How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways
Abstract As recorded in Traditional Chinese Medicine (TCM) theory, Gancao (Glycyrrhizae Radix et Rhizoma) could weaken the pharmacological effect or increase the toxicity of Yuanhua (Genkwa Flos). However, the theory has been suspected due to lack of evidence. Here, we investigate whether Gancao cou...
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Format: | Article |
Language: | English |
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Nature Publishing Group
2017-06-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-017-03201-1 |
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doaj-e70c3671188347a5978c6aaba1951147 |
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record_format |
Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jin-gao Yu Jianming Guo Kevin Yue Zhu Weiwei Tao Yanyan Chen Pei Liu Yongqing Hua Yuping Tang Jin-ao Duan |
spellingShingle |
Jin-gao Yu Jianming Guo Kevin Yue Zhu Weiwei Tao Yanyan Chen Pei Liu Yongqing Hua Yuping Tang Jin-ao Duan How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways Scientific Reports |
author_facet |
Jin-gao Yu Jianming Guo Kevin Yue Zhu Weiwei Tao Yanyan Chen Pei Liu Yongqing Hua Yuping Tang Jin-ao Duan |
author_sort |
Jin-gao Yu |
title |
How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways |
title_short |
How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways |
title_full |
How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways |
title_fullStr |
How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways |
title_full_unstemmed |
How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathways |
title_sort |
how impaired efficacy happened between gancao and yuanhua: compounds, targets and pathways |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-06-01 |
description |
Abstract As recorded in Traditional Chinese Medicine (TCM) theory, Gancao (Glycyrrhizae Radix et Rhizoma) could weaken the pharmacological effect or increase the toxicity of Yuanhua (Genkwa Flos). However, the theory has been suspected due to lack of evidence. Here, we investigate whether Gancao could weaken Yuanhua’s diuretic effect, if so, which chemicals and which targets may be involved. Results showed that Yuanhua exerted diuretic effect through down-regulating renal AQP 2, without electrolyte disturbances such as K+ loss which has been observed as side-effect of most diuretics. Gancao had no diuretic effect, but could impair Yuanhua’s diuretic effect through up-regulating renal AQP 2. Glycyrrhetinic acid (GRA) in Gancao could up-regulate AQP 2 and counteract the AQP 2 regulation effect of Yuanhuacine (YHC) and Ginkwanin (GKW) in Yuanhua. Network pharmacology method suggested that YHC, GKW and GRA could bind to MEK1/FGFR1 protein and influence ERK-MAPK pathway, which was verified by Western blotting. This study supports TCM theory and reminds that more attention should be paid to the safety and efficacy problems induced by improper combination between herbs. Moreover, we suggested that promising diuretics with less side effects can be developed from Chinese Medicines such as Yuanhua. |
url |
https://doi.org/10.1038/s41598-017-03201-1 |
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doaj-e70c3671188347a5978c6aaba19511472020-12-08T00:50:27ZengNature Publishing GroupScientific Reports2045-23222017-06-017111310.1038/s41598-017-03201-1How impaired efficacy happened between Gancao and Yuanhua: Compounds, targets and pathwaysJin-gao Yu0Jianming Guo1Kevin Yue Zhu2Weiwei Tao3Yanyan Chen4Pei Liu5Yongqing Hua6Yuping Tang7Jin-ao Duan8Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese MedicineJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese MedicineJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese MedicineJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese MedicineJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese MedicineJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese MedicineJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese MedicineJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese MedicineJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese MedicineAbstract As recorded in Traditional Chinese Medicine (TCM) theory, Gancao (Glycyrrhizae Radix et Rhizoma) could weaken the pharmacological effect or increase the toxicity of Yuanhua (Genkwa Flos). However, the theory has been suspected due to lack of evidence. Here, we investigate whether Gancao could weaken Yuanhua’s diuretic effect, if so, which chemicals and which targets may be involved. Results showed that Yuanhua exerted diuretic effect through down-regulating renal AQP 2, without electrolyte disturbances such as K+ loss which has been observed as side-effect of most diuretics. Gancao had no diuretic effect, but could impair Yuanhua’s diuretic effect through up-regulating renal AQP 2. Glycyrrhetinic acid (GRA) in Gancao could up-regulate AQP 2 and counteract the AQP 2 regulation effect of Yuanhuacine (YHC) and Ginkwanin (GKW) in Yuanhua. Network pharmacology method suggested that YHC, GKW and GRA could bind to MEK1/FGFR1 protein and influence ERK-MAPK pathway, which was verified by Western blotting. This study supports TCM theory and reminds that more attention should be paid to the safety and efficacy problems induced by improper combination between herbs. Moreover, we suggested that promising diuretics with less side effects can be developed from Chinese Medicines such as Yuanhua.https://doi.org/10.1038/s41598-017-03201-1 |