Therapeutic Potential of Vitamin D and Curcumin in an Model of Alzheimer Disease
Background: Alzheimer disease is a progressive neurodegenerative disease, affecting a very high proportion of the aging population. Several studies have demonstrated that one of the main contributors to this disease is oxidative stress (OS), which causes peroxidation of protein, lipids, and DNA resu...
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doaj-e5ac6cf58f7749f6bcd866f4c6decdd52020-11-25T03:36:29ZengSAGE PublishingJournal of Central Nervous System Disease1179-57352020-05-011210.1177/1179573520924311Therapeutic Potential of Vitamin D and Curcumin in an Model of Alzheimer DiseaseAbir Abdullah AlamroEbtesam Atiah AlsulamiMoudhi AlmutlaqAmani AlghamediMajed AlokailSamina Hyder HaqBackground: Alzheimer disease is a progressive neurodegenerative disease, affecting a very high proportion of the aging population. Several studies have demonstrated that one of the main contributors to this disease is oxidative stress (OS), which causes peroxidation of protein, lipids, and DNA resulting in the formation of advanced glycosylated end products (AGE) in the brain tissues. These AGE are usually associated with the amyloid β (Aβ), which could further aggravate its toxicity and its clearance. Antioxidants counteract the deterioration caused by OS. Objective: We aimed to evaluate the effect of vitamin D3 and curcumin on primary cortical neuronal cultures exposed to Aβ 1-42 toxicity for different time periods. Methods: Primary cortical neuronal cultures were set up and exposed to Aβ 1-42 for up to 72 hours. Cell viability was studied by 3[4,5-dimethylthiazole-2-yl]-2,5-dipheyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assay. Biochemical assays for OS such as lipid peroxidation, reduced Glutathione(GSH), Glutathione S-transferase (GST), catalase, and superoxide dismutase (SOD) were conducted. Sandwich enzyme-linked immunosorbent assay (ELISA) was used to study the neurotrophic growth factor (NGF) expression. Results: Treatments with Aβ 1-42 caused an elevation in lipid peroxidation products, which were ameliorated in the presence of vitamin D3 and curcumin. Both enzymatic (GST, catalase, and SOD) and nonenzymatic antioxidants (reduced GSH) were raised significantly in the presence of vitamin D3 and curcumin, which resulted in the better recovery of neuronal cells from Aβ 1-42 treatment. Treatment with vitamin D3 and curcumin also resulted in the upregulation of NGF levels. Conclusions: This study suggests that vitamin D3 and curcumin can be a promising natural therapy for the treatment of Alzheimer disease.https://doi.org/10.1177/1179573520924311 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Abir Abdullah Alamro Ebtesam Atiah Alsulami Moudhi Almutlaq Amani Alghamedi Majed Alokail Samina Hyder Haq |
spellingShingle |
Abir Abdullah Alamro Ebtesam Atiah Alsulami Moudhi Almutlaq Amani Alghamedi Majed Alokail Samina Hyder Haq Therapeutic Potential of Vitamin D and Curcumin in an Model of Alzheimer Disease Journal of Central Nervous System Disease |
author_facet |
Abir Abdullah Alamro Ebtesam Atiah Alsulami Moudhi Almutlaq Amani Alghamedi Majed Alokail Samina Hyder Haq |
author_sort |
Abir Abdullah Alamro |
title |
Therapeutic Potential of Vitamin D and Curcumin in an Model of Alzheimer Disease |
title_short |
Therapeutic Potential of Vitamin D and Curcumin in an Model of Alzheimer Disease |
title_full |
Therapeutic Potential of Vitamin D and Curcumin in an Model of Alzheimer Disease |
title_fullStr |
Therapeutic Potential of Vitamin D and Curcumin in an Model of Alzheimer Disease |
title_full_unstemmed |
Therapeutic Potential of Vitamin D and Curcumin in an Model of Alzheimer Disease |
title_sort |
therapeutic potential of vitamin d and curcumin in an model of alzheimer disease |
publisher |
SAGE Publishing |
series |
Journal of Central Nervous System Disease |
issn |
1179-5735 |
publishDate |
2020-05-01 |
description |
Background: Alzheimer disease is a progressive neurodegenerative disease, affecting a very high proportion of the aging population. Several studies have demonstrated that one of the main contributors to this disease is oxidative stress (OS), which causes peroxidation of protein, lipids, and DNA resulting in the formation of advanced glycosylated end products (AGE) in the brain tissues. These AGE are usually associated with the amyloid β (Aβ), which could further aggravate its toxicity and its clearance. Antioxidants counteract the deterioration caused by OS. Objective: We aimed to evaluate the effect of vitamin D3 and curcumin on primary cortical neuronal cultures exposed to Aβ 1-42 toxicity for different time periods. Methods: Primary cortical neuronal cultures were set up and exposed to Aβ 1-42 for up to 72 hours. Cell viability was studied by 3[4,5-dimethylthiazole-2-yl]-2,5-dipheyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assay. Biochemical assays for OS such as lipid peroxidation, reduced Glutathione(GSH), Glutathione S-transferase (GST), catalase, and superoxide dismutase (SOD) were conducted. Sandwich enzyme-linked immunosorbent assay (ELISA) was used to study the neurotrophic growth factor (NGF) expression. Results: Treatments with Aβ 1-42 caused an elevation in lipid peroxidation products, which were ameliorated in the presence of vitamin D3 and curcumin. Both enzymatic (GST, catalase, and SOD) and nonenzymatic antioxidants (reduced GSH) were raised significantly in the presence of vitamin D3 and curcumin, which resulted in the better recovery of neuronal cells from Aβ 1-42 treatment. Treatment with vitamin D3 and curcumin also resulted in the upregulation of NGF levels. Conclusions: This study suggests that vitamin D3 and curcumin can be a promising natural therapy for the treatment of Alzheimer disease. |
url |
https://doi.org/10.1177/1179573520924311 |
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