The Effect of Dietary Mushroom <i>Agaricus bisporus</i> on Intestinal Microbiota Composition and Host Immunological Function
A study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function. Thirty-one six-week-old pigs were fed a pig grower diet alone or supplemented with either three or six servings of freeze-dried white...
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doaj-e59b24bbd4b445cca2090ffacd486b882020-11-25T00:37:12ZengMDPI AGNutrients2072-66432018-11-011011172110.3390/nu10111721nu10111721The Effect of Dietary Mushroom <i>Agaricus bisporus</i> on Intestinal Microbiota Composition and Host Immunological FunctionGloria I. Solano-Aguilar0Saebyeol Jang1Sukla Lakshman2Richi Gupta3Ethiopia Beshah4Masoumeh Sikaroodi5Bryan Vinyard6Aleksey Molokin7Patrick M. Gillevet8Joseph F. Urban9Diet Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture Northeast Area, Beltsville, MD 20705, USADiet Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture Northeast Area, Beltsville, MD 20705, USADiet Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture Northeast Area, Beltsville, MD 20705, USAMicrobiome Analysis Center, George Mason University, Science & Technology Campus, Manassas, VA 20108, USADiet Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture Northeast Area, Beltsville, MD 20705, USAMicrobiome Analysis Center, George Mason University, Science & Technology Campus, Manassas, VA 20108, USAStatistics Group, Agricultural Research Service, U.S. Department of Agriculture Northeast Area, Beltsville, MD 20705, USADiet Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture Northeast Area, Beltsville, MD 20705, USAMicrobiome Analysis Center, George Mason University, Science & Technology Campus, Manassas, VA 20108, USADiet Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture Northeast Area, Beltsville, MD 20705, USAA study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function. Thirty-one six-week-old pigs were fed a pig grower diet alone or supplemented with either three or six servings of freeze-dried white button (WB)-mushrooms for six weeks. Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with <i>Salmonella typhymurium</i>-Lipopolysaccharide (LPS). Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function. Pigs gained weight with no difference in body composition or intestinal permeability. Feeding mushrooms reduced LPS-induced <i>IL-1β</i> gene expression in AM (<i>P</i> < 0.05) with no change in LPS-stimulated PBMC or the intestinal mucosa transcriptome. LEfSe indicated increases in <i>Lachnospiraceae</i>, <i>Ruminococcaceae</i> within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs. These results suggested that feeding WB mushrooms significantly reduced the LPS-induced inflammatory response in AM and positively modulated the host microbiota metabolism by increasing the abundance of Clostridiales taxa that are associated with improved intestinal health.https://www.mdpi.com/2072-6643/10/11/1721mushroommicrobiomeimmune responsemacrophage16S rDNA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gloria I. Solano-Aguilar Saebyeol Jang Sukla Lakshman Richi Gupta Ethiopia Beshah Masoumeh Sikaroodi Bryan Vinyard Aleksey Molokin Patrick M. Gillevet Joseph F. Urban |
spellingShingle |
Gloria I. Solano-Aguilar Saebyeol Jang Sukla Lakshman Richi Gupta Ethiopia Beshah Masoumeh Sikaroodi Bryan Vinyard Aleksey Molokin Patrick M. Gillevet Joseph F. Urban The Effect of Dietary Mushroom <i>Agaricus bisporus</i> on Intestinal Microbiota Composition and Host Immunological Function Nutrients mushroom microbiome immune response macrophage 16S rDNA |
author_facet |
Gloria I. Solano-Aguilar Saebyeol Jang Sukla Lakshman Richi Gupta Ethiopia Beshah Masoumeh Sikaroodi Bryan Vinyard Aleksey Molokin Patrick M. Gillevet Joseph F. Urban |
author_sort |
Gloria I. Solano-Aguilar |
title |
The Effect of Dietary Mushroom <i>Agaricus bisporus</i> on Intestinal Microbiota Composition and Host Immunological Function |
title_short |
The Effect of Dietary Mushroom <i>Agaricus bisporus</i> on Intestinal Microbiota Composition and Host Immunological Function |
title_full |
The Effect of Dietary Mushroom <i>Agaricus bisporus</i> on Intestinal Microbiota Composition and Host Immunological Function |
title_fullStr |
The Effect of Dietary Mushroom <i>Agaricus bisporus</i> on Intestinal Microbiota Composition and Host Immunological Function |
title_full_unstemmed |
The Effect of Dietary Mushroom <i>Agaricus bisporus</i> on Intestinal Microbiota Composition and Host Immunological Function |
title_sort |
effect of dietary mushroom <i>agaricus bisporus</i> on intestinal microbiota composition and host immunological function |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2018-11-01 |
description |
A study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function. Thirty-one six-week-old pigs were fed a pig grower diet alone or supplemented with either three or six servings of freeze-dried white button (WB)-mushrooms for six weeks. Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with <i>Salmonella typhymurium</i>-Lipopolysaccharide (LPS). Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function. Pigs gained weight with no difference in body composition or intestinal permeability. Feeding mushrooms reduced LPS-induced <i>IL-1β</i> gene expression in AM (<i>P</i> < 0.05) with no change in LPS-stimulated PBMC or the intestinal mucosa transcriptome. LEfSe indicated increases in <i>Lachnospiraceae</i>, <i>Ruminococcaceae</i> within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs. These results suggested that feeding WB mushrooms significantly reduced the LPS-induced inflammatory response in AM and positively modulated the host microbiota metabolism by increasing the abundance of Clostridiales taxa that are associated with improved intestinal health. |
topic |
mushroom microbiome immune response macrophage 16S rDNA |
url |
https://www.mdpi.com/2072-6643/10/11/1721 |
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