The transcriptional reprograming and functional identification of WRKY family members in pepper’s response to Phytophthora capsici infection
Abstract Background Plant transcription factors (TFs) are key transcriptional regulators to manipulate the regulatory network of host immunity. However, the globally transcriptional reprogramming of plant TF families in response to pathogens, especially between the resistant and susceptible host pla...
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doaj-e65279c27ad3447a87760dd415ad5f802020-11-25T02:48:58ZengBMCBMC Plant Biology1471-22292020-06-0120111410.1186/s12870-020-02464-7The transcriptional reprograming and functional identification of WRKY family members in pepper’s response to Phytophthora capsici infectionWei Cheng0Yan Jiang1Jiangtao Peng2Jianwen Guo3Menglan Lin4Chengting Jin5Jinfeng Huang6Weiqi Tang7Deyi Guan8Shuilin He9National Education Minister Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry UniversityNational Education Minister Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry UniversityCollege of Agriculture, Fujian Agriculture and Forestry UniversityNational Education Minister Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry UniversityNational Education Minister Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry UniversityCollege of Agriculture, Fujian Agriculture and Forestry UniversityNational Education Minister Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry UniversityCollege of Agriculture, Fujian Agriculture and Forestry UniversityNational Education Minister Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry UniversityNational Education Minister Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry UniversityAbstract Background Plant transcription factors (TFs) are key transcriptional regulators to manipulate the regulatory network of host immunity. However, the globally transcriptional reprogramming of plant TF families in response to pathogens, especially between the resistant and susceptible host plants, remains largely unknown. Results Here, we performed time-series RNA-seq from a resistant pepper line CM334 and a susceptible pepper line EC01 upon challenged with Phytophthora capsici, and enrichment analysis indicated that WRKY family most significantly enriched in both CM334 and EC01. Interestingly, we found that nearly half of the WRKY family members were significantly up-regulated, whereas none of them were down-regulated in the two lines. These induced WRKY genes were greatly overlapped between CM334 and EC01. More strikingly, most of these induced WRKY genes were expressed in time-order patterns, and could be mainly divided into three subgroups: early response (3 h-up), mid response (24 h-up) and mid-late response (ML-up) genes. Moreover, it was found that the responses of these ML-up genes were several hours delayed in EC01. Furthermore, a total of 19 induced WRKY genes were selected for functional identification by virus-induced gene silencing. The result revealed that silencing of CaWRKY03–6, CaWRKY03–7, CaWRKY06–5 or CaWRKY10–4 significantly increase the susceptibility to P. capsici both in CM334 and EC01, indicating that they might contribute to pepper’s basal defense against P. capsici; while silencing of CaWRKY08–4 and CaWRKY01–10 significantly impaired the disease resistance in CM334 but not in EC01, suggesting that these two WRKY genes are prominent modulators specifically in the resistant pepper plants. Conclusions These results considerably extend our understanding of WRKY gene family in pepper’s resistance against P. capsici and provide potential applications for genetic improvement against phytophthora blight.http://link.springer.com/article/10.1186/s12870-020-02464-7PepperPhytophthoraWRKYTranscription factorTranscriptomeTranscriptional reprograming |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei Cheng Yan Jiang Jiangtao Peng Jianwen Guo Menglan Lin Chengting Jin Jinfeng Huang Weiqi Tang Deyi Guan Shuilin He |
spellingShingle |
Wei Cheng Yan Jiang Jiangtao Peng Jianwen Guo Menglan Lin Chengting Jin Jinfeng Huang Weiqi Tang Deyi Guan Shuilin He The transcriptional reprograming and functional identification of WRKY family members in pepper’s response to Phytophthora capsici infection BMC Plant Biology Pepper Phytophthora WRKY Transcription factor Transcriptome Transcriptional reprograming |
author_facet |
Wei Cheng Yan Jiang Jiangtao Peng Jianwen Guo Menglan Lin Chengting Jin Jinfeng Huang Weiqi Tang Deyi Guan Shuilin He |
author_sort |
Wei Cheng |
title |
The transcriptional reprograming and functional identification of WRKY family members in pepper’s response to Phytophthora capsici infection |
title_short |
The transcriptional reprograming and functional identification of WRKY family members in pepper’s response to Phytophthora capsici infection |
title_full |
The transcriptional reprograming and functional identification of WRKY family members in pepper’s response to Phytophthora capsici infection |
title_fullStr |
The transcriptional reprograming and functional identification of WRKY family members in pepper’s response to Phytophthora capsici infection |
title_full_unstemmed |
The transcriptional reprograming and functional identification of WRKY family members in pepper’s response to Phytophthora capsici infection |
title_sort |
transcriptional reprograming and functional identification of wrky family members in pepper’s response to phytophthora capsici infection |
publisher |
BMC |
series |
BMC Plant Biology |
issn |
1471-2229 |
publishDate |
2020-06-01 |
description |
Abstract Background Plant transcription factors (TFs) are key transcriptional regulators to manipulate the regulatory network of host immunity. However, the globally transcriptional reprogramming of plant TF families in response to pathogens, especially between the resistant and susceptible host plants, remains largely unknown. Results Here, we performed time-series RNA-seq from a resistant pepper line CM334 and a susceptible pepper line EC01 upon challenged with Phytophthora capsici, and enrichment analysis indicated that WRKY family most significantly enriched in both CM334 and EC01. Interestingly, we found that nearly half of the WRKY family members were significantly up-regulated, whereas none of them were down-regulated in the two lines. These induced WRKY genes were greatly overlapped between CM334 and EC01. More strikingly, most of these induced WRKY genes were expressed in time-order patterns, and could be mainly divided into three subgroups: early response (3 h-up), mid response (24 h-up) and mid-late response (ML-up) genes. Moreover, it was found that the responses of these ML-up genes were several hours delayed in EC01. Furthermore, a total of 19 induced WRKY genes were selected for functional identification by virus-induced gene silencing. The result revealed that silencing of CaWRKY03–6, CaWRKY03–7, CaWRKY06–5 or CaWRKY10–4 significantly increase the susceptibility to P. capsici both in CM334 and EC01, indicating that they might contribute to pepper’s basal defense against P. capsici; while silencing of CaWRKY08–4 and CaWRKY01–10 significantly impaired the disease resistance in CM334 but not in EC01, suggesting that these two WRKY genes are prominent modulators specifically in the resistant pepper plants. Conclusions These results considerably extend our understanding of WRKY gene family in pepper’s resistance against P. capsici and provide potential applications for genetic improvement against phytophthora blight. |
topic |
Pepper Phytophthora WRKY Transcription factor Transcriptome Transcriptional reprograming |
url |
http://link.springer.com/article/10.1186/s12870-020-02464-7 |
work_keys_str_mv |
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