Serum Anti-Mullerian Hormone and Estradiol Concentrations in Gilts and Their Age at Puberty
For experiment one, blood samples were obtained from 200 gilts at 90, 120, 150, 180, and 200 days of age. Serum samples from the 30 youngest (166.1 ± 0.7 days) and 30 oldest (198.8 ± 0.6 days) gilts exhibiting estrus by 200 days, and a further 18 gilts that remained anestrus at 200 days, were assaye...
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doaj-e5c349a1653643afa7b7e70c241fa2ad2020-11-25T04:11:46ZengMDPI AGAnimals2076-26152020-11-01102189218910.3390/ani10112189Serum Anti-Mullerian Hormone and Estradiol Concentrations in Gilts and Their Age at PubertyNutthee Am-in0Junpen Suwimonteerabutr1Roy N. Kirkwood2Department of Obstetrics, Gynecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Obstetrics, Gynecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandSchool of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, SA 5371, AustraliaFor experiment one, blood samples were obtained from 200 gilts at 90, 120, 150, 180, and 200 days of age. Serum samples from the 30 youngest (166.1 ± 0.7 days) and 30 oldest (198.8 ± 0.6 days) gilts exhibiting estrus by 200 days, and a further 18 gilts that remained anestrus at 200 days, were assayed for serum concentrations of anti-Mullerian hormone (AMH) and estradiol (E2). Gilts younger at puberty had higher (<i>p</i> < 0.05) AMH levels than those older at puberty, and both groups had higher AMH levels than anestrus gilts (<i>p</i> < 0.05). Regardless of age, serum E2 was higher (<i>p</i> < 0.05) in gilts that achieved puberty than in gilts remaining anestrus. At spontaneous pubertal estrus detection, there was no effect of pubertal age on the number of preovulatory ovarian follicles. For experiment two, 152 prepubertal gilts received an intramuscular (IM) injection of 400 IU eCG plus 200 IU hCG and then received fence-line boar contact to detect estrus onset. Serum AMH concentrations were higher (<i>p</i> < 0.05) in the first 25 gilts to exhibit puberty than the last 28 gilts, with the first gilts also having more preovulatory follicles (<i>p</i> < 0.0001). Taken together, these data support an association between serum AMH concentrations and degree of physiological maturity and ovarian follicular development in gilts.https://www.mdpi.com/2076-2615/10/11/2189giltspubertyexogenous gonadotrophinsserum anti-Mullerian hormone |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nutthee Am-in Junpen Suwimonteerabutr Roy N. Kirkwood |
spellingShingle |
Nutthee Am-in Junpen Suwimonteerabutr Roy N. Kirkwood Serum Anti-Mullerian Hormone and Estradiol Concentrations in Gilts and Their Age at Puberty Animals gilts puberty exogenous gonadotrophins serum anti-Mullerian hormone |
author_facet |
Nutthee Am-in Junpen Suwimonteerabutr Roy N. Kirkwood |
author_sort |
Nutthee Am-in |
title |
Serum Anti-Mullerian Hormone and Estradiol Concentrations in Gilts and Their Age at Puberty |
title_short |
Serum Anti-Mullerian Hormone and Estradiol Concentrations in Gilts and Their Age at Puberty |
title_full |
Serum Anti-Mullerian Hormone and Estradiol Concentrations in Gilts and Their Age at Puberty |
title_fullStr |
Serum Anti-Mullerian Hormone and Estradiol Concentrations in Gilts and Their Age at Puberty |
title_full_unstemmed |
Serum Anti-Mullerian Hormone and Estradiol Concentrations in Gilts and Their Age at Puberty |
title_sort |
serum anti-mullerian hormone and estradiol concentrations in gilts and their age at puberty |
publisher |
MDPI AG |
series |
Animals |
issn |
2076-2615 |
publishDate |
2020-11-01 |
description |
For experiment one, blood samples were obtained from 200 gilts at 90, 120, 150, 180, and 200 days of age. Serum samples from the 30 youngest (166.1 ± 0.7 days) and 30 oldest (198.8 ± 0.6 days) gilts exhibiting estrus by 200 days, and a further 18 gilts that remained anestrus at 200 days, were assayed for serum concentrations of anti-Mullerian hormone (AMH) and estradiol (E2). Gilts younger at puberty had higher (<i>p</i> < 0.05) AMH levels than those older at puberty, and both groups had higher AMH levels than anestrus gilts (<i>p</i> < 0.05). Regardless of age, serum E2 was higher (<i>p</i> < 0.05) in gilts that achieved puberty than in gilts remaining anestrus. At spontaneous pubertal estrus detection, there was no effect of pubertal age on the number of preovulatory ovarian follicles. For experiment two, 152 prepubertal gilts received an intramuscular (IM) injection of 400 IU eCG plus 200 IU hCG and then received fence-line boar contact to detect estrus onset. Serum AMH concentrations were higher (<i>p</i> < 0.05) in the first 25 gilts to exhibit puberty than the last 28 gilts, with the first gilts also having more preovulatory follicles (<i>p</i> < 0.0001). Taken together, these data support an association between serum AMH concentrations and degree of physiological maturity and ovarian follicular development in gilts. |
topic |
gilts puberty exogenous gonadotrophins serum anti-Mullerian hormone |
url |
https://www.mdpi.com/2076-2615/10/11/2189 |
work_keys_str_mv |
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