Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates
51V NMR spectroscopy is used to document, using speciation analysis, that one oxometalate is a more potent growth inhibitor of two Mycobacterial strains than other oxovanadates, thus demonstrating selectivity in its interaction with cells. Historically, oxometalates have had many applications in bio...
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doaj-e60665626d584b98ab5c889000e1d6852020-11-24T21:47:11ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462018-11-01610.3389/fchem.2018.00519409848Decavanadate Inhibits Mycobacterial Growth More Potently Than Other OxovanadatesNuttaporn Samart0Nuttaporn Samart1Zeyad Arhouma2Zeyad Arhouma3Santosh Kumar4Heide A. Murakami5Dean C. Crick6Dean C. Crick7Debbie C. Crans8Debbie C. Crans9Department of Chemistry, Colorado State University, Fort Collins, CO, United StatesDepartment of Chemistry, Rajabhat Rajanagarindra University, Chachoengsao, ThailandDepartment of Chemistry, Colorado State University, Fort Collins, CO, United StatesCell and Molecular Biology Program, Colorado State University, Fort Collins, CO, United StatesDepartment of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, United StatesDepartment of Chemistry, Colorado State University, Fort Collins, CO, United StatesCell and Molecular Biology Program, Colorado State University, Fort Collins, CO, United StatesDepartment of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, United StatesDepartment of Chemistry, Colorado State University, Fort Collins, CO, United StatesCell and Molecular Biology Program, Colorado State University, Fort Collins, CO, United States51V NMR spectroscopy is used to document, using speciation analysis, that one oxometalate is a more potent growth inhibitor of two Mycobacterial strains than other oxovanadates, thus demonstrating selectivity in its interaction with cells. Historically, oxometalates have had many applications in biological and medical studies, including study of the phase-problem in X-ray crystallography of the ribosome. The effect of different vanadate salts on the growth of Mycobacterium smegmatis (M. smeg) and Mycobacterium tuberculosis (M. tb) was investigated, and speciation was found to be critical for the observed growth inhibition. Specifically, the large orange-colored sodium decavanadate (V10O286-) anion was found to be a stronger inhibitor of growth of two mycobacterial species than the colorless oxovanadate prepared from sodium metavanadate. The vanadium(V) speciation in the growth media and conversion among species under growth conditions was monitored using 51V NMR spectroscopy and speciation calculations. The findings presented in this work is particularly important in considering the many applications of polyoxometalates in biological and medical studies, such as the investigation of the phase-problem in X-ray crystallography for the ribosome. The findings presented in this work investigate the interactions of oxometalates with other biological systems.https://www.frontiersin.org/article/10.3389/fchem.2018.00519/fullvanadatespeciationsodium metavanadatedecavanadategrowth inhibitiontuberculosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nuttaporn Samart Nuttaporn Samart Zeyad Arhouma Zeyad Arhouma Santosh Kumar Heide A. Murakami Dean C. Crick Dean C. Crick Debbie C. Crans Debbie C. Crans |
spellingShingle |
Nuttaporn Samart Nuttaporn Samart Zeyad Arhouma Zeyad Arhouma Santosh Kumar Heide A. Murakami Dean C. Crick Dean C. Crick Debbie C. Crans Debbie C. Crans Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates Frontiers in Chemistry vanadate speciation sodium metavanadate decavanadate growth inhibition tuberculosis |
author_facet |
Nuttaporn Samart Nuttaporn Samart Zeyad Arhouma Zeyad Arhouma Santosh Kumar Heide A. Murakami Dean C. Crick Dean C. Crick Debbie C. Crans Debbie C. Crans |
author_sort |
Nuttaporn Samart |
title |
Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates |
title_short |
Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates |
title_full |
Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates |
title_fullStr |
Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates |
title_full_unstemmed |
Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates |
title_sort |
decavanadate inhibits mycobacterial growth more potently than other oxovanadates |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Chemistry |
issn |
2296-2646 |
publishDate |
2018-11-01 |
description |
51V NMR spectroscopy is used to document, using speciation analysis, that one oxometalate is a more potent growth inhibitor of two Mycobacterial strains than other oxovanadates, thus demonstrating selectivity in its interaction with cells. Historically, oxometalates have had many applications in biological and medical studies, including study of the phase-problem in X-ray crystallography of the ribosome. The effect of different vanadate salts on the growth of Mycobacterium smegmatis (M. smeg) and Mycobacterium tuberculosis (M. tb) was investigated, and speciation was found to be critical for the observed growth inhibition. Specifically, the large orange-colored sodium decavanadate (V10O286-) anion was found to be a stronger inhibitor of growth of two mycobacterial species than the colorless oxovanadate prepared from sodium metavanadate. The vanadium(V) speciation in the growth media and conversion among species under growth conditions was monitored using 51V NMR spectroscopy and speciation calculations. The findings presented in this work is particularly important in considering the many applications of polyoxometalates in biological and medical studies, such as the investigation of the phase-problem in X-ray crystallography for the ribosome. The findings presented in this work investigate the interactions of oxometalates with other biological systems. |
topic |
vanadate speciation sodium metavanadate decavanadate growth inhibition tuberculosis |
url |
https://www.frontiersin.org/article/10.3389/fchem.2018.00519/full |
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