Transcriptional outcomes and kinetic patterning of gene expression in response to NF-κB activation.

• Main Authors: Mingming Zhao, Jaimy Joy, Weiqiang Zhou, Supriyo De, William H Wood, Kevin G Becker, Hongkai Ji, Ranjan Sen
• Format: Article
• Language: English
• Published: Public Library of Science (PLoS) 2018-09-01
• Series: PLoS Biology
• Online Access: https://doi.org/10.1371/journal.pbio.2006347

Transcription factor nuclear factor kappa B (NF-κB) regulates cellular responses to environmental cues. Many stimuli induce NF-κB transiently, making time-dependent transcriptional outputs a fundamental feature of NF-κB activation. Here we show that NF-κB target genes have distinct kinetic patterns in activated B lymphoma cells. By combining RELA binding, RNA polymerase II (Pol II) recruitment, and perturbation of NF-κB activation, we demonstrate that kinetic differences amongst early- and late-activated RELA target genes can be understood based on chromatin configuration prior to cell activation and RELA-dependent priming, respectively. We also identified genes that were repressed by RELA activation and others that responded to RELA-activated transcription factors. Cumulatively, our studies define an NF-κB-responsive inducible gene cascade in activated B cells.