Investigation of Anti-Toxoplasma gondii Antibodies in Cats Using Sabin- Feldman Dye Test in Ankara in 2016
Objective:Toxoplasmosis, in which obligate intracellular protozoa Toxoplasma gondii (T.gondii) is the causative organism, is a multisystemic disease that can be seen all over the world and can impair all vertebrates. The only hosts known for T.gondii are members of Felidae family. Our study aimed to...
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Galenos Yayinevi
2019-03-01
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doaj-e60726dea0e64f5c945580493d422af62020-11-25T01:35:08ZengGalenos YayineviTürkiye Parazitoloji Dergisi2146-30772146-30772019-03-014315910.4274/tpd.galenos.2019.612613049054Investigation of Anti-Toxoplasma gondii Antibodies in Cats Using Sabin- Feldman Dye Test in Ankara in 2016Banuçiçek Yücesan0Cahit Babür1Nafiye Koç2Figen Sezen3Selçuk Kılıç4Yüksel Gürüz5 Halk Sağlığı Genel Müdürlüğü, Mikrobiyoloji Referans Laboratuvarları ve Biyolojik Ürünler Dairesi Başkanlığı Ulusal Parazitoloji Referans Laboratuvarı, Ankara, Türkiye Halk Sağlığı Genel Müdürlüğü, Mikrobiyoloji Referans Laboratuvarları ve Biyolojik Ürünler Dairesi Başkanlığı Ulusal Parazitoloji Referans Laboratuvarı, Ankara, Türkiye Ankara Üniversitesi Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Ankara, Türkiye Halk Sağlığı Genel Müdürlüğü, Sağlık Tehditleri, Erken Uyarı ve Cevap Dairesi Başkanlığı, Ankara , Türkiye Halk Sağlığı Genel Müdürlüğü, Mikrobiyoloji Referans Laboratuvarları ve Biyolojik Ürünler Dairesi Başkanlığı Ulusal Parazitoloji Referans Laboratuvarı, Ankara, Türkiye Ege Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, İzmir, Türkiye Objective:Toxoplasmosis, in which obligate intracellular protozoa Toxoplasma gondii (T.gondii) is the causative organism, is a multisystemic disease that can be seen all over the world and can impair all vertebrates. The only hosts known for T.gondii are members of Felidae family. Our study aimed to determine anti-Toxoplasma gondii antibodies with Sabin-Feldman Dye Test (SFDT) in cats in Ankara. It’s aimed to evaluate the current situation in terms of Toxoplasmosis spread by comparing our findings with previous studies in the same region.Methods:Rh strain of Toxoplasma used in our study is maintained in our laboratory. SFDT is still accepted as the gold standard. Material of the study was obtained by taking blood samples from cats who were admitted to the clinics between March 2016 and October 2016 in Ankara. Blood samples were inactivated and measurements were done with SFDT 1/4, 1/16, 1/64, 1/256, 1/1024 titers.Results:SFDT resulted positive in 56 (43.4%) cats at a dilution of 1/16, in 7 (5.4%) cats at a dilution of 1/64, in 23 (17.8%) cats at a dilution of 1/256 and negative in 43 (33.3%) cats. Comparison of demographic data with SFDT results showed that positive test results did not differ according to gender and age (P=0.803 and P=0.991, respectively). Seropositivity was higher in stray cats than house cats (P<0.001). Test results were negative in the cats that fed only by commercial dry food (P<0.001). Positivity in hunter cats was more than in non-hunters (P<0.001).Conclusion:Seropositivity was detected in 66.6% of the cats, which was quite a high rate. As a result, taking precautions in terms of Toxoplasma for stray cats that are hunting and feeding naturally is a necessity for public health. http://www.turkiyeparazitolderg.org/archives/archive-detail/article-preview/nvestigation-of-anti-itoxoplasma-gondii-i-antibodi/26287 Toxoplasma gondiiSabin-feldman dye testcats |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Banuçiçek Yücesan Cahit Babür Nafiye Koç Figen Sezen Selçuk Kılıç Yüksel Gürüz |
spellingShingle |
Banuçiçek Yücesan Cahit Babür Nafiye Koç Figen Sezen Selçuk Kılıç Yüksel Gürüz Investigation of Anti-Toxoplasma gondii Antibodies in Cats Using Sabin- Feldman Dye Test in Ankara in 2016 Türkiye Parazitoloji Dergisi Toxoplasma gondii Sabin-feldman dye test cats |
author_facet |
Banuçiçek Yücesan Cahit Babür Nafiye Koç Figen Sezen Selçuk Kılıç Yüksel Gürüz |
author_sort |
Banuçiçek Yücesan |
title |
Investigation of Anti-Toxoplasma gondii Antibodies in Cats Using Sabin- Feldman Dye Test in Ankara in 2016 |
title_short |
Investigation of Anti-Toxoplasma gondii Antibodies in Cats Using Sabin- Feldman Dye Test in Ankara in 2016 |
title_full |
Investigation of Anti-Toxoplasma gondii Antibodies in Cats Using Sabin- Feldman Dye Test in Ankara in 2016 |
title_fullStr |
Investigation of Anti-Toxoplasma gondii Antibodies in Cats Using Sabin- Feldman Dye Test in Ankara in 2016 |
title_full_unstemmed |
Investigation of Anti-Toxoplasma gondii Antibodies in Cats Using Sabin- Feldman Dye Test in Ankara in 2016 |
title_sort |
investigation of anti-toxoplasma gondii antibodies in cats using sabin- feldman dye test in ankara in 2016 |
publisher |
Galenos Yayinevi |
series |
Türkiye Parazitoloji Dergisi |
issn |
2146-3077 2146-3077 |
publishDate |
2019-03-01 |
description |
Objective:Toxoplasmosis, in which obligate intracellular protozoa Toxoplasma gondii (T.gondii) is the causative organism, is a multisystemic disease that can be seen all over the world and can impair all vertebrates. The only hosts known for T.gondii are members of Felidae family. Our study aimed to determine anti-Toxoplasma gondii antibodies with Sabin-Feldman Dye Test (SFDT) in cats in Ankara. It’s aimed to evaluate the current situation in terms of Toxoplasmosis spread by comparing our findings with previous studies in the same region.Methods:Rh strain of Toxoplasma used in our study is maintained in our laboratory. SFDT is still accepted as the gold standard. Material of the study was obtained by taking blood samples from cats who were admitted to the clinics between March 2016 and October 2016 in Ankara. Blood samples were inactivated and measurements were done with SFDT 1/4, 1/16, 1/64, 1/256, 1/1024 titers.Results:SFDT resulted positive in 56 (43.4%) cats at a dilution of 1/16, in 7 (5.4%) cats at a dilution of 1/64, in 23 (17.8%) cats at a dilution of 1/256 and negative in 43 (33.3%) cats. Comparison of demographic data with SFDT results showed that positive test results did not differ according to gender and age (P=0.803 and P=0.991, respectively). Seropositivity was higher in stray cats than house cats (P<0.001). Test results were negative in the cats that fed only by commercial dry food (P<0.001). Positivity in hunter cats was more than in non-hunters (P<0.001).Conclusion:Seropositivity was detected in 66.6% of the cats, which was quite a high rate. As a result, taking precautions in terms of Toxoplasma for stray cats that are hunting and feeding naturally is a necessity for public health. |
topic |
Toxoplasma gondii Sabin-feldman dye test cats |
url |
http://www.turkiyeparazitolderg.org/archives/archive-detail/article-preview/nvestigation-of-anti-itoxoplasma-gondii-i-antibodi/26287
|
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