Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in <i>Caenorhabditis elegans</i>

Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in <i>Caenorhabditis elegans</i>

Non-nutritive sweeteners are widely used in food and medicines to reduce energy content without compromising flavor. Herein, we report that Rebaudioside A (Reb A), a natural, non-nutritive sweetener, can extend both the lifespan and healthspan of <i>C. elegans</i>. The beneficial effects...

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Bibliographic Details
Main Authors: Pan Li, Zehua Wang, Sin Man Lam, Guanghou Shui
Format: Article
Language:English
Published: MDPI AG 2021-02-01
Series:Antioxidants
Subjects:
<i>C. elegans</i>
non-nutritive sweetener
rebaudioside A
aging
oxidative stress resistance
TOR
Online Access:https://www.mdpi.com/2076-3921/10/2/262
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spelling doaj-e673753f548e4317a7a191b375027de02021-02-09T00:05:22ZengMDPI AGAntioxidants2076-39212021-02-011026226210.3390/antiox10020262Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in <i>Caenorhabditis elegans</i>Pan Li0Zehua Wang1Sin Man Lam2Guanghou Shui3State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaNon-nutritive sweeteners are widely used in food and medicines to reduce energy content without compromising flavor. Herein, we report that Rebaudioside A (Reb A), a natural, non-nutritive sweetener, can extend both the lifespan and healthspan of <i>C. elegans</i>. The beneficial effects of Reb A were principally mediated via reducing the level of cellular reactive oxygen species (ROS) in response to oxidative stress and attenuating neutral lipid accumulation with aging. Transcriptomics analysis presented maximum differential expression of genes along the target of rapamycin (TOR) signaling pathway, which was further confirmed by quantitative real-time PCR (qPCR); while lipidomics uncovered concomitant reductions in the levels of phosphatidic acids (PAs), phosphatidylinositols (PIs) and lysophosphatidylcholines (LPCs) in worms treated with Reb A. Our results suggest that Reb A attenuates aging by acting as effective cellular antioxidants and also in lowering the ectopic accumulation of neutral lipids.https://www.mdpi.com/2076-3921/10/2/262<i>C. elegans</i>non-nutritive sweetenerrebaudioside Aagingoxidative stress resistanceTOR
collection DOAJ
language English
format Article
sources DOAJ
author Pan Li
Zehua Wang
Sin Man Lam
Guanghou Shui
spellingShingle Pan Li
Zehua Wang
Sin Man Lam
Guanghou Shui
Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in <i>Caenorhabditis elegans</i>
Antioxidants
<i>C. elegans</i>
non-nutritive sweetener
rebaudioside A
aging
oxidative stress resistance
TOR
author_facet Pan Li
Zehua Wang
Sin Man Lam
Guanghou Shui
author_sort Pan Li
title Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in <i>Caenorhabditis elegans</i>
title_short Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in <i>Caenorhabditis elegans</i>
title_full Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in <i>Caenorhabditis elegans</i>
title_fullStr Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in <i>Caenorhabditis elegans</i>
title_full_unstemmed Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in <i>Caenorhabditis elegans</i>
title_sort rebaudioside a enhances resistance to oxidative stress and extends lifespan and healthspan in <i>caenorhabditis elegans</i>
publisher MDPI AG
series Antioxidants
issn 2076-3921
publishDate 2021-02-01
description Non-nutritive sweeteners are widely used in food and medicines to reduce energy content without compromising flavor. Herein, we report that Rebaudioside A (Reb A), a natural, non-nutritive sweetener, can extend both the lifespan and healthspan of <i>C. elegans</i>. The beneficial effects of Reb A were principally mediated via reducing the level of cellular reactive oxygen species (ROS) in response to oxidative stress and attenuating neutral lipid accumulation with aging. Transcriptomics analysis presented maximum differential expression of genes along the target of rapamycin (TOR) signaling pathway, which was further confirmed by quantitative real-time PCR (qPCR); while lipidomics uncovered concomitant reductions in the levels of phosphatidic acids (PAs), phosphatidylinositols (PIs) and lysophosphatidylcholines (LPCs) in worms treated with Reb A. Our results suggest that Reb A attenuates aging by acting as effective cellular antioxidants and also in lowering the ectopic accumulation of neutral lipids.
topic <i>C. elegans</i>
non-nutritive sweetener
rebaudioside A
aging
oxidative stress resistance
TOR
url https://www.mdpi.com/2076-3921/10/2/262
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AT sinmanlam rebaudiosideaenhancesresistancetooxidativestressandextendslifespanandhealthspaninicaenorhabditiselegansi
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