Effect of the nanoliposomal formulations of rifampin and N-acetyl cysteine on staphylococcus epidermidis biofilm
Objective(s): Staphylococcus epidermidis is a common cause of medical device-associated infections due to biofilm formation, and its elimination is extremely challenging. Although rifampin efficacy against S. epidermidis biofilms has been confirmed, its use as a single agent may lead to resistance....
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Mashhad University of Medical Sciences
2020-04-01
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doaj-e6416bf2ebf84fdba1aea0670cf4f3662020-11-25T02:01:04ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042020-04-017213113710.22038/nmj.2020.07.00614574Effect of the nanoliposomal formulations of rifampin and N-acetyl cysteine on staphylococcus epidermidis biofilmFarzaneh Bazrgari0Bahman Khameneh1Bibi Sedigheh Fazly Bazzaz2Asma Mahmoudi3Bizhan Malaekeh-Nikouei4School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranSchool of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranBiotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranSchool of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranNanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranObjective(s): Staphylococcus epidermidis is a common cause of medical device-associated infections due to biofilm formation, and its elimination is extremely challenging. Although rifampin efficacy against S. epidermidis biofilms has been confirmed, its use as a single agent may lead to resistance. As such, it is assumed that the combination of rifampin and N-acetylcysteine (NAC) could exert additive effects as a mucolytic agent. The present study aimed to use a liposomal system for the delivery of these compounds to bacterial biofilm.<br />Materials and Methods: Liposomal formulations were prepared using the dehydration-rehydration method and characterized in terms of the size, zeta potential, and encapsulation efficacy. In addition, the ability of various formulations in the eradication of bacterial biofilm and inhibition of biofilm formation was assessed based on the optical density ratio. <br />Results: The zeta potential of the liposomes was positive, and the mean size of these liposomal formulations was less than 200 nanometers. Liposomal rifampin was the most effective formulation against S. epidermidis, and the anti-biofilm activity of most of the formulations was concentration-dependent and time-dependent.<br />Conclusion: According to the results, the rifampin-loaded liposomes were effective against S. epidermidis biofilm formation.http://nmj.mums.ac.ir/article_14574_e572ab9f30c5001ff9612c72c6252f44.pdfbiofilmnanoliposomesn-acetyl cysteinerifampin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Farzaneh Bazrgari Bahman Khameneh Bibi Sedigheh Fazly Bazzaz Asma Mahmoudi Bizhan Malaekeh-Nikouei |
spellingShingle |
Farzaneh Bazrgari Bahman Khameneh Bibi Sedigheh Fazly Bazzaz Asma Mahmoudi Bizhan Malaekeh-Nikouei Effect of the nanoliposomal formulations of rifampin and N-acetyl cysteine on staphylococcus epidermidis biofilm Nanomedicine Journal biofilm nanoliposomes n-acetyl cysteine rifampin |
author_facet |
Farzaneh Bazrgari Bahman Khameneh Bibi Sedigheh Fazly Bazzaz Asma Mahmoudi Bizhan Malaekeh-Nikouei |
author_sort |
Farzaneh Bazrgari |
title |
Effect of the nanoliposomal formulations of rifampin and N-acetyl cysteine on staphylococcus epidermidis biofilm |
title_short |
Effect of the nanoliposomal formulations of rifampin and N-acetyl cysteine on staphylococcus epidermidis biofilm |
title_full |
Effect of the nanoliposomal formulations of rifampin and N-acetyl cysteine on staphylococcus epidermidis biofilm |
title_fullStr |
Effect of the nanoliposomal formulations of rifampin and N-acetyl cysteine on staphylococcus epidermidis biofilm |
title_full_unstemmed |
Effect of the nanoliposomal formulations of rifampin and N-acetyl cysteine on staphylococcus epidermidis biofilm |
title_sort |
effect of the nanoliposomal formulations of rifampin and n-acetyl cysteine on staphylococcus epidermidis biofilm |
publisher |
Mashhad University of Medical Sciences |
series |
Nanomedicine Journal |
issn |
2322-3049 2322-5904 |
publishDate |
2020-04-01 |
description |
Objective(s): Staphylococcus epidermidis is a common cause of medical device-associated infections due to biofilm formation, and its elimination is extremely challenging. Although rifampin efficacy against S. epidermidis biofilms has been confirmed, its use as a single agent may lead to resistance. As such, it is assumed that the combination of rifampin and N-acetylcysteine (NAC) could exert additive effects as a mucolytic agent. The present study aimed to use a liposomal system for the delivery of these compounds to bacterial biofilm.<br />Materials and Methods: Liposomal formulations were prepared using the dehydration-rehydration method and characterized in terms of the size, zeta potential, and encapsulation efficacy. In addition, the ability of various formulations in the eradication of bacterial biofilm and inhibition of biofilm formation was assessed based on the optical density ratio. <br />Results: The zeta potential of the liposomes was positive, and the mean size of these liposomal formulations was less than 200 nanometers. Liposomal rifampin was the most effective formulation against S. epidermidis, and the anti-biofilm activity of most of the formulations was concentration-dependent and time-dependent.<br />Conclusion: According to the results, the rifampin-loaded liposomes were effective against S. epidermidis biofilm formation. |
topic |
biofilm nanoliposomes n-acetyl cysteine rifampin |
url |
http://nmj.mums.ac.ir/article_14574_e572ab9f30c5001ff9612c72c6252f44.pdf |
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