Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification
Apple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reve...
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doaj-e657c9bfd5fd4d669bff27c984af10a22020-11-24T23:48:13ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542018-12-0134657557910.5423/PPJ.NT.06.2018.010810.5423PPJ.NT.06.2018.0108Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase AmplificationNam-Yeon Kim0Jonghee Oh1Su-Heon Lee2Hongsup Kim3Jae Sun Moon4Rae-Dong Jeong5Department of Applied Biology, Institute of Environmentally Friendly Agriculture, Chonnam National University, Gwangju 61185, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 98411, KoreaSchool of Applied Biosciences, Kyungpook National University, Daegu 98411, KoreaSeed Testing & Research Center, Korea Seed & Variety Service, Gimcheon, KoreaPlant Genome Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon, KoreaDepartment of Applied Biology, Institute of Environmentally Friendly Agriculture, Chonnam National University, Gwangju 61185, KoreaApple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA obtained from an ASGV-infected plant. The major advantage of this assay is that the reaction for the target virus is completed in 1 min, and amplification only requires an incubation temperature of 42°C. This assay is a promising alternative method for pear breeding programs or virus-free certification laboratories.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305176Apple stem grooving virusmolecular diagnosisreverse transcription-recombinase polymerase amplification |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nam-Yeon Kim Jonghee Oh Su-Heon Lee Hongsup Kim Jae Sun Moon Rae-Dong Jeong |
spellingShingle |
Nam-Yeon Kim Jonghee Oh Su-Heon Lee Hongsup Kim Jae Sun Moon Rae-Dong Jeong Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification The Plant Pathology Journal Apple stem grooving virus molecular diagnosis reverse transcription-recombinase polymerase amplification |
author_facet |
Nam-Yeon Kim Jonghee Oh Su-Heon Lee Hongsup Kim Jae Sun Moon Rae-Dong Jeong |
author_sort |
Nam-Yeon Kim |
title |
Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification |
title_short |
Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification |
title_full |
Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification |
title_fullStr |
Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification |
title_full_unstemmed |
Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification |
title_sort |
rapid and specific detection of apple stem grooving virus by reverse transcription-recombinase polymerase amplification |
publisher |
Hanrimwon Publishing Company |
series |
The Plant Pathology Journal |
issn |
1598-2254 |
publishDate |
2018-12-01 |
description |
Apple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA obtained from an ASGV-infected plant. The major advantage of this assay is that the reaction for the target virus is completed in 1 min, and amplification only requires an incubation temperature of 42°C. This assay is a promising alternative method for pear breeding programs or virus-free certification laboratories. |
topic |
Apple stem grooving virus molecular diagnosis reverse transcription-recombinase polymerase amplification |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305176 |
work_keys_str_mv |
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