Bacteriophage-Based Biosensing of <i>Pseudomonas aeruginosa</i>: An Integrated Approach for the Putative Real-Time Detection of Multi-Drug-Resistant Strains
During the last decennium, it has become widely accepted that ubiquitous bacterial viruses, or bacteriophages, exert enormous influences on our planet’s biosphere, killing between 4–50% of the daily produced bacteria and constituting the largest genetic diversity pool on our planet. Currently, bacte...
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doaj-e5e20bba38e142da9efc96a037c8e5d52021-04-15T23:05:05ZengMDPI AGBiosensors2079-63742021-04-011112412410.3390/bios11040124Bacteriophage-Based Biosensing of <i>Pseudomonas aeruginosa</i>: An Integrated Approach for the Putative Real-Time Detection of Multi-Drug-Resistant StrainsLiliam K. Harada0Waldemar Bonventi Júnior1Erica C. Silva2Thais J. Oliveira3Fernanda C. Moreli4José M. Oliveira Júnior5Matthieu Tubino6Marta M. D. C. Vila7Victor M. Balcão8PhageLab—Laboratory of Biofilms and Bacteriophages, University of Sorocaba, Sorocaba, SP 18023-000, BrazilFaculty of Technology of Sorocaba—FATEC SO, Sorocaba, SP 18013-280, BrazilPhageLab—Laboratory of Biofilms and Bacteriophages, University of Sorocaba, Sorocaba, SP 18023-000, BrazilPhageLab—Laboratory of Biofilms and Bacteriophages, University of Sorocaba, Sorocaba, SP 18023-000, BrazilPhageLab—Laboratory of Biofilms and Bacteriophages, University of Sorocaba, Sorocaba, SP 18023-000, BrazilPhageLab—Laboratory of Biofilms and Bacteriophages, University of Sorocaba, Sorocaba, SP 18023-000, BrazilInstitute of Chemistry, University of Campinas, Campinas, SP 13083-970, BrazilPhageLab—Laboratory of Biofilms and Bacteriophages, University of Sorocaba, Sorocaba, SP 18023-000, BrazilPhageLab—Laboratory of Biofilms and Bacteriophages, University of Sorocaba, Sorocaba, SP 18023-000, BrazilDuring the last decennium, it has become widely accepted that ubiquitous bacterial viruses, or bacteriophages, exert enormous influences on our planet’s biosphere, killing between 4–50% of the daily produced bacteria and constituting the largest genetic diversity pool on our planet. Currently, bacterial infections linked to healthcare services are widespread, which, when associated with the increasing surge of antibiotic-resistant microorganisms, play a major role in patient morbidity and mortality. In this scenario, <i>Pseudomonas aeruginosa</i> alone is responsible for ca. 13–15% of all hospital-acquired infections. The pathogen <i>P. aeruginosa</i> is an opportunistic one, being endowed with metabolic versatility and high (both intrinsic and acquired) resistance to antibiotics. Bacteriophages (or phages) have been recognized as a tool with high potential for the detection of bacterial infections since these metabolically inert entities specifically attach to, and lyse, bacterial host cells, thus, allowing confirmation of the presence of viable cells. In the research effort described herein, three different phages with broad lytic spectrum capable of infecting <i>P. aeruginosa</i> were isolated from environmental sources. The isolated phages were elected on the basis of their ability to form clear and distinctive plaques, which is a hallmark characteristic of virulent phages. Next, their structural and functional stabilization was achieved via entrapment within the matrix of porous alginate, biopolymeric, and bio-reactive, chromogenic hydrogels aiming at their use as sensitive matrices producing both color changes and/or light emissions evolving from a reaction with (released) cytoplasmic moieties, as a bio-detection kit for <i>P. aeruginosa</i> cells. Full physicochemical and biological characterization of the isolated bacteriophages was the subject of a previous research paper.https://www.mdpi.com/2079-6374/11/4/124bacteriophage particles<i>Pseudomonas aeruginosa</i>immobilization and structural/functional stabilizationbacterial biosensingbio-reactive polymeric matrixchromogenic/bioluminescent bio-hydrogel |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liliam K. Harada Waldemar Bonventi Júnior Erica C. Silva Thais J. Oliveira Fernanda C. Moreli José M. Oliveira Júnior Matthieu Tubino Marta M. D. C. Vila Victor M. Balcão |
spellingShingle |
Liliam K. Harada Waldemar Bonventi Júnior Erica C. Silva Thais J. Oliveira Fernanda C. Moreli José M. Oliveira Júnior Matthieu Tubino Marta M. D. C. Vila Victor M. Balcão Bacteriophage-Based Biosensing of <i>Pseudomonas aeruginosa</i>: An Integrated Approach for the Putative Real-Time Detection of Multi-Drug-Resistant Strains Biosensors bacteriophage particles <i>Pseudomonas aeruginosa</i> immobilization and structural/functional stabilization bacterial biosensing bio-reactive polymeric matrix chromogenic/bioluminescent bio-hydrogel |
author_facet |
Liliam K. Harada Waldemar Bonventi Júnior Erica C. Silva Thais J. Oliveira Fernanda C. Moreli José M. Oliveira Júnior Matthieu Tubino Marta M. D. C. Vila Victor M. Balcão |
author_sort |
Liliam K. Harada |
title |
Bacteriophage-Based Biosensing of <i>Pseudomonas aeruginosa</i>: An Integrated Approach for the Putative Real-Time Detection of Multi-Drug-Resistant Strains |
title_short |
Bacteriophage-Based Biosensing of <i>Pseudomonas aeruginosa</i>: An Integrated Approach for the Putative Real-Time Detection of Multi-Drug-Resistant Strains |
title_full |
Bacteriophage-Based Biosensing of <i>Pseudomonas aeruginosa</i>: An Integrated Approach for the Putative Real-Time Detection of Multi-Drug-Resistant Strains |
title_fullStr |
Bacteriophage-Based Biosensing of <i>Pseudomonas aeruginosa</i>: An Integrated Approach for the Putative Real-Time Detection of Multi-Drug-Resistant Strains |
title_full_unstemmed |
Bacteriophage-Based Biosensing of <i>Pseudomonas aeruginosa</i>: An Integrated Approach for the Putative Real-Time Detection of Multi-Drug-Resistant Strains |
title_sort |
bacteriophage-based biosensing of <i>pseudomonas aeruginosa</i>: an integrated approach for the putative real-time detection of multi-drug-resistant strains |
publisher |
MDPI AG |
series |
Biosensors |
issn |
2079-6374 |
publishDate |
2021-04-01 |
description |
During the last decennium, it has become widely accepted that ubiquitous bacterial viruses, or bacteriophages, exert enormous influences on our planet’s biosphere, killing between 4–50% of the daily produced bacteria and constituting the largest genetic diversity pool on our planet. Currently, bacterial infections linked to healthcare services are widespread, which, when associated with the increasing surge of antibiotic-resistant microorganisms, play a major role in patient morbidity and mortality. In this scenario, <i>Pseudomonas aeruginosa</i> alone is responsible for ca. 13–15% of all hospital-acquired infections. The pathogen <i>P. aeruginosa</i> is an opportunistic one, being endowed with metabolic versatility and high (both intrinsic and acquired) resistance to antibiotics. Bacteriophages (or phages) have been recognized as a tool with high potential for the detection of bacterial infections since these metabolically inert entities specifically attach to, and lyse, bacterial host cells, thus, allowing confirmation of the presence of viable cells. In the research effort described herein, three different phages with broad lytic spectrum capable of infecting <i>P. aeruginosa</i> were isolated from environmental sources. The isolated phages were elected on the basis of their ability to form clear and distinctive plaques, which is a hallmark characteristic of virulent phages. Next, their structural and functional stabilization was achieved via entrapment within the matrix of porous alginate, biopolymeric, and bio-reactive, chromogenic hydrogels aiming at their use as sensitive matrices producing both color changes and/or light emissions evolving from a reaction with (released) cytoplasmic moieties, as a bio-detection kit for <i>P. aeruginosa</i> cells. Full physicochemical and biological characterization of the isolated bacteriophages was the subject of a previous research paper. |
topic |
bacteriophage particles <i>Pseudomonas aeruginosa</i> immobilization and structural/functional stabilization bacterial biosensing bio-reactive polymeric matrix chromogenic/bioluminescent bio-hydrogel |
url |
https://www.mdpi.com/2079-6374/11/4/124 |
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