A 2cM genome-wide scan of European Holstein cattle affected by classical BSE
<p>Abstract</p> <p>Background</p> <p>Classical bovine spongiform encephalopathy (BSE) is an acquired prion disease that is invariably fatal in cattle and has been implicated as a significant human health risk. Polymorphisms that alter the prion protein of sheep or human...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2010-03-01
|
Series: | BMC Genetics |
Online Access: | http://www.biomedcentral.com/1471-2156/11/20 |
id |
doaj-e621be2513c34edca8324dc43bb872e2 |
---|---|
record_format |
Article |
spelling |
doaj-e621be2513c34edca8324dc43bb872e22020-11-25T03:22:10ZengBMCBMC Genetics1471-21562010-03-011112010.1186/1471-2156-11-20A 2cM genome-wide scan of European Holstein cattle affected by classical BSEPrasad AparnaMcKay StephanieSettles MatthewStothard PaulLaegreid William WClawson Michael LMurdoch Brenda MWang ZhiquanMoore Stephen SWilliams John L<p>Abstract</p> <p>Background</p> <p>Classical bovine spongiform encephalopathy (BSE) is an acquired prion disease that is invariably fatal in cattle and has been implicated as a significant human health risk. Polymorphisms that alter the prion protein of sheep or humans have been associated with variations in transmissible spongiform encephalopathy susceptibility or resistance. In contrast, there is no strong evidence that non-synonymous mutations in the bovine prion gene (<it>PRNP</it>) are associated with classical BSE disease susceptibility. However, two bovine <it>PRNP </it>insertion/deletion polymorphisms, one within the promoter region and the other in intron 1, have been associated with susceptibility to classical BSE. These associations do not explain the full extent of BSE susceptibility, and loci outside of <it>PRNP </it>appear to be associated with disease incidence in some cattle populations. To test for associations with BSE susceptibility, we conducted a genome wide scan using a panel of 3,072 single nucleotide polymorphism (SNP) markers on 814 animals representing cases and control Holstein cattle from the United Kingdom BSE epidemic.</p> <p>Results</p> <p>Two sets of BSE affected Holstein cattle were analyzed in this study, one set with known family relationships and the second set of paired cases with controls. The family set comprises half-sibling progeny from six sires. The progeny from four of these sires had previously been scanned with microsatellite markers. The results obtained from the current analysis of the family set yielded both some supporting and new results compared with those obtained in the earlier study. The results revealed 27 SNPs representing 18 chromosomes associated with incidence of BSE disease. These results confirm a region previously reported on chromosome 20, and identify additional regions on chromosomes 2, 14, 16, 21 and 28. This study did not identify a significant association near the <it>PRNP </it>in the family sample set. The only association found in the <it>PRNP </it>region was in the case-control sample set and this was not significant after multiple test correction. The genome scan of the case-control animals did not identify any associations that passed a stringent genome-wide significance threshold.</p> <p>Conclusions</p> <p>Several regions of the genome are statistically associated with the incidence of classical BSE in European Holstein cattle. Further investigation of loci on chromosomes 2, 14, 16, 20, 21 and 28 will be required to uncover any biological significance underlying these marker associations.</p> http://www.biomedcentral.com/1471-2156/11/20 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Prasad Aparna McKay Stephanie Settles Matthew Stothard Paul Laegreid William W Clawson Michael L Murdoch Brenda M Wang Zhiquan Moore Stephen S Williams John L |
spellingShingle |
Prasad Aparna McKay Stephanie Settles Matthew Stothard Paul Laegreid William W Clawson Michael L Murdoch Brenda M Wang Zhiquan Moore Stephen S Williams John L A 2cM genome-wide scan of European Holstein cattle affected by classical BSE BMC Genetics |
author_facet |
Prasad Aparna McKay Stephanie Settles Matthew Stothard Paul Laegreid William W Clawson Michael L Murdoch Brenda M Wang Zhiquan Moore Stephen S Williams John L |
author_sort |
Prasad Aparna |
title |
A 2cM genome-wide scan of European Holstein cattle affected by classical BSE |
title_short |
A 2cM genome-wide scan of European Holstein cattle affected by classical BSE |
title_full |
A 2cM genome-wide scan of European Holstein cattle affected by classical BSE |
title_fullStr |
A 2cM genome-wide scan of European Holstein cattle affected by classical BSE |
title_full_unstemmed |
A 2cM genome-wide scan of European Holstein cattle affected by classical BSE |
title_sort |
2cm genome-wide scan of european holstein cattle affected by classical bse |
publisher |
BMC |
series |
BMC Genetics |
issn |
1471-2156 |
publishDate |
2010-03-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Classical bovine spongiform encephalopathy (BSE) is an acquired prion disease that is invariably fatal in cattle and has been implicated as a significant human health risk. Polymorphisms that alter the prion protein of sheep or humans have been associated with variations in transmissible spongiform encephalopathy susceptibility or resistance. In contrast, there is no strong evidence that non-synonymous mutations in the bovine prion gene (<it>PRNP</it>) are associated with classical BSE disease susceptibility. However, two bovine <it>PRNP </it>insertion/deletion polymorphisms, one within the promoter region and the other in intron 1, have been associated with susceptibility to classical BSE. These associations do not explain the full extent of BSE susceptibility, and loci outside of <it>PRNP </it>appear to be associated with disease incidence in some cattle populations. To test for associations with BSE susceptibility, we conducted a genome wide scan using a panel of 3,072 single nucleotide polymorphism (SNP) markers on 814 animals representing cases and control Holstein cattle from the United Kingdom BSE epidemic.</p> <p>Results</p> <p>Two sets of BSE affected Holstein cattle were analyzed in this study, one set with known family relationships and the second set of paired cases with controls. The family set comprises half-sibling progeny from six sires. The progeny from four of these sires had previously been scanned with microsatellite markers. The results obtained from the current analysis of the family set yielded both some supporting and new results compared with those obtained in the earlier study. The results revealed 27 SNPs representing 18 chromosomes associated with incidence of BSE disease. These results confirm a region previously reported on chromosome 20, and identify additional regions on chromosomes 2, 14, 16, 21 and 28. This study did not identify a significant association near the <it>PRNP </it>in the family sample set. The only association found in the <it>PRNP </it>region was in the case-control sample set and this was not significant after multiple test correction. The genome scan of the case-control animals did not identify any associations that passed a stringent genome-wide significance threshold.</p> <p>Conclusions</p> <p>Several regions of the genome are statistically associated with the incidence of classical BSE in European Holstein cattle. Further investigation of loci on chromosomes 2, 14, 16, 20, 21 and 28 will be required to uncover any biological significance underlying these marker associations.</p> |
url |
http://www.biomedcentral.com/1471-2156/11/20 |
work_keys_str_mv |
AT prasadaparna a2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT mckaystephanie a2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT settlesmatthew a2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT stothardpaul a2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT laegreidwilliamw a2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT clawsonmichaell a2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT murdochbrendam a2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT wangzhiquan a2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT moorestephens a2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT williamsjohnl a2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT prasadaparna 2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT mckaystephanie 2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT settlesmatthew 2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT stothardpaul 2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT laegreidwilliamw 2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT clawsonmichaell 2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT murdochbrendam 2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT wangzhiquan 2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT moorestephens 2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse AT williamsjohnl 2cmgenomewidescanofeuropeanholsteincattleaffectedbyclassicalbse |
_version_ |
1724610764605489152 |