LigasureTM Seals Small Lymphatic Vessels as Comparably Well as Hem-o-lok® Clips

Objective:The purpose of this study is to compare small lymphatic vessel sealing using LigasureTM (LS) and Hem-o-lok® (HML) clips in an ex vivo canine spermatic cord model.Materials and Methods:Canine testes with spermatic cord attached were harvested. By random distribution, the spermatic cord of e...

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Main Authors: Dwayne Chang, Isaac Thyer
Format: Article
Language:English
Published: Galenos Yayinevi 2020-12-01
Series:Journal of Urological Surgery
Subjects:
Online Access: http://jurolsurgery.org/archives/archive-detail/article-preview/ligasuresuptm-sup-seals-small-lymphatic-vessels-as/42819
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spelling doaj-e71704ee70474c7b962c89eae2c657412021-01-08T10:54:23ZengGalenos YayineviJournal of Urological Surgery2148-95802148-95802020-12-017428128410.4274/jus.galenos.2020.335913049054LigasureTM Seals Small Lymphatic Vessels as Comparably Well as Hem-o-lok® ClipsDwayne Chang0Isaac Thyer1 Hollywood Private Hospital, Clinic of Urology, Nedlands, Australia Royal Perth Hospital, Clinic of Urology, Perth, Australia Objective:The purpose of this study is to compare small lymphatic vessel sealing using LigasureTM (LS) and Hem-o-lok® (HML) clips in an ex vivo canine spermatic cord model.Materials and Methods:Canine testes with spermatic cord attached were harvested. By random distribution, the spermatic cord of each testis was either (i) sealed with LS, (ii) clipped with one HML clip, or (iii) had no intervention. The testis was then injected with a Patent Blue V dye solution. At 5 minutes and 12 hours after injection, the distal spermatic cord was inspected for macroscopically visible dye. One random spermatic cord from each of the LS and HML cohorts free of macroscopic evidence of dye was examined using the frozen section histology of the cross sections proximal and distal to the intervention site to detect the microscopic presence of dye.Results:During the study period, 18 canine testes were harvested. After randomization, five, six, and seven testes were included in the control, HML, and LS groups, respectively. In the control group, all 5 testes had leaked blue dye from the cut end of the spermatic cord at 5 minutes. Further, in the HML group, dye was macroscopically visible distal to the intervention site (intervention failure) in 2 out of 6 cases at 5 minutes. In the LS group, 2 out of 7 testes had leaked blue dye at 5 minutes. All testes that did not fail at 5 minutes also did not fail at 12 hours in both LS and HML groups.Conclusion:LS is an effective alternative option to HML clips to seal small lymphatic vessels. http://jurolsurgery.org/archives/archive-detail/article-preview/ligasuresuptm-sup-seals-small-lymphatic-vessels-as/42819 diathermylymphedemalymph nodessurgical instruments
collection DOAJ
language English
format Article
sources DOAJ
author Dwayne Chang
Isaac Thyer
spellingShingle Dwayne Chang
Isaac Thyer
LigasureTM Seals Small Lymphatic Vessels as Comparably Well as Hem-o-lok® Clips
Journal of Urological Surgery
diathermy
lymphedema
lymph nodes
surgical instruments
author_facet Dwayne Chang
Isaac Thyer
author_sort Dwayne Chang
title LigasureTM Seals Small Lymphatic Vessels as Comparably Well as Hem-o-lok® Clips
title_short LigasureTM Seals Small Lymphatic Vessels as Comparably Well as Hem-o-lok® Clips
title_full LigasureTM Seals Small Lymphatic Vessels as Comparably Well as Hem-o-lok® Clips
title_fullStr LigasureTM Seals Small Lymphatic Vessels as Comparably Well as Hem-o-lok® Clips
title_full_unstemmed LigasureTM Seals Small Lymphatic Vessels as Comparably Well as Hem-o-lok® Clips
title_sort ligasuretm seals small lymphatic vessels as comparably well as hem-o-lok® clips
publisher Galenos Yayinevi
series Journal of Urological Surgery
issn 2148-9580
2148-9580
publishDate 2020-12-01
description Objective:The purpose of this study is to compare small lymphatic vessel sealing using LigasureTM (LS) and Hem-o-lok® (HML) clips in an ex vivo canine spermatic cord model.Materials and Methods:Canine testes with spermatic cord attached were harvested. By random distribution, the spermatic cord of each testis was either (i) sealed with LS, (ii) clipped with one HML clip, or (iii) had no intervention. The testis was then injected with a Patent Blue V dye solution. At 5 minutes and 12 hours after injection, the distal spermatic cord was inspected for macroscopically visible dye. One random spermatic cord from each of the LS and HML cohorts free of macroscopic evidence of dye was examined using the frozen section histology of the cross sections proximal and distal to the intervention site to detect the microscopic presence of dye.Results:During the study period, 18 canine testes were harvested. After randomization, five, six, and seven testes were included in the control, HML, and LS groups, respectively. In the control group, all 5 testes had leaked blue dye from the cut end of the spermatic cord at 5 minutes. Further, in the HML group, dye was macroscopically visible distal to the intervention site (intervention failure) in 2 out of 6 cases at 5 minutes. In the LS group, 2 out of 7 testes had leaked blue dye at 5 minutes. All testes that did not fail at 5 minutes also did not fail at 12 hours in both LS and HML groups.Conclusion:LS is an effective alternative option to HML clips to seal small lymphatic vessels.
topic diathermy
lymphedema
lymph nodes
surgical instruments
url http://jurolsurgery.org/archives/archive-detail/article-preview/ligasuresuptm-sup-seals-small-lymphatic-vessels-as/42819
work_keys_str_mv AT dwaynechang ligasuretmsealssmalllymphaticvesselsascomparablywellashemolokclips
AT isaacthyer ligasuretmsealssmalllymphaticvesselsascomparablywellashemolokclips
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