An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus
Abstract Background Canine circovirus is a deadly pathogen of dogs and causes vasculitis and hemorrhagic enteritis. It causes lethal gastroenteritis in pigs, fox, and dogs. Canine circovirus genome contains two main (and opposite) transcription units which encode two open reading frames (ORFs), a re...
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doaj-e5b5d09fba614f3ca7b04c52820c5f602021-08-22T11:37:49ZengSpringerOpenJournal of Genetic Engineering and Biotechnology2090-59202021-08-0119111110.1186/s43141-021-00220-4An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirusPankaj Jain0Amit Joshi1Nahid Akhtar2Sunil Krishnan3Vikas Kaushik4Domain of Bioinformatics, School of Bioengineering and Biosciences, Lovely Professional UniversityDomain of Bioinformatics, School of Bioengineering and Biosciences, Lovely Professional UniversityDomain of Bioinformatics, School of Bioengineering and Biosciences, Lovely Professional UniversityDomain of Bioinformatics, School of Bioengineering and Biosciences, Lovely Professional UniversityDomain of Bioinformatics, School of Bioengineering and Biosciences, Lovely Professional UniversityAbstract Background Canine circovirus is a deadly pathogen of dogs and causes vasculitis and hemorrhagic enteritis. It causes lethal gastroenteritis in pigs, fox, and dogs. Canine circovirus genome contains two main (and opposite) transcription units which encode two open reading frames (ORFs), a replicase-associated protein (Rep) and the capsid (Cap) protein. The replicase protein and capsid protein consist of 303 amino acids and 270 amino acids respectively. Several immuno-informatics methods such as epitope screening, molecular docking, and molecular-dynamics simulations were used to craft peptide-based vaccine construct against canine circovirus. Results The vaccine construct was designed by joining the selected epitopes with adjuvants by suitable linker. The cloning and expression of the vaccine construct was also performed using in silico methods. Screening of epitopes was conducted by NetMHC server that uses ANN (Artificial neural networking) algorithm. These methods are fast and cost-effective for screening epitopes that can interact with dog leukocyte antigens (DLA) and initiate an immune response. Overall, 5 epitopes, YQHLPPFRF, YIRAKWINW, ALYRRLTLI, HLQGFVNLK, and GTMNFVARR, were selected and used to design a vaccine construct. The molecular docking and molecular dynamics simulation studies show that these epitopes can bind with DLA molecules with stability. The codon adaptation and in silico cloning studies show that the vaccine can be expressed by Escherichia coli K12 strain. Conclusion The results suggest that the vaccine construct can be useful in preventing the dogs from canine circovirus infections. However, the results need further validation by performing other in vitro and in vivo experiments.https://doi.org/10.1186/s43141-021-00220-4Canine circovirusEpitopeVaccine designingMolecular dockingMolecular dynamic simulationsDog leukocyte antigen |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pankaj Jain Amit Joshi Nahid Akhtar Sunil Krishnan Vikas Kaushik |
spellingShingle |
Pankaj Jain Amit Joshi Nahid Akhtar Sunil Krishnan Vikas Kaushik An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus Journal of Genetic Engineering and Biotechnology Canine circovirus Epitope Vaccine designing Molecular docking Molecular dynamic simulations Dog leukocyte antigen |
author_facet |
Pankaj Jain Amit Joshi Nahid Akhtar Sunil Krishnan Vikas Kaushik |
author_sort |
Pankaj Jain |
title |
An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus |
title_short |
An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus |
title_full |
An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus |
title_fullStr |
An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus |
title_full_unstemmed |
An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus |
title_sort |
immunoinformatics study: designing multivalent t-cell epitope vaccine against canine circovirus |
publisher |
SpringerOpen |
series |
Journal of Genetic Engineering and Biotechnology |
issn |
2090-5920 |
publishDate |
2021-08-01 |
description |
Abstract Background Canine circovirus is a deadly pathogen of dogs and causes vasculitis and hemorrhagic enteritis. It causes lethal gastroenteritis in pigs, fox, and dogs. Canine circovirus genome contains two main (and opposite) transcription units which encode two open reading frames (ORFs), a replicase-associated protein (Rep) and the capsid (Cap) protein. The replicase protein and capsid protein consist of 303 amino acids and 270 amino acids respectively. Several immuno-informatics methods such as epitope screening, molecular docking, and molecular-dynamics simulations were used to craft peptide-based vaccine construct against canine circovirus. Results The vaccine construct was designed by joining the selected epitopes with adjuvants by suitable linker. The cloning and expression of the vaccine construct was also performed using in silico methods. Screening of epitopes was conducted by NetMHC server that uses ANN (Artificial neural networking) algorithm. These methods are fast and cost-effective for screening epitopes that can interact with dog leukocyte antigens (DLA) and initiate an immune response. Overall, 5 epitopes, YQHLPPFRF, YIRAKWINW, ALYRRLTLI, HLQGFVNLK, and GTMNFVARR, were selected and used to design a vaccine construct. The molecular docking and molecular dynamics simulation studies show that these epitopes can bind with DLA molecules with stability. The codon adaptation and in silico cloning studies show that the vaccine can be expressed by Escherichia coli K12 strain. Conclusion The results suggest that the vaccine construct can be useful in preventing the dogs from canine circovirus infections. However, the results need further validation by performing other in vitro and in vivo experiments. |
topic |
Canine circovirus Epitope Vaccine designing Molecular docking Molecular dynamic simulations Dog leukocyte antigen |
url |
https://doi.org/10.1186/s43141-021-00220-4 |
work_keys_str_mv |
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