Mycoplasma gallisepticum</i> chicken miR-146c MMP16 NF-κB signaling pathway |
url |
https://www.mdpi.com/2073-4409/8/5/501 |
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