Identification of differentially expressed genes and signaling pathways in human conjunctiva and reproductive tract infected with Chlamydia trachomatis
Abstract Background Currently, Chlamydia trachomatis–specific host defense mechanisms in humans remain poorly defined. To study the characteristics of host cells infected early with Chlamydia trachomatis, we used bioinformatics methods to analyze the RNA transcription profiles of the conjunctiva, fa...
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doaj-e6649ccb5b37489d97940a5fabb422702021-04-25T11:49:15ZengBMCHuman Genomics1479-73642021-04-0115111510.1186/s40246-021-00313-8Identification of differentially expressed genes and signaling pathways in human conjunctiva and reproductive tract infected with Chlamydia trachomatisGuo-Dong Zhu0Xun-Jie Cao1Ya-Ping Li2Jia-Xin Li3Zi-Jian Leng4Li-Min Xie5Xu-Guang Guo6Departments of Geriatrics and Oncology, Guangzhou First People’s Hospital, School of Medicine, South China University of TechnologyDepartment of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical UniversityDepartment of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical UniversityDepartment of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical UniversityDepartment of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical UniversityDepartment of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical UniversityDepartment of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical UniversityAbstract Background Currently, Chlamydia trachomatis–specific host defense mechanisms in humans remain poorly defined. To study the characteristics of host cells infected early with Chlamydia trachomatis, we used bioinformatics methods to analyze the RNA transcription profiles of the conjunctiva, fallopian tubes, and endometrium in humans infected with Chlamydia trachomatis. Method The gene expression profiles of GSE20430, GSE20436, GSE26692, and GSE41075 were downloaded from the Gene Expression Synthesis (GEO) database. Then, we obtained the differentially expressed genes (DEGs) through the R 4.0.1 software. STRING was used to construct protein–protein interaction (PPI) networks; then, the Cytoscape 3.7.2 software was used to visualize the PPI and screen hub genes. GraphPad Prism 8.0 software was used to verify the expression of the hub gene. In addition, the gene–miRNA interaction was constructed on the NetworkAnalyst 3.0 platform using the miRTarBase v8.0 database. Results A total of 600 and 135 DEGs were screened out in the conjunctival infection group and the reproductive tract infection group, respectively. After constructing a PPI network and verifying the hub genes, CSF2, CD40, and CSF3 in the reproductive tract infection group proved to have considerable statistical significance. Conclusion In our research, the key genes in the biological process of reproductive tract infection with Chlamydia trachomatis were clarified through bioinformatics analysis. These hub genes may be further used in clinical treatment and clinical diagnosis.https://doi.org/10.1186/s40246-021-00313-8ConjunctivalReproductive tractChlamydia trachomatisBioinformatics analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guo-Dong Zhu Xun-Jie Cao Ya-Ping Li Jia-Xin Li Zi-Jian Leng Li-Min Xie Xu-Guang Guo |
spellingShingle |
Guo-Dong Zhu Xun-Jie Cao Ya-Ping Li Jia-Xin Li Zi-Jian Leng Li-Min Xie Xu-Guang Guo Identification of differentially expressed genes and signaling pathways in human conjunctiva and reproductive tract infected with Chlamydia trachomatis Human Genomics Conjunctival Reproductive tract Chlamydia trachomatis Bioinformatics analysis |
author_facet |
Guo-Dong Zhu Xun-Jie Cao Ya-Ping Li Jia-Xin Li Zi-Jian Leng Li-Min Xie Xu-Guang Guo |
author_sort |
Guo-Dong Zhu |
title |
Identification of differentially expressed genes and signaling pathways in human conjunctiva and reproductive tract infected with Chlamydia trachomatis |
title_short |
Identification of differentially expressed genes and signaling pathways in human conjunctiva and reproductive tract infected with Chlamydia trachomatis |
title_full |
Identification of differentially expressed genes and signaling pathways in human conjunctiva and reproductive tract infected with Chlamydia trachomatis |
title_fullStr |
Identification of differentially expressed genes and signaling pathways in human conjunctiva and reproductive tract infected with Chlamydia trachomatis |
title_full_unstemmed |
Identification of differentially expressed genes and signaling pathways in human conjunctiva and reproductive tract infected with Chlamydia trachomatis |
title_sort |
identification of differentially expressed genes and signaling pathways in human conjunctiva and reproductive tract infected with chlamydia trachomatis |
publisher |
BMC |
series |
Human Genomics |
issn |
1479-7364 |
publishDate |
2021-04-01 |
description |
Abstract Background Currently, Chlamydia trachomatis–specific host defense mechanisms in humans remain poorly defined. To study the characteristics of host cells infected early with Chlamydia trachomatis, we used bioinformatics methods to analyze the RNA transcription profiles of the conjunctiva, fallopian tubes, and endometrium in humans infected with Chlamydia trachomatis. Method The gene expression profiles of GSE20430, GSE20436, GSE26692, and GSE41075 were downloaded from the Gene Expression Synthesis (GEO) database. Then, we obtained the differentially expressed genes (DEGs) through the R 4.0.1 software. STRING was used to construct protein–protein interaction (PPI) networks; then, the Cytoscape 3.7.2 software was used to visualize the PPI and screen hub genes. GraphPad Prism 8.0 software was used to verify the expression of the hub gene. In addition, the gene–miRNA interaction was constructed on the NetworkAnalyst 3.0 platform using the miRTarBase v8.0 database. Results A total of 600 and 135 DEGs were screened out in the conjunctival infection group and the reproductive tract infection group, respectively. After constructing a PPI network and verifying the hub genes, CSF2, CD40, and CSF3 in the reproductive tract infection group proved to have considerable statistical significance. Conclusion In our research, the key genes in the biological process of reproductive tract infection with Chlamydia trachomatis were clarified through bioinformatics analysis. These hub genes may be further used in clinical treatment and clinical diagnosis. |
topic |
Conjunctival Reproductive tract Chlamydia trachomatis Bioinformatics analysis |
url |
https://doi.org/10.1186/s40246-021-00313-8 |
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