Gut butyrate-producing organisms correlate to Placenta Specific 8 protein: Importance to colorectal cancer progression
Tumor metastasis or recurrence often occurs in patients with curative resection of colorectal cancer (CRC). Placental-specific 8 (PLAC8), which has increased expression in stool, may be associated with CRC recurrence. Insights into the role of PLAC8 in CRC recurrence and its clinical significance ma...
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doaj-e5b647c0a5d846e1aa6efca5646f1de72020-11-25T00:29:27ZengElsevierJournal of Advanced Research2090-12322020-03-0122720Gut butyrate-producing organisms correlate to Placenta Specific 8 protein: Importance to colorectal cancer progressionChi-Cheng Huang0Ming-Hung Shen1Shao-Kuan Chen2Shung-Haur Yang3Chih-Yi Liu4Jiun-Wen Guo5Kang-Wei Chang6Chi-Jung Huang7Department of Surgery, Taipei-Veterans General Hospital, Taipei, TaiwanDepartment of Surgery, Fu Jen Catholic University Hospital, New Taipei, Taiwan; School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan; Program in Nutrition and Food Science, Fu Jen Catholic University, New Taipei, TaiwanSchool of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan; Department of Urology, Sijhih Cathay General Hospital, New Taipei, TaiwanDepartment of Surgery, Taipei-Veterans General Hospital, Taipei, Taiwan; School of Medicine, College of Medicine, National Yang Ming University, Taipei, Taiwan; Department of Surgery, National Yang-Ming University Hospital, Yilan, TaiwanDepartment of Pathology, Sijhih Cathay General Hospital, New Taipei, TaiwanProgram in Pharmaceutical Biotechnology, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan; Department of Medical Research, Cathay General Hospital, Taipei, TaiwanIsotope Application Division, Institute of Nuclear Energy Research, Taoyuan 32546, Taiwan; Laboratory Animal Center, Taipei Medical University, Taipei 11031, TaiwanSchool of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan; Program in Pharmaceutical Biotechnology, College of Medicine, Fu Jen Catholic University, New Taipei, Taiwan; Department of Medical Research, Cathay General Hospital, Taipei, Taiwan; Department of Biochemistry, National Defense Medical Center, Taipei 11490, Taiwan; Corresponding author at: Department of Medical Research, Cathay General Hospital, Taipei, Taiwan.Tumor metastasis or recurrence often occurs in patients with curative resection of colorectal cancer (CRC). Placental-specific 8 (PLAC8), which has increased expression in stool, may be associated with CRC recurrence. Insights into the role of PLAC8 in CRC recurrence and its clinical significance may support to develop strategies for preventing CRC recurrence and deterioration. Clinical tissues, cell and animal models were used to clarify the roles of PLAC8 in CRC tumorigenesis, invasion, and migration. Next-generation sequencing of 16S ribosomal DNA has been used to assess the gut microbiota in stool of CRC patients. We found that PLAC8 was upregulated in tissues from patients with late-stage CRC. In our in vitro studies, PLAC8 was dynamically regulated in mitotic cells. Overexpressed PLAC8 was nucleated at the centrosome during mitosis, and therefore, PLAC8 overexpression might increase cell growth and migration (all p < 0.05). The tumorigenic and invasive effects of PLAC8 on CRC cells were also confirmed in a xenograft mouse model. We further identified reduced levels of two butyrate-producing organisms, Butyricicoccus and Prevotella spp., in stools from CRC patients. We found that butyrate downregulated PLAC8 expression and induced apoptosis in PLAC8-overexpressing cells. Our data suggests that PLAC8 gene and protein expression and dysbiosis of gut microflora, especially in butyrate-producing microorganisms, may be indicators of CRC progression. Keywords: Colorectal cancer progression, Gut microbiota, Placenta Specific 8, Butyricicoccus, Butyratehttp://www.sciencedirect.com/science/article/pii/S2090123219301833 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chi-Cheng Huang Ming-Hung Shen Shao-Kuan Chen Shung-Haur Yang Chih-Yi Liu Jiun-Wen Guo Kang-Wei Chang Chi-Jung Huang |
spellingShingle |
Chi-Cheng Huang Ming-Hung Shen Shao-Kuan Chen Shung-Haur Yang Chih-Yi Liu Jiun-Wen Guo Kang-Wei Chang Chi-Jung Huang Gut butyrate-producing organisms correlate to Placenta Specific 8 protein: Importance to colorectal cancer progression Journal of Advanced Research |
author_facet |
Chi-Cheng Huang Ming-Hung Shen Shao-Kuan Chen Shung-Haur Yang Chih-Yi Liu Jiun-Wen Guo Kang-Wei Chang Chi-Jung Huang |
author_sort |
Chi-Cheng Huang |
title |
Gut butyrate-producing organisms correlate to Placenta Specific 8 protein: Importance to colorectal cancer progression |
title_short |
Gut butyrate-producing organisms correlate to Placenta Specific 8 protein: Importance to colorectal cancer progression |
title_full |
Gut butyrate-producing organisms correlate to Placenta Specific 8 protein: Importance to colorectal cancer progression |
title_fullStr |
Gut butyrate-producing organisms correlate to Placenta Specific 8 protein: Importance to colorectal cancer progression |
title_full_unstemmed |
Gut butyrate-producing organisms correlate to Placenta Specific 8 protein: Importance to colorectal cancer progression |
title_sort |
gut butyrate-producing organisms correlate to placenta specific 8 protein: importance to colorectal cancer progression |
publisher |
Elsevier |
series |
Journal of Advanced Research |
issn |
2090-1232 |
publishDate |
2020-03-01 |
description |
Tumor metastasis or recurrence often occurs in patients with curative resection of colorectal cancer (CRC). Placental-specific 8 (PLAC8), which has increased expression in stool, may be associated with CRC recurrence. Insights into the role of PLAC8 in CRC recurrence and its clinical significance may support to develop strategies for preventing CRC recurrence and deterioration. Clinical tissues, cell and animal models were used to clarify the roles of PLAC8 in CRC tumorigenesis, invasion, and migration. Next-generation sequencing of 16S ribosomal DNA has been used to assess the gut microbiota in stool of CRC patients. We found that PLAC8 was upregulated in tissues from patients with late-stage CRC. In our in vitro studies, PLAC8 was dynamically regulated in mitotic cells. Overexpressed PLAC8 was nucleated at the centrosome during mitosis, and therefore, PLAC8 overexpression might increase cell growth and migration (all p < 0.05). The tumorigenic and invasive effects of PLAC8 on CRC cells were also confirmed in a xenograft mouse model. We further identified reduced levels of two butyrate-producing organisms, Butyricicoccus and Prevotella spp., in stools from CRC patients. We found that butyrate downregulated PLAC8 expression and induced apoptosis in PLAC8-overexpressing cells. Our data suggests that PLAC8 gene and protein expression and dysbiosis of gut microflora, especially in butyrate-producing microorganisms, may be indicators of CRC progression. Keywords: Colorectal cancer progression, Gut microbiota, Placenta Specific 8, Butyricicoccus, Butyrate |
url |
http://www.sciencedirect.com/science/article/pii/S2090123219301833 |
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