Phytochemical Characterization and Chemotherapeutic Potential of Cinnamomum verum Extracts on the Multiplication of Protozoan Parasites In Vitro and In Vivo

Cinnamomum verum is a commonly used herbal plant that has several documented properties against various diseases. The existing study evaluated the inhibitory effect of acetonic extract of C. verum (AECV) and ethyl acetate extract of C. verum (...

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Main Authors:	Gaber El-Saber Batiha, Amany Magdy Beshbishy, Azirwan Guswanto, Arifin Nugraha, Tserendorj Munkhjargal, Mohamed M. Abdel-Daim, Juan Mosqueda, Ikuo Igarashi
Format:	Article
Language:	English
Published:	MDPI AG 2020-02-01
Series:	Molecules
Subjects:	<i>cinnamomum verum</i> antipiroplasm drugs phytochemical estimation bioactive constituents
Online Access:	https://www.mdpi.com/1420-3049/25/4/996

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spelling	doaj-e5d0e1c196af4b8c8304ad77f708a4d62020-11-25T02:16:11ZengMDPI AGMolecules1420-30492020-02-0125499610.3390/molecules25040996molecules25040996Phytochemical Characterization and Chemotherapeutic Potential of <i>Cinnamomum verum</i> Extracts on the Multiplication of Protozoan Parasites In Vitro and In VivoGaber El-Saber Batiha0Amany Magdy Beshbishy1Azirwan Guswanto2Arifin Nugraha3Tserendorj Munkhjargal4Mohamed M. Abdel-Daim5Juan Mosqueda6Ikuo Igarashi7National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-13, Inada-cho, Obihiro, Hokkaido 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-13, Inada-cho, Obihiro, Hokkaido 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-13, Inada-cho, Obihiro, Hokkaido 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-13, Inada-cho, Obihiro, Hokkaido 080-8555, JapanLaboratory of Helminthology, Institute of Veterinary Medicine, Zaisan−17042, Ulaanbaatar, MongoliaDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-13, Inada-cho, Obihiro, Hokkaido 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-13, Inada-cho, Obihiro, Hokkaido 080-8555, Japan<i>Cinnamomum verum</i> is a commonly used herbal plant that has several documented properties against various diseases. The existing study evaluated the inhibitory effect of acetonic extract of <i>C. verum</i> (AECV) and ethyl acetate extract of <i>C. verum</i> (EAECV) against piroplasm parasites in vitro and in vivo. The drug-exposure viability assay was tested on Madin-Darby bovine kidney (MDBK), mouse embryonic fibroblast (NIH/3T3) and human foreskin fibroblast (HFF) cells. Qualitative phytochemical estimation revealed that AECV and EAECV containing multiple bioactive constituents namely alkaloids, tannins, saponins, terpenoids and remarkable amounts of polyphenols and flavonoids. AECV and EAECV inhibited <i>B. bovis</i>, <i>B. bigemina</i>, <i>B. divergens</i>, <i>B. caballi</i>, and <i>T. equi</i> multiplication at half-maximal inhibitory concentrations (IC<sub>50</sub>) of 23.1 ± 1.4, 56.6 ± 9.1, 33.4 ± 2.1, 40.3 ± 7.5, 18.8 ± 1.6 µg/mL, and 40.1 ± 8.5, 55.6 ± 1.1, 45.7 ± 1.9, 50.2 ± 6.2, and 61.5 ± 5.2 µg/mL, respectively. In the cytotoxicity assay, AECV and EAECV affected the viability of MDBK, NIH/3T3 and HFF cells with half-maximum effective concentrations (EC<sub>50</sub>) of 440 ± 10.6, 816 ± 12.7 and 914 ± 12.2 µg/mL and 376 ± 11.2, 610 ± 7.7 and 790 ± 12.4 µg/mL, respectively. The in vivo experiment showed that AECV and EAECV were effective against <i>B. microti</i> in mice at 150 mg/kg. These results showed that <i>C. verum</i> extracts are potential antipiroplasm drugs after further studies in some clinical cases.https://www.mdpi.com/1420-3049/25/4/996<i>cinnamomum verum</i>antipiroplasm drugsphytochemical estimationbioactive constituents
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Gaber El-Saber Batiha Amany Magdy Beshbishy Azirwan Guswanto Arifin Nugraha Tserendorj Munkhjargal Mohamed M. Abdel-Daim Juan Mosqueda Ikuo Igarashi
spellingShingle	Gaber El-Saber Batiha Amany Magdy Beshbishy Azirwan Guswanto Arifin Nugraha Tserendorj Munkhjargal Mohamed M. Abdel-Daim Juan Mosqueda Ikuo Igarashi Phytochemical Characterization and Chemotherapeutic Potential of <i>Cinnamomum verum</i> Extracts on the Multiplication of Protozoan Parasites In Vitro and In Vivo Molecules <i>cinnamomum verum</i> antipiroplasm drugs phytochemical estimation bioactive constituents
author_facet	Gaber El-Saber Batiha Amany Magdy Beshbishy Azirwan Guswanto Arifin Nugraha Tserendorj Munkhjargal Mohamed M. Abdel-Daim Juan Mosqueda Ikuo Igarashi
author_sort	Gaber El-Saber Batiha
title	Phytochemical Characterization and Chemotherapeutic Potential of <i>Cinnamomum verum</i> Extracts on the Multiplication of Protozoan Parasites In Vitro and In Vivo
title_short	Phytochemical Characterization and Chemotherapeutic Potential of <i>Cinnamomum verum</i> Extracts on the Multiplication of Protozoan Parasites In Vitro and In Vivo
title_full	Phytochemical Characterization and Chemotherapeutic Potential of <i>Cinnamomum verum</i> Extracts on the Multiplication of Protozoan Parasites In Vitro and In Vivo
title_fullStr	Phytochemical Characterization and Chemotherapeutic Potential of <i>Cinnamomum verum</i> Extracts on the Multiplication of Protozoan Parasites In Vitro and In Vivo
title_full_unstemmed	Phytochemical Characterization and Chemotherapeutic Potential of <i>Cinnamomum verum</i> Extracts on the Multiplication of Protozoan Parasites In Vitro and In Vivo
title_sort	phytochemical characterization and chemotherapeutic potential of <i>cinnamomum verum</i> extracts on the multiplication of protozoan parasites in vitro and in vivo
publisher	MDPI AG
series	Molecules
issn	1420-3049
publishDate	2020-02-01
description	<i>Cinnamomum verum</i> is a commonly used herbal plant that has several documented properties against various diseases. The existing study evaluated the inhibitory effect of acetonic extract of <i>C. verum</i> (AECV) and ethyl acetate extract of <i>C. verum</i> (EAECV) against piroplasm parasites in vitro and in vivo. The drug-exposure viability assay was tested on Madin-Darby bovine kidney (MDBK), mouse embryonic fibroblast (NIH/3T3) and human foreskin fibroblast (HFF) cells. Qualitative phytochemical estimation revealed that AECV and EAECV containing multiple bioactive constituents namely alkaloids, tannins, saponins, terpenoids and remarkable amounts of polyphenols and flavonoids. AECV and EAECV inhibited <i>B. bovis</i>, <i>B. bigemina</i>, <i>B. divergens</i>, <i>B. caballi</i>, and <i>T. equi</i> multiplication at half-maximal inhibitory concentrations (IC<sub>50</sub>) of 23.1 ± 1.4, 56.6 ± 9.1, 33.4 ± 2.1, 40.3 ± 7.5, 18.8 ± 1.6 µg/mL, and 40.1 ± 8.5, 55.6 ± 1.1, 45.7 ± 1.9, 50.2 ± 6.2, and 61.5 ± 5.2 µg/mL, respectively. In the cytotoxicity assay, AECV and EAECV affected the viability of MDBK, NIH/3T3 and HFF cells with half-maximum effective concentrations (EC<sub>50</sub>) of 440 ± 10.6, 816 ± 12.7 and 914 ± 12.2 µg/mL and 376 ± 11.2, 610 ± 7.7 and 790 ± 12.4 µg/mL, respectively. The in vivo experiment showed that AECV and EAECV were effective against <i>B. microti</i> in mice at 150 mg/kg. These results showed that <i>C. verum</i> extracts are potential antipiroplasm drugs after further studies in some clinical cases.
topic	<i>cinnamomum verum</i> antipiroplasm drugs phytochemical estimation bioactive constituents
url	https://www.mdpi.com/1420-3049/25/4/996
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Phytochemical Characterization and Chemotherapeutic Potential of <i>Cinnamomum verum</i> Extracts on the Multiplication of Protozoan Parasites In Vitro and In Vivo

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