Effect of Different rhBMP-2 and TG-VEGF Ratios on the Formation of Heterotopic Bone and Neovessels
Bioengineered bone substitutes might represent alternatives to autologous bone grafts in medically compromised patients due to reduced operation time and comorbidity. Due to the lack of an inherent vascular system their dimension is limited to the size of critical bone size defect. To overcome this...
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doaj-e5c095e317d04ec186ecf2e3aa566a782020-11-25T00:37:54ZengHindawi LimitedBioMed Research International2314-61332314-61412014-01-01201410.1155/2014/571510571510Effect of Different rhBMP-2 and TG-VEGF Ratios on the Formation of Heterotopic Bone and NeovesselsWei Xin Cai0Li Wu Zheng1Chun Lei Li2Li Ma3Martin Ehrbar4Franz E. Weber5Roger A. Zwahlen6Discipline of Oral and Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Hong KongDiscipline of Oral Diagnosis and Polyclinics, Faculty of Dentistry, The University of Hong Kong, Hong KongDiscipline of Oral Diagnosis and Polyclinics, Faculty of Dentistry, The University of Hong Kong, Hong KongDiscipline of Oral Rehabilitation, Faculty of Dentistry, The University of Hong Kong, Hong KongDepartment of Obstetrics, University Hospital Zurich, Zurich, SwitzerlandOral Biotechnology and Bioengineering, Division of Cranio-Maxillo-Facial and Oral Surgery, University Hospital Zurich, Zurich, SwitzerlandDiscipline of Oral and Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Hong KongBioengineered bone substitutes might represent alternatives to autologous bone grafts in medically compromised patients due to reduced operation time and comorbidity. Due to the lack of an inherent vascular system their dimension is limited to the size of critical bone size defect. To overcome this shortcoming, the experiment tried to create heterotopic bone around vessels. In vivo, a two-component fibrin and thrombin gel containing recombinant bone morphogenic protein (rhBMP-2) and transglutamate vascular endothelial growth factor (TG-VEGF) in different ratios, respectively, was injected into a dimensionally stable membrane tube, wrapped around the femoral vessel bundle in twelve New Zealand white rabbits. Sacrifice occurred eight weeks postoperatively. Microcomputed tomography of the specimens showed significantly increased bone volume in the rhBMP-2 to TG-VEGF ratio of 10 to 1 group. Histology showed new bone formation in close proximity to the vessel bundle. Immunohistochemistry detected increased angiogenesis within the newly formed bone in the rhBMP-2 to TG-VEGF ratios of 3 to 1 and 5 to 1. Heterotopic bone was engineered in vivo around vessels using different rhBMP-2 and TG-VEGF ratios in a fibrin matrix injected into a dimensionally stable membrane tube which prevented direct contact with skeletal muscles.http://dx.doi.org/10.1155/2014/571510 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei Xin Cai Li Wu Zheng Chun Lei Li Li Ma Martin Ehrbar Franz E. Weber Roger A. Zwahlen |
spellingShingle |
Wei Xin Cai Li Wu Zheng Chun Lei Li Li Ma Martin Ehrbar Franz E. Weber Roger A. Zwahlen Effect of Different rhBMP-2 and TG-VEGF Ratios on the Formation of Heterotopic Bone and Neovessels BioMed Research International |
author_facet |
Wei Xin Cai Li Wu Zheng Chun Lei Li Li Ma Martin Ehrbar Franz E. Weber Roger A. Zwahlen |
author_sort |
Wei Xin Cai |
title |
Effect of Different rhBMP-2 and TG-VEGF Ratios on the Formation of Heterotopic Bone and Neovessels |
title_short |
Effect of Different rhBMP-2 and TG-VEGF Ratios on the Formation of Heterotopic Bone and Neovessels |
title_full |
Effect of Different rhBMP-2 and TG-VEGF Ratios on the Formation of Heterotopic Bone and Neovessels |
title_fullStr |
Effect of Different rhBMP-2 and TG-VEGF Ratios on the Formation of Heterotopic Bone and Neovessels |
title_full_unstemmed |
Effect of Different rhBMP-2 and TG-VEGF Ratios on the Formation of Heterotopic Bone and Neovessels |
title_sort |
effect of different rhbmp-2 and tg-vegf ratios on the formation of heterotopic bone and neovessels |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2014-01-01 |
description |
Bioengineered bone substitutes might represent alternatives to autologous bone grafts in medically compromised patients due to reduced operation time and comorbidity. Due to the lack of an inherent vascular system their dimension is limited to the size of critical bone size defect. To overcome this shortcoming, the experiment tried to create heterotopic bone around vessels. In vivo, a two-component fibrin and thrombin gel containing recombinant bone morphogenic protein (rhBMP-2) and transglutamate vascular endothelial growth factor (TG-VEGF) in different ratios, respectively, was injected into a dimensionally stable membrane tube, wrapped around the femoral vessel bundle in twelve New Zealand white rabbits. Sacrifice occurred eight weeks postoperatively. Microcomputed tomography of the specimens showed significantly increased bone volume in the rhBMP-2 to TG-VEGF ratio of 10 to 1 group. Histology showed new bone formation in close proximity to the vessel bundle. Immunohistochemistry detected increased angiogenesis within the newly formed bone in the rhBMP-2 to TG-VEGF ratios of 3 to 1 and 5 to 1. Heterotopic bone was engineered in vivo around vessels using different rhBMP-2 and TG-VEGF ratios in a fibrin matrix injected into a dimensionally stable membrane tube which prevented direct contact with skeletal muscles. |
url |
http://dx.doi.org/10.1155/2014/571510 |
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