Effect of ErhBMP-2-loaded β-tricalcium phosphate on ulna defects in the osteoporosis rabbit model
Purpose: Autografts, the gold standard treatment for large bone defects, present complications, especially in conditions with reduced bone-repair capacity, such as osteoporosis. Escherichia coli-derived recombinant human bone morphogenesis protein-2 (ErhBMP-2), was used in this study to improve the...
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doaj-e6352715851b44d1bfcda8ef65f6c6cf2021-06-03T04:57:17ZengElsevierBone Reports2352-18722021-06-0114100739Effect of ErhBMP-2-loaded β-tricalcium phosphate on ulna defects in the osteoporosis rabbit modelTse-Yin Huang0Chang-Chin Wu1Pei-Wei Weng2Jian-Ming Chen3Wen-Ling Yeh4Ph.D. Program for Biotech Pharmaceutical Industry, School of Pharmacy, China Medical University, Taichung 40402, TaiwanDepartment of Orthopedics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei 10002, Taiwan; Department of Biomedical Engineering, Yuanpei University of Medical Technology, Hsinchu 30015, Taiwan; Department of Orthopedics, En Chu Kong Hospital, New Taipei City 23702, TaiwanDepartment of Orthopaedics, School of Medicine, College of Medicine, Taipei Medical University, Taiwan; Department of Orthopaedics, Shuang Ho Hospital, Taipei Medical University, TaiwanDepartment of Orthopedic Surgery, Chang Gung Memorial Hospital-Linkou, Tao-Yuan 33305, Taiwan; Corresponding author at: Department of Orthopedic Surgery, Chang Gung Memorial Hospital-Linkou, Tao-Yuan 33305, Taiwan.Department of Orthopedic Surgery, Chang Gung Memorial Hospital-Linkou, Tao-Yuan 33305, Taiwan; Bone and Joint Research Center, Chang Gung Memorial Hospital, Taoyuan, TaiwanPurpose: Autografts, the gold standard treatment for large bone defects, present complications, especially in conditions with reduced bone-repair capacity, such as osteoporosis. Escherichia coli-derived recombinant human bone morphogenesis protein-2 (ErhBMP-2), was used in this study to improve the osteoinductivity of β-tricalcium phosphate (β-TCP). This study evaluated the bone-repair capacity of ErhBMP-2-loaded β-TCP on osteoporosis rabbit model, relative to the sole use of autograft and β-TCP treatments. Methods: The osteoporosis rabbit model was induced through ovariectomy and glucocorticoid dosing; 2-cm segmental ulnar defects were created, which were treated with either autograft, β-TCP alone, or ErhBMP-2-loaded β-TCP or left untreated. The quality of newly formed ulnae was evaluated 8 weeks after ulnar surgery through micro-CT, biomechanical, histological, and histomorphometric assessments. Results: The osteoporosis rabbit model was developed and maintained till the end of the study. The maximal load and stiffness in the ErhBMP-2-loaded TCP group were significantly higher than those in the autograft group, whereas the TCP-alone group performed similarly as did the untreated group in the force loading and stiffness tests. According to the micro-CT evaluation, the ErhBMP-2-loaded TCP group had significantly higher bone volume relative to the autograft and TCP-alone groups. Histological assessments revealed better defect bridging and marrow formation in the ErhBMP-2-loaded TCP group relative to the TCP-alone group. Mineral apposition rates were significantly higher in the ErhBMP-2-loaded TCP and autograft groups than in the TCP-alone and untreated groups. Conclusion: Relative to autografts, ErhBMP-2-loaded TCP, as an alternative grafting material, provides better or comparable healing on critical-sized long bone defects in the osteoporosis rabbit model.http://www.sciencedirect.com/science/article/pii/S235218722030499XUlna defectOsteoporosis rabbit modelErhBMP-2Tricalcium phosphate |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tse-Yin Huang Chang-Chin Wu Pei-Wei Weng Jian-Ming Chen Wen-Ling Yeh |
spellingShingle |
Tse-Yin Huang Chang-Chin Wu Pei-Wei Weng Jian-Ming Chen Wen-Ling Yeh Effect of ErhBMP-2-loaded β-tricalcium phosphate on ulna defects in the osteoporosis rabbit model Bone Reports Ulna defect Osteoporosis rabbit model ErhBMP-2 Tricalcium phosphate |
author_facet |
Tse-Yin Huang Chang-Chin Wu Pei-Wei Weng Jian-Ming Chen Wen-Ling Yeh |
author_sort |
Tse-Yin Huang |
title |
Effect of ErhBMP-2-loaded β-tricalcium phosphate on ulna defects in the osteoporosis rabbit model |
title_short |
Effect of ErhBMP-2-loaded β-tricalcium phosphate on ulna defects in the osteoporosis rabbit model |
title_full |
Effect of ErhBMP-2-loaded β-tricalcium phosphate on ulna defects in the osteoporosis rabbit model |
title_fullStr |
Effect of ErhBMP-2-loaded β-tricalcium phosphate on ulna defects in the osteoporosis rabbit model |
title_full_unstemmed |
Effect of ErhBMP-2-loaded β-tricalcium phosphate on ulna defects in the osteoporosis rabbit model |
title_sort |
effect of erhbmp-2-loaded β-tricalcium phosphate on ulna defects in the osteoporosis rabbit model |
publisher |
Elsevier |
series |
Bone Reports |
issn |
2352-1872 |
publishDate |
2021-06-01 |
description |
Purpose: Autografts, the gold standard treatment for large bone defects, present complications, especially in conditions with reduced bone-repair capacity, such as osteoporosis. Escherichia coli-derived recombinant human bone morphogenesis protein-2 (ErhBMP-2), was used in this study to improve the osteoinductivity of β-tricalcium phosphate (β-TCP). This study evaluated the bone-repair capacity of ErhBMP-2-loaded β-TCP on osteoporosis rabbit model, relative to the sole use of autograft and β-TCP treatments. Methods: The osteoporosis rabbit model was induced through ovariectomy and glucocorticoid dosing; 2-cm segmental ulnar defects were created, which were treated with either autograft, β-TCP alone, or ErhBMP-2-loaded β-TCP or left untreated. The quality of newly formed ulnae was evaluated 8 weeks after ulnar surgery through micro-CT, biomechanical, histological, and histomorphometric assessments. Results: The osteoporosis rabbit model was developed and maintained till the end of the study. The maximal load and stiffness in the ErhBMP-2-loaded TCP group were significantly higher than those in the autograft group, whereas the TCP-alone group performed similarly as did the untreated group in the force loading and stiffness tests. According to the micro-CT evaluation, the ErhBMP-2-loaded TCP group had significantly higher bone volume relative to the autograft and TCP-alone groups. Histological assessments revealed better defect bridging and marrow formation in the ErhBMP-2-loaded TCP group relative to the TCP-alone group. Mineral apposition rates were significantly higher in the ErhBMP-2-loaded TCP and autograft groups than in the TCP-alone and untreated groups. Conclusion: Relative to autografts, ErhBMP-2-loaded TCP, as an alternative grafting material, provides better or comparable healing on critical-sized long bone defects in the osteoporosis rabbit model. |
topic |
Ulna defect Osteoporosis rabbit model ErhBMP-2 Tricalcium phosphate |
url |
http://www.sciencedirect.com/science/article/pii/S235218722030499X |
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