Low dietary fish-oil threshold for myocardial membrane n-3 PUFA enrichment independent of n-6 PUFA intake in rats
Long chain n-3 PUFA docosahexaenoic acid (DHA) is important for heart and brain function. Investigations of biologically plausible mechanisms using animal models associate cardioprotection with DHA incorporation into myocardial membranes that are largely derived from supra-physiological fish oil (FO...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2010-07-01
|
Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520371066 |
id |
doaj-e723262d3950410ebcf815814a2a41e6 |
---|---|
record_format |
Article |
spelling |
doaj-e723262d3950410ebcf815814a2a41e62021-04-28T06:01:54ZengElsevierJournal of Lipid Research0022-22752010-07-0151718411848Low dietary fish-oil threshold for myocardial membrane n-3 PUFA enrichment independent of n-6 PUFA intake in ratsEmily L. Slee0Peter L. McLennan1Alice J. Owen2Mandy L. Theiss3School of Health Sciences, University of Wollongong, Wollongong, AustraliaTo whom correspondence should be addressed; Graduate School of Medicine, University of Wollongong, Wollongong, AustraliaSmart Foods Centre, University of Wollongong, Wollongong, Australia; Department of Epidemiology and Preventive Medicine, Monash University, Prahran, AustraliaSchool of Health Sciences, University of Wollongong, Wollongong, AustraliaLong chain n-3 PUFA docosahexaenoic acid (DHA) is important for heart and brain function. Investigations of biologically plausible mechanisms using animal models associate cardioprotection with DHA incorporation into myocardial membranes that are largely derived from supra-physiological fish oil (FO) intake. We measured the incorporation of DHA into myocardial membranes of rats from low dietary FO intake within human dietary range and quantitatively assessed the influence of dietary n-6 PUFA. With rats fed diets containing 0.16%–5% FO, equal to 0.12%–8.7% energy (%en) as eicosapentaenoic acid (EPA) and DHA (EPA+DHA), and either 1.5%en or 7.5%en n-6 PUFA (linoleic acid) for four weeks, dietary n-6:n-3 PUFA ratios ranged from 74 to 0.3. Myocardial DHA concentration increased in a log-linear fashion with a dietary threshold of 0.019%en as EPA+DHA and half maximal dietary [EPA+DHA] equal to 0.29%en (95% CI, 0.23–0.35). Dietary linoleic acid intake did not influence myocardial DHA. Myocardial membranes are sensitive to absolute dietary intake of long chain n-3 PUFA at low %en in the rat, equivalent to a human intake of one meal of fatty fish per week or less. The dietary ratio of n-6:n-3 PUFA has no influence on long chain n-3 PUFA cellular incorporation from dietary fish oil.http://www.sciencedirect.com/science/article/pii/S0022227520371066myocardial membranedietary lipiddocosahexaenoic acidomega-3 fatty acidpolyunsaturated fatty acidn-6:n-3 PUFA ratio |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Emily L. Slee Peter L. McLennan Alice J. Owen Mandy L. Theiss |
spellingShingle |
Emily L. Slee Peter L. McLennan Alice J. Owen Mandy L. Theiss Low dietary fish-oil threshold for myocardial membrane n-3 PUFA enrichment independent of n-6 PUFA intake in rats Journal of Lipid Research myocardial membrane dietary lipid docosahexaenoic acid omega-3 fatty acid polyunsaturated fatty acid n-6:n-3 PUFA ratio |
author_facet |
Emily L. Slee Peter L. McLennan Alice J. Owen Mandy L. Theiss |
author_sort |
Emily L. Slee |
title |
Low dietary fish-oil threshold for myocardial membrane n-3 PUFA enrichment independent of n-6 PUFA intake in rats |
title_short |
Low dietary fish-oil threshold for myocardial membrane n-3 PUFA enrichment independent of n-6 PUFA intake in rats |
title_full |
Low dietary fish-oil threshold for myocardial membrane n-3 PUFA enrichment independent of n-6 PUFA intake in rats |
title_fullStr |
Low dietary fish-oil threshold for myocardial membrane n-3 PUFA enrichment independent of n-6 PUFA intake in rats |
title_full_unstemmed |
Low dietary fish-oil threshold for myocardial membrane n-3 PUFA enrichment independent of n-6 PUFA intake in rats |
title_sort |
low dietary fish-oil threshold for myocardial membrane n-3 pufa enrichment independent of n-6 pufa intake in rats |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2010-07-01 |
description |
Long chain n-3 PUFA docosahexaenoic acid (DHA) is important for heart and brain function. Investigations of biologically plausible mechanisms using animal models associate cardioprotection with DHA incorporation into myocardial membranes that are largely derived from supra-physiological fish oil (FO) intake. We measured the incorporation of DHA into myocardial membranes of rats from low dietary FO intake within human dietary range and quantitatively assessed the influence of dietary n-6 PUFA. With rats fed diets containing 0.16%–5% FO, equal to 0.12%–8.7% energy (%en) as eicosapentaenoic acid (EPA) and DHA (EPA+DHA), and either 1.5%en or 7.5%en n-6 PUFA (linoleic acid) for four weeks, dietary n-6:n-3 PUFA ratios ranged from 74 to 0.3. Myocardial DHA concentration increased in a log-linear fashion with a dietary threshold of 0.019%en as EPA+DHA and half maximal dietary [EPA+DHA] equal to 0.29%en (95% CI, 0.23–0.35). Dietary linoleic acid intake did not influence myocardial DHA. Myocardial membranes are sensitive to absolute dietary intake of long chain n-3 PUFA at low %en in the rat, equivalent to a human intake of one meal of fatty fish per week or less. The dietary ratio of n-6:n-3 PUFA has no influence on long chain n-3 PUFA cellular incorporation from dietary fish oil. |
topic |
myocardial membrane dietary lipid docosahexaenoic acid omega-3 fatty acid polyunsaturated fatty acid n-6:n-3 PUFA ratio |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520371066 |
work_keys_str_mv |
AT emilylslee lowdietaryfishoilthresholdformyocardialmembranen3pufaenrichmentindependentofn6pufaintakeinrats AT peterlmclennan lowdietaryfishoilthresholdformyocardialmembranen3pufaenrichmentindependentofn6pufaintakeinrats AT alicejowen lowdietaryfishoilthresholdformyocardialmembranen3pufaenrichmentindependentofn6pufaintakeinrats AT mandyltheiss lowdietaryfishoilthresholdformyocardialmembranen3pufaenrichmentindependentofn6pufaintakeinrats |
_version_ |
1721504528260399104 |