The design of the constructs of cpsD and simA genes of Streptococcus iniae
<p> </p><p class="NBMAbstractText"><span class="NBMAbstractPartsTitlesChar"><span style="line-height: 115%; font-size: 10.5pt;" lang="X-NONE">Background & Objective:</span></span><span lang="X-NONE"...
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Shahid Beheshti University of Medical Sciences
2014-04-01
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doaj-e670c7caa3dc4a7b8d6fe12367b232c42020-11-25T01:44:08ZengShahid Beheshti University of Medical SciencesNovelty in Biomedicine2345-33462345-39072014-04-0121154019The design of the constructs of cpsD and simA genes of Streptococcus iniaeMohsen Rahmanian Kooshkaki0Mojgan Bandehpour1Bahram Kazemi2Department of Biology, College of Basic Sciences, Tehran Science and Research Branch, Islamic Azad University, Tehran, IranBiotechnology Department,School of Medicine, Shahid Beheshti University of Medical SciencesBiotechnology Department,School of Medicine, Shahid Beheshti University of Medical Sciences<p> </p><p class="NBMAbstractText"><span class="NBMAbstractPartsTitlesChar"><span style="line-height: 115%; font-size: 10.5pt;" lang="X-NONE">Background & Objective:</span></span><span lang="X-NONE"> <em>Streptococcosis</em> is one of the bacterial infections in fish, especially rainbow trout which infects brain and nervous systems of fish and is caused by <em>S. iniae</em>. Estimation of the impact of disease prevalence by <em>S. iniae</em> in fish farming in some countries is reported about 100 million dollars per year. Some of the most effective proteins in pathogenicity of these bacteria are <em>SimA</em> and <em>CpsD</em>. In order to design new and effective vaccine, in this study cloning of two genes of Streptococcus was performed into <em>pNZ8148</em> vector and expressed in <em>Escherichia coli</em>. </span></p><p class="NBMAbstractText"><span class="NBMAbstractPartsTitlesChar"><span style="line-height: 115%; font-size: 10.5pt;" lang="X-NONE">Materials and Methods:</span></span><span lang="X-NONE"> <em>simA</em> and <em>cpsD</em> genes were subcloned into <em>pNZ8148</em> vector. Obtained constructs were transformed to expressing <em>E. coli BL21 </em>strain. After induction with nisin, SDS PAGE electrophoresis and Western blotting were used to confirm the procedures.</span></p><p class="NBMAbstractText"><span class="NBMAbstractPartsTitlesChar"><span style="line-height: 115%; font-size: 10.5pt;" lang="X-NONE">Results:</span></span><span lang="X-NONE"> Using PCR with specific and universal primers, the accuracy of cloning was confirmed. Final verification of expressed protein was carried out by SDS-PAGE and western blotting. </span></p><p class="NBMAbstractText"><span class="NBMAbstractPartsTitlesChar"><span style="line-height: 115%; font-size: 10.5pt;" lang="X-NONE">Conclusion:</span></span><span lang="X-NONE"> With regard to the obtained results, it seems that the generated gene construct in this study can be used as a vaccine against <em>Streptococcosis</em> in future researches. </span></p>http://journals.sbmu.ac.ir/index.php/nbm/article/view/6104Streptococcus iniae, cpsD gene, simA gene, pNZ8148 vector, cloning, Expression |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohsen Rahmanian Kooshkaki Mojgan Bandehpour Bahram Kazemi |
spellingShingle |
Mohsen Rahmanian Kooshkaki Mojgan Bandehpour Bahram Kazemi The design of the constructs of cpsD and simA genes of Streptococcus iniae Novelty in Biomedicine Streptococcus iniae, cpsD gene, simA gene, pNZ8148 vector, cloning, Expression |
author_facet |
Mohsen Rahmanian Kooshkaki Mojgan Bandehpour Bahram Kazemi |
author_sort |
Mohsen Rahmanian Kooshkaki |
title |
The design of the constructs of cpsD and simA genes of Streptococcus iniae |
title_short |
The design of the constructs of cpsD and simA genes of Streptococcus iniae |
title_full |
The design of the constructs of cpsD and simA genes of Streptococcus iniae |
title_fullStr |
The design of the constructs of cpsD and simA genes of Streptococcus iniae |
title_full_unstemmed |
The design of the constructs of cpsD and simA genes of Streptococcus iniae |
title_sort |
design of the constructs of cpsd and sima genes of streptococcus iniae |
publisher |
Shahid Beheshti University of Medical Sciences |
series |
Novelty in Biomedicine |
issn |
2345-3346 2345-3907 |
publishDate |
2014-04-01 |
description |
<p> </p><p class="NBMAbstractText"><span class="NBMAbstractPartsTitlesChar"><span style="line-height: 115%; font-size: 10.5pt;" lang="X-NONE">Background & Objective:</span></span><span lang="X-NONE"> <em>Streptococcosis</em> is one of the bacterial infections in fish, especially rainbow trout which infects brain and nervous systems of fish and is caused by <em>S. iniae</em>. Estimation of the impact of disease prevalence by <em>S. iniae</em> in fish farming in some countries is reported about 100 million dollars per year. Some of the most effective proteins in pathogenicity of these bacteria are <em>SimA</em> and <em>CpsD</em>. In order to design new and effective vaccine, in this study cloning of two genes of Streptococcus was performed into <em>pNZ8148</em> vector and expressed in <em>Escherichia coli</em>. </span></p><p class="NBMAbstractText"><span class="NBMAbstractPartsTitlesChar"><span style="line-height: 115%; font-size: 10.5pt;" lang="X-NONE">Materials and Methods:</span></span><span lang="X-NONE"> <em>simA</em> and <em>cpsD</em> genes were subcloned into <em>pNZ8148</em> vector. Obtained constructs were transformed to expressing <em>E. coli BL21 </em>strain. After induction with nisin, SDS PAGE electrophoresis and Western blotting were used to confirm the procedures.</span></p><p class="NBMAbstractText"><span class="NBMAbstractPartsTitlesChar"><span style="line-height: 115%; font-size: 10.5pt;" lang="X-NONE">Results:</span></span><span lang="X-NONE"> Using PCR with specific and universal primers, the accuracy of cloning was confirmed. Final verification of expressed protein was carried out by SDS-PAGE and western blotting. </span></p><p class="NBMAbstractText"><span class="NBMAbstractPartsTitlesChar"><span style="line-height: 115%; font-size: 10.5pt;" lang="X-NONE">Conclusion:</span></span><span lang="X-NONE"> With regard to the obtained results, it seems that the generated gene construct in this study can be used as a vaccine against <em>Streptococcosis</em> in future researches. </span></p> |
topic |
Streptococcus iniae, cpsD gene, simA gene, pNZ8148 vector, cloning, Expression |
url |
http://journals.sbmu.ac.ir/index.php/nbm/article/view/6104 |
work_keys_str_mv |
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