Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in <i>Caenorhabditis elegans</i>
Non-nutritive sweeteners are widely used in food and medicines to reduce energy content without compromising flavor. Herein, we report that Rebaudioside A (Reb A), a natural, non-nutritive sweetener, can extend both the lifespan and healthspan of <i>C. elegans</i>. The beneficial effects...
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doaj-e673753f548e4317a7a191b375027de02021-02-09T00:05:22ZengMDPI AGAntioxidants2076-39212021-02-011026226210.3390/antiox10020262Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in <i>Caenorhabditis elegans</i>Pan Li0Zehua Wang1Sin Man Lam2Guanghou Shui3State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaNon-nutritive sweeteners are widely used in food and medicines to reduce energy content without compromising flavor. Herein, we report that Rebaudioside A (Reb A), a natural, non-nutritive sweetener, can extend both the lifespan and healthspan of <i>C. elegans</i>. The beneficial effects of Reb A were principally mediated via reducing the level of cellular reactive oxygen species (ROS) in response to oxidative stress and attenuating neutral lipid accumulation with aging. Transcriptomics analysis presented maximum differential expression of genes along the target of rapamycin (TOR) signaling pathway, which was further confirmed by quantitative real-time PCR (qPCR); while lipidomics uncovered concomitant reductions in the levels of phosphatidic acids (PAs), phosphatidylinositols (PIs) and lysophosphatidylcholines (LPCs) in worms treated with Reb A. Our results suggest that Reb A attenuates aging by acting as effective cellular antioxidants and also in lowering the ectopic accumulation of neutral lipids.https://www.mdpi.com/2076-3921/10/2/262<i>C. elegans</i>non-nutritive sweetenerrebaudioside Aagingoxidative stress resistanceTOR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pan Li Zehua Wang Sin Man Lam Guanghou Shui |
spellingShingle |
Pan Li Zehua Wang Sin Man Lam Guanghou Shui Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in <i>Caenorhabditis elegans</i> Antioxidants <i>C. elegans</i> non-nutritive sweetener rebaudioside A aging oxidative stress resistance TOR |
author_facet |
Pan Li Zehua Wang Sin Man Lam Guanghou Shui |
author_sort |
Pan Li |
title |
Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in <i>Caenorhabditis elegans</i> |
title_short |
Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in <i>Caenorhabditis elegans</i> |
title_full |
Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in <i>Caenorhabditis elegans</i> |
title_fullStr |
Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in <i>Caenorhabditis elegans</i> |
title_full_unstemmed |
Rebaudioside A Enhances Resistance to Oxidative Stress and Extends Lifespan and Healthspan in <i>Caenorhabditis elegans</i> |
title_sort |
rebaudioside a enhances resistance to oxidative stress and extends lifespan and healthspan in <i>caenorhabditis elegans</i> |
publisher |
MDPI AG |
series |
Antioxidants |
issn |
2076-3921 |
publishDate |
2021-02-01 |
description |
Non-nutritive sweeteners are widely used in food and medicines to reduce energy content without compromising flavor. Herein, we report that Rebaudioside A (Reb A), a natural, non-nutritive sweetener, can extend both the lifespan and healthspan of <i>C. elegans</i>. The beneficial effects of Reb A were principally mediated via reducing the level of cellular reactive oxygen species (ROS) in response to oxidative stress and attenuating neutral lipid accumulation with aging. Transcriptomics analysis presented maximum differential expression of genes along the target of rapamycin (TOR) signaling pathway, which was further confirmed by quantitative real-time PCR (qPCR); while lipidomics uncovered concomitant reductions in the levels of phosphatidic acids (PAs), phosphatidylinositols (PIs) and lysophosphatidylcholines (LPCs) in worms treated with Reb A. Our results suggest that Reb A attenuates aging by acting as effective cellular antioxidants and also in lowering the ectopic accumulation of neutral lipids. |
topic |
<i>C. elegans</i> non-nutritive sweetener rebaudioside A aging oxidative stress resistance TOR |
url |
https://www.mdpi.com/2076-3921/10/2/262 |
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