Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification

• Main Authors: Nam-Yeon Kim, Jonghee Oh, Su-Heon Lee, Hongsup Kim, Jae Sun Moon, Rae-Dong Jeong
• Format: Article
• Language: English
• Published: Hanrimwon Publishing Company 2018-12-01
• Series: The Plant Pathology Journal
• Subjects: Apple stem grooving virus; molecular diagnosis; reverse transcription-recombinase polymerase amplification
• Online Access: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305176

Apple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA obtained from an ASGV-infected plant. The major advantage of this assay is that the reaction for the target virus is completed in 1 min, and amplification only requires an incubation temperature of 42°C. This assay is a promising alternative method for pear breeding programs or virus-free certification laboratories.