Effects of Bioprocess Parameters on Production of Cellulase using Miscanthus as Substrate

• Main Authors: Adrian Trulea, Teodor Vintilă, Georgeta Pop, Monica Dragomirescu
• Format: Article
• Language: English
• Published: Agroprint Timisoara 2013-05-01
• Series: Scientific Papers Animal Science and Biotechnologies
• Subjects: cellulase; fermentation; fungi; miscanthus
• Online Access: http://spasb.ro/index.php/spasb/article/view/107
• ISSN: 1841-9364; 2344-4576

A number of fungi were isolated abroad and screened for cellulolytic potential. In this work we studied cellulase production with several fungi strains, using Miscanthus giganteus as substrate. An indigenous strain of T. viride was selected and the kinetics of cellulase production is reported. Product formation parameters of different types of cellulases indicate that the studied strain of T. viride is capable of producing important levels of cellulases when grown on Mandels medium with Miscanthus biomass as carbon source. Furthermore, it was observed that production of endoglucanase reaches its maximum during exponential phase of growth, while exoglucanase during the stationary phase. Enzyme production by solid-state fermentation was also investigated and found to be more efficient than liquid state fermentation. High production of cellulase was noted at the following parameters for liquid cultures: 4% Miscanthus giganteus biomass, 5% inoculum, 180 r.p.m. agitation, pH 5; and 60% humidity in the case of solid state fermentation. Comparing the productions of cellulases in the fermentations carried out in this study, data indicate Miscanthus as ideal substrate for cellulase biosynthesis with fungi.