RpoE fine tunes expression of a subset of SsrB-regulated virulence factors in <it>Salmonella enterica </it>serovar Typhimurium
<p>Abstract</p> <p>Background</p> <p>The survival of <it>Salmonella enterica </it>within the intracellular host niche requires highly co-ordinated expression of virulence effectors predominantly regulated by the SsrAB two-component regulatory system. <it&...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2009-02-01
|
Series: | BMC Microbiology |
Online Access: | http://www.biomedcentral.com/1471-2180/9/45 |
id |
doaj-e5cf142f1ff84228a60027776f9a9df8 |
---|---|
record_format |
Article |
spelling |
doaj-e5cf142f1ff84228a60027776f9a9df82020-11-24T21:33:53ZengBMCBMC Microbiology1471-21802009-02-01914510.1186/1471-2180-9-45RpoE fine tunes expression of a subset of SsrB-regulated virulence factors in <it>Salmonella enterica </it>serovar TyphimuriumCoombes Brian KOsborne Suzanne E<p>Abstract</p> <p>Background</p> <p>The survival of <it>Salmonella enterica </it>within the intracellular host niche requires highly co-ordinated expression of virulence effectors predominantly regulated by the SsrAB two-component regulatory system. <it>S. enterica </it>serovar Typhimurium mutants lacking the <it>ssrAB </it>genes are avirulent in mice, highlighting the importance of this regulatory system <it>in vivo</it>. Mutants lacking the gene encoding the alternative sigma factor σ<sup>E </sup>(<it>rpoE</it>) are also highly attenuated for intracellular survival, pointing to a potential connection with the SsrAB regulatory system.</p> <p>Results</p> <p>In this study we demonstrate that RpoE is involved in fine-tuning the expression of a subset of SsrB-regulated genes found in the <it>Salmonella </it>pathogenicity island-2 (SPI-2) genetic locus that encodes a horizontally acquired type III secretion system, and unlinked genes integrated into this regulon that are required for virulence in host animals.</p> <p>Conclusion</p> <p>These data point to a potential connection between the virulence phenotype of strains lacking <it>ssrB </it>and <it>rpoE</it>, and highlight new transcriptional regulation that might be essential for appropriate temporal and spatial control of the virulence-associated type III secretion system during host infection.</p> http://www.biomedcentral.com/1471-2180/9/45 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Coombes Brian K Osborne Suzanne E |
spellingShingle |
Coombes Brian K Osborne Suzanne E RpoE fine tunes expression of a subset of SsrB-regulated virulence factors in <it>Salmonella enterica </it>serovar Typhimurium BMC Microbiology |
author_facet |
Coombes Brian K Osborne Suzanne E |
author_sort |
Coombes Brian K |
title |
RpoE fine tunes expression of a subset of SsrB-regulated virulence factors in <it>Salmonella enterica </it>serovar Typhimurium |
title_short |
RpoE fine tunes expression of a subset of SsrB-regulated virulence factors in <it>Salmonella enterica </it>serovar Typhimurium |
title_full |
RpoE fine tunes expression of a subset of SsrB-regulated virulence factors in <it>Salmonella enterica </it>serovar Typhimurium |
title_fullStr |
RpoE fine tunes expression of a subset of SsrB-regulated virulence factors in <it>Salmonella enterica </it>serovar Typhimurium |
title_full_unstemmed |
RpoE fine tunes expression of a subset of SsrB-regulated virulence factors in <it>Salmonella enterica </it>serovar Typhimurium |
title_sort |
rpoe fine tunes expression of a subset of ssrb-regulated virulence factors in <it>salmonella enterica </it>serovar typhimurium |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2009-02-01 |
description |
<p>Abstract</p> <p>Background</p> <p>The survival of <it>Salmonella enterica </it>within the intracellular host niche requires highly co-ordinated expression of virulence effectors predominantly regulated by the SsrAB two-component regulatory system. <it>S. enterica </it>serovar Typhimurium mutants lacking the <it>ssrAB </it>genes are avirulent in mice, highlighting the importance of this regulatory system <it>in vivo</it>. Mutants lacking the gene encoding the alternative sigma factor σ<sup>E </sup>(<it>rpoE</it>) are also highly attenuated for intracellular survival, pointing to a potential connection with the SsrAB regulatory system.</p> <p>Results</p> <p>In this study we demonstrate that RpoE is involved in fine-tuning the expression of a subset of SsrB-regulated genes found in the <it>Salmonella </it>pathogenicity island-2 (SPI-2) genetic locus that encodes a horizontally acquired type III secretion system, and unlinked genes integrated into this regulon that are required for virulence in host animals.</p> <p>Conclusion</p> <p>These data point to a potential connection between the virulence phenotype of strains lacking <it>ssrB </it>and <it>rpoE</it>, and highlight new transcriptional regulation that might be essential for appropriate temporal and spatial control of the virulence-associated type III secretion system during host infection.</p> |
url |
http://www.biomedcentral.com/1471-2180/9/45 |
work_keys_str_mv |
AT coombesbriank rpoefinetunesexpressionofasubsetofssrbregulatedvirulencefactorsinitsalmonellaentericaitserovartyphimurium AT osbornesuzannee rpoefinetunesexpressionofasubsetofssrbregulatedvirulencefactorsinitsalmonellaentericaitserovartyphimurium |
_version_ |
1725951388413853696 |