International lineages of Salmonella enterica serovars isolated from chicken farms, Wakiso District, Uganda.
The growing occurrence of multidrug-resistant (MDR) Salmonella enterica in poultry has been reported with public health concern worldwide. We reported, recently, the occurrence of Escherichia coli and Salmonella enterica serovars carrying clinically relevant resistance genes in dairy cattle farms in...
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doaj-e6c788bc47f9406cabcd80c2b96e86252021-03-04T12:43:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01151e022048410.1371/journal.pone.0220484International lineages of Salmonella enterica serovars isolated from chicken farms, Wakiso District, Uganda.Takiyah BallDaniel MonteAwa Aidara-KaneJorge MatheuHongyu RuSiddhartha ThakurFrancis EjobiPaula Fedorka-CrayThe growing occurrence of multidrug-resistant (MDR) Salmonella enterica in poultry has been reported with public health concern worldwide. We reported, recently, the occurrence of Escherichia coli and Salmonella enterica serovars carrying clinically relevant resistance genes in dairy cattle farms in the Wakiso District, Uganda, highlighting an urgent need to monitor food-producing animal environments. Here, we present the prevalence, antimicrobial resistance, and sequence type of 51 Salmonella isolates recovered from 379 environmental samples from chicken farms in Uganda. Among the Salmonella isolates, 32/51 (62.7%) were resistant to at least one antimicrobial, and 10/51 (19.6%) displayed multiple drug resistance. Through PCR, five replicon plasmids were identified among chicken Salmonella isolates including IncFIIS 17/51 (33.3%), IncI1α 12/51 (23.5%), IncP 8/51 (15.7%), IncX1 8/51 (15.7%), and IncX2 1/51 (2.0%). In addition, we identified two additional replicons through WGS (Whole Genome Sequencing; ColpVC and IncFIB). A significant seasonal difference between chicken sampling periods was observed (p = 0.0017). We conclude that MDR Salmonella highlights the risks posed to animals and humans. Implementing a robust, integrated surveillance system will aid in monitoring MDR zoonotic threats.https://doi.org/10.1371/journal.pone.0220484 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Takiyah Ball Daniel Monte Awa Aidara-Kane Jorge Matheu Hongyu Ru Siddhartha Thakur Francis Ejobi Paula Fedorka-Cray |
spellingShingle |
Takiyah Ball Daniel Monte Awa Aidara-Kane Jorge Matheu Hongyu Ru Siddhartha Thakur Francis Ejobi Paula Fedorka-Cray International lineages of Salmonella enterica serovars isolated from chicken farms, Wakiso District, Uganda. PLoS ONE |
author_facet |
Takiyah Ball Daniel Monte Awa Aidara-Kane Jorge Matheu Hongyu Ru Siddhartha Thakur Francis Ejobi Paula Fedorka-Cray |
author_sort |
Takiyah Ball |
title |
International lineages of Salmonella enterica serovars isolated from chicken farms, Wakiso District, Uganda. |
title_short |
International lineages of Salmonella enterica serovars isolated from chicken farms, Wakiso District, Uganda. |
title_full |
International lineages of Salmonella enterica serovars isolated from chicken farms, Wakiso District, Uganda. |
title_fullStr |
International lineages of Salmonella enterica serovars isolated from chicken farms, Wakiso District, Uganda. |
title_full_unstemmed |
International lineages of Salmonella enterica serovars isolated from chicken farms, Wakiso District, Uganda. |
title_sort |
international lineages of salmonella enterica serovars isolated from chicken farms, wakiso district, uganda. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2020-01-01 |
description |
The growing occurrence of multidrug-resistant (MDR) Salmonella enterica in poultry has been reported with public health concern worldwide. We reported, recently, the occurrence of Escherichia coli and Salmonella enterica serovars carrying clinically relevant resistance genes in dairy cattle farms in the Wakiso District, Uganda, highlighting an urgent need to monitor food-producing animal environments. Here, we present the prevalence, antimicrobial resistance, and sequence type of 51 Salmonella isolates recovered from 379 environmental samples from chicken farms in Uganda. Among the Salmonella isolates, 32/51 (62.7%) were resistant to at least one antimicrobial, and 10/51 (19.6%) displayed multiple drug resistance. Through PCR, five replicon plasmids were identified among chicken Salmonella isolates including IncFIIS 17/51 (33.3%), IncI1α 12/51 (23.5%), IncP 8/51 (15.7%), IncX1 8/51 (15.7%), and IncX2 1/51 (2.0%). In addition, we identified two additional replicons through WGS (Whole Genome Sequencing; ColpVC and IncFIB). A significant seasonal difference between chicken sampling periods was observed (p = 0.0017). We conclude that MDR Salmonella highlights the risks posed to animals and humans. Implementing a robust, integrated surveillance system will aid in monitoring MDR zoonotic threats. |
url |
https://doi.org/10.1371/journal.pone.0220484 |
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