Identification of seed storage proteins as the major constituents of the extra virgin olive oil proteome

Proteins are minor components of extra virgin olive oil (EVOO), but the nature of the olive oil proteome is still elusive. In this paper, we have uncovered the EVOO proteome for the first time. Seed storage proteins of globulin-type were identified as the most abundant proteins in EVOO, which also c...

Full description

Bibliographic Details
Main Authors:	Antonio Jesús Castro, Elena Lima-Cabello, Juan de Dios Alché
Format:	Article
Language:	English
Published:	Elsevier 2020-09-01
Series:	Food Chemistry: X
Subjects:	extra virgin olive oil (EVOO) Lipoxygenase Olea europaea Proteomics seed storage proteins (SSP)
Online Access:	http://www.sciencedirect.com/science/article/pii/S2590157520300237

id	doaj-e5c5c3cc507e4c1e9f2fc0d2094748c8
record_format	Article
spelling	doaj-e5c5c3cc507e4c1e9f2fc0d2094748c82020-11-25T03:13:17ZengElsevierFood Chemistry: X2590-15752020-09-017100099Identification of seed storage proteins as the major constituents of the extra virgin olive oil proteomeAntonio Jesús Castro0Elena Lima-Cabello1Juan de Dios Alché2Plant Reproductive Biology and Advanced Imaging Laboratory, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, 18008 Granada, SpainPlant Reproductive Biology and Advanced Imaging Laboratory, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, 18008 Granada, SpainCorresponding author.; Plant Reproductive Biology and Advanced Imaging Laboratory, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, 18008 Granada, SpainProteins are minor components of extra virgin olive oil (EVOO), but the nature of the olive oil proteome is still elusive. In this paper, we have uncovered the EVOO proteome for the first time. Seed storage proteins of globulin-type were identified as the most abundant proteins in EVOO, which also contains an active 13-lipoxygenase and several potential allergenic proteins, including the “panallergen” profilin. We validated our proteomic data by Western blotting and enzyme activity assays. Our data also demonstrated that the seed is the main source of proteins in EVOO, while the contribution of the pulp is uncertain and needs further verification. The impact of EVOO proteins on its stability and quality, and on human health is discussed.http://www.sciencedirect.com/science/article/pii/S2590157520300237extra virgin olive oil (EVOO)LipoxygenaseOlea europaeaProteomicsseed storage proteins (SSP)
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Antonio Jesús Castro Elena Lima-Cabello Juan de Dios Alché
spellingShingle	Antonio Jesús Castro Elena Lima-Cabello Juan de Dios Alché Identification of seed storage proteins as the major constituents of the extra virgin olive oil proteome Food Chemistry: X extra virgin olive oil (EVOO) Lipoxygenase Olea europaea Proteomics seed storage proteins (SSP)
author_facet	Antonio Jesús Castro Elena Lima-Cabello Juan de Dios Alché
author_sort	Antonio Jesús Castro
title	Identification of seed storage proteins as the major constituents of the extra virgin olive oil proteome
title_short	Identification of seed storage proteins as the major constituents of the extra virgin olive oil proteome
title_full	Identification of seed storage proteins as the major constituents of the extra virgin olive oil proteome
title_fullStr	Identification of seed storage proteins as the major constituents of the extra virgin olive oil proteome
title_full_unstemmed	Identification of seed storage proteins as the major constituents of the extra virgin olive oil proteome
title_sort	identification of seed storage proteins as the major constituents of the extra virgin olive oil proteome
publisher	Elsevier
series	Food Chemistry: X
issn	2590-1575
publishDate	2020-09-01
description	Proteins are minor components of extra virgin olive oil (EVOO), but the nature of the olive oil proteome is still elusive. In this paper, we have uncovered the EVOO proteome for the first time. Seed storage proteins of globulin-type were identified as the most abundant proteins in EVOO, which also contains an active 13-lipoxygenase and several potential allergenic proteins, including the “panallergen” profilin. We validated our proteomic data by Western blotting and enzyme activity assays. Our data also demonstrated that the seed is the main source of proteins in EVOO, while the contribution of the pulp is uncertain and needs further verification. The impact of EVOO proteins on its stability and quality, and on human health is discussed.
topic	extra virgin olive oil (EVOO) Lipoxygenase Olea europaea Proteomics seed storage proteins (SSP)
url	http://www.sciencedirect.com/science/article/pii/S2590157520300237
work_keys_str_mv	AT antoniojesuscastro identificationofseedstorageproteinsasthemajorconstituentsoftheextravirginoliveoilproteome AT elenalimacabello identificationofseedstorageproteinsasthemajorconstituentsoftheextravirginoliveoilproteome AT juandediosalche identificationofseedstorageproteinsasthemajorconstituentsoftheextravirginoliveoilproteome
_version_	1724647671457644544

Identification of seed storage proteins as the major constituents of the extra virgin olive oil proteome

Similar Items