Sperm Parameters, ASAs and Apoptosis After Processing by the Double Tube and Swim up Methods
The aim of this study was to improve the quality of semen samples by using a novel double-tube (DT) method. The DT method was developed to select sperm and compared with traditional swim-up (SU) technique for 31 semen samples. Sperm DNA integrity were tested with TUNEL and SCSA. Content of antisperm...
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doaj-e68d3b27d0f34ba18992d34b5607d1ef2021-03-24T22:05:02ZengSAGE PublishingAmerican Journal of Men's Health1557-98912021-03-011510.1177/15579883211001202Sperm Parameters, ASAs and Apoptosis After Processing by the Double Tube and Swim up MethodsZhi-Da Shi0Yan-Ping Zhang1Li-Ping Zhai2Mei-Hua Zhang3Yun-Ling Dong4Hui-Jun Yang5Yi Qiu6Key Laboratory of Birth Regulation and Control Technology of National Health Commission of China, Key Laboratory for Improving Birth Outcome Technique, Maternal and Child Health Care Hospital of Shandong Province, Jinan, Shandong, People’s Republic of ChinaKey Laboratory of Birth Regulation and Control Technology of National Health Commission of China, Key Laboratory for Improving Birth Outcome Technique, Maternal and Child Health Care Hospital of Shandong Province, Jinan, Shandong, People’s Republic of ChinaShandong Provincial Institute of Control of Endemic Disease, Jinan, Shandong, People’s Republic of ChinaKey Laboratory of Birth Regulation and Control Technology of National Health Commission of China, Key Laboratory for Improving Birth Outcome Technique, Maternal and Child Health Care Hospital of Shandong Province, Jinan, Shandong, People’s Republic of ChinaKey Laboratory of Birth Regulation and Control Technology of National Health Commission of China, Key Laboratory for Improving Birth Outcome Technique, Maternal and Child Health Care Hospital of Shandong Province, Jinan, Shandong, People’s Republic of ChinaKey Laboratory of Birth Regulation and Control Technology of National Health Commission of China, Key Laboratory for Improving Birth Outcome Technique, Maternal and Child Health Care Hospital of Shandong Province, Jinan, Shandong, People’s Republic of ChinaKey Laboratory of Birth Regulation and Control Technology of National Health Commission of China, Key Laboratory for Improving Birth Outcome Technique, Maternal and Child Health Care Hospital of Shandong Province, Jinan, Shandong, People’s Republic of ChinaThe aim of this study was to improve the quality of semen samples by using a novel double-tube (DT) method. The DT method was developed to select sperm and compared with traditional swim-up (SU) technique for 31 semen samples. Sperm DNA integrity were tested with TUNEL and SCSA. Content of antisperm antibodies (ASA) in the semen was measured by ELISA and MAR. Levels of the caspase-3 in the sperm were assessed by western blotting. After SU and DT, 15 couples and 16 couples were underwent IVF-ET. The number of RCDs, the percentage of SDF and DFI, ASA and the level of caspase-3 were significantly decreased after DT and SU ( p = .001 and p < .001). When the DT and SU compared, there were significant changes in the number of RCD, the percentage of SDF and DFI, ASA and the level of caspase-3 ( p < 0.05-0.001). There was a higher cleavage rate ( p = .017) and a lower abortion rate ( p < .05) in DT-IVF group than in SU-IVF group. DT selection yielded spermatozoa with low RCDs, DFI, ASA, and caspase-3 which would be benefit for ART.https://doi.org/10.1177/15579883211001202 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhi-Da Shi Yan-Ping Zhang Li-Ping Zhai Mei-Hua Zhang Yun-Ling Dong Hui-Jun Yang Yi Qiu |
spellingShingle |
Zhi-Da Shi Yan-Ping Zhang Li-Ping Zhai Mei-Hua Zhang Yun-Ling Dong Hui-Jun Yang Yi Qiu Sperm Parameters, ASAs and Apoptosis After Processing by the Double Tube and Swim up Methods American Journal of Men's Health |
author_facet |
Zhi-Da Shi Yan-Ping Zhang Li-Ping Zhai Mei-Hua Zhang Yun-Ling Dong Hui-Jun Yang Yi Qiu |
author_sort |
Zhi-Da Shi |
title |
Sperm Parameters, ASAs and Apoptosis After Processing by the Double Tube and Swim up Methods |
title_short |
Sperm Parameters, ASAs and Apoptosis After Processing by the Double Tube and Swim up Methods |
title_full |
Sperm Parameters, ASAs and Apoptosis After Processing by the Double Tube and Swim up Methods |
title_fullStr |
Sperm Parameters, ASAs and Apoptosis After Processing by the Double Tube and Swim up Methods |
title_full_unstemmed |
Sperm Parameters, ASAs and Apoptosis After Processing by the Double Tube and Swim up Methods |
title_sort |
sperm parameters, asas and apoptosis after processing by the double tube and swim up methods |
publisher |
SAGE Publishing |
series |
American Journal of Men's Health |
issn |
1557-9891 |
publishDate |
2021-03-01 |
description |
The aim of this study was to improve the quality of semen samples by using a novel double-tube (DT) method. The DT method was developed to select sperm and compared with traditional swim-up (SU) technique for 31 semen samples. Sperm DNA integrity were tested with TUNEL and SCSA. Content of antisperm antibodies (ASA) in the semen was measured by ELISA and MAR. Levels of the caspase-3 in the sperm were assessed by western blotting. After SU and DT, 15 couples and 16 couples were underwent IVF-ET. The number of RCDs, the percentage of SDF and DFI, ASA and the level of caspase-3 were significantly decreased after DT and SU ( p = .001 and p < .001). When the DT and SU compared, there were significant changes in the number of RCD, the percentage of SDF and DFI, ASA and the level of caspase-3 ( p < 0.05-0.001). There was a higher cleavage rate ( p = .017) and a lower abortion rate ( p < .05) in DT-IVF group than in SU-IVF group. DT selection yielded spermatozoa with low RCDs, DFI, ASA, and caspase-3 which would be benefit for ART. |
url |
https://doi.org/10.1177/15579883211001202 |
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