“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media

This article overviews the numerous immobilization methods available for various biocatalysts such as whole-cells, cell fragments, lysates or enzymes which do not require preliminary enzyme purification and introduces an advanced approach avoiding the costly and time consuming downstream processes r...

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Main Authors:	Evelin Sánta-Bell, Zsófia Molnár, Andrea Varga, Flóra Nagy, Gábor Hornyánszky, Csaba Paizs, Diána Balogh-Weiser, László Poppe
Format:	Article
Language:	English
Published:	MDPI AG 2019-11-01
Series:	Molecules
Subjects:	imac selective enzyme immobilization phenylalanine ammonia-lyase magnetic nanoparticles
Online Access:	https://www.mdpi.com/1420-3049/24/22/4146

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spelling	doaj-e723714cbb31483da8f6dd5860fdbad42020-11-25T01:32:38ZengMDPI AGMolecules1420-30492019-11-012422414610.3390/molecules24224146molecules24224146“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation MediaEvelin Sánta-Bell0Zsófia Molnár1Andrea Varga2Flóra Nagy3Gábor Hornyánszky4Csaba Paizs5Diána Balogh-Weiser6László Poppe7Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, 1111 Budapest, HungaryDepartment of Organic Chemistry and Technology, Budapest University of Technology and Economics, 1111 Budapest, HungaryBiocatalysis and Biotransformation Research Centre, Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University of Cluj-Napoca, 400028 Cluj-Napoca, RomaniaDepartment of Organic Chemistry and Technology, Budapest University of Technology and Economics, 1111 Budapest, HungaryDepartment of Organic Chemistry and Technology, Budapest University of Technology and Economics, 1111 Budapest, HungaryBiocatalysis and Biotransformation Research Centre, Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University of Cluj-Napoca, 400028 Cluj-Napoca, RomaniaDepartment of Organic Chemistry and Technology, Budapest University of Technology and Economics, 1111 Budapest, HungaryDepartment of Organic Chemistry and Technology, Budapest University of Technology and Economics, 1111 Budapest, HungaryThis article overviews the numerous immobilization methods available for various biocatalysts such as whole-cells, cell fragments, lysates or enzymes which do not require preliminary enzyme purification and introduces an advanced approach avoiding the costly and time consuming downstream processes required by immobilization of purified enzyme-based biocatalysts (such as enzyme purification by chromatographic methods and dialysis). Our approach is based on silica shell coated magnetic nanoparticles as solid carriers decorated with mixed functions having either coordinative binding ability (a metal ion complexed by a chelator anchored to the surface) or covalent bond-forming ability (an epoxide attached to the surface via a proper linker) enabling a single operation enrichment and immobilization of a recombinant phenylalanine ammonia-lyase from parsley fused to a polyhistidine affinity tag.https://www.mdpi.com/1420-3049/24/22/4146imacselective enzyme immobilizationphenylalanine ammonia-lyasemagnetic nanoparticles
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Evelin Sánta-Bell Zsófia Molnár Andrea Varga Flóra Nagy Gábor Hornyánszky Csaba Paizs Diána Balogh-Weiser László Poppe
spellingShingle	Evelin Sánta-Bell Zsófia Molnár Andrea Varga Flóra Nagy Gábor Hornyánszky Csaba Paizs Diána Balogh-Weiser László Poppe “Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media Molecules imac selective enzyme immobilization phenylalanine ammonia-lyase magnetic nanoparticles
author_facet	Evelin Sánta-Bell Zsófia Molnár Andrea Varga Flóra Nagy Gábor Hornyánszky Csaba Paizs Diána Balogh-Weiser László Poppe
author_sort	Evelin Sánta-Bell
title	“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media
title_short	“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media
title_full	“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media
title_fullStr	“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media
title_full_unstemmed	“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media
title_sort	“fishing and hunting”—selective immobilization of a recombinant phenylalanine ammonia-lyase from fermentation media
publisher	MDPI AG
series	Molecules
issn	1420-3049
publishDate	2019-11-01
description	This article overviews the numerous immobilization methods available for various biocatalysts such as whole-cells, cell fragments, lysates or enzymes which do not require preliminary enzyme purification and introduces an advanced approach avoiding the costly and time consuming downstream processes required by immobilization of purified enzyme-based biocatalysts (such as enzyme purification by chromatographic methods and dialysis). Our approach is based on silica shell coated magnetic nanoparticles as solid carriers decorated with mixed functions having either coordinative binding ability (a metal ion complexed by a chelator anchored to the surface) or covalent bond-forming ability (an epoxide attached to the surface via a proper linker) enabling a single operation enrichment and immobilization of a recombinant phenylalanine ammonia-lyase from parsley fused to a polyhistidine affinity tag.
topic	imac selective enzyme immobilization phenylalanine ammonia-lyase magnetic nanoparticles
url	https://www.mdpi.com/1420-3049/24/22/4146
work_keys_str_mv	AT evelinsantabell fishingandhuntingselectiveimmobilizationofarecombinantphenylalanineammonialyasefromfermentationmedia AT zsofiamolnar fishingandhuntingselectiveimmobilizationofarecombinantphenylalanineammonialyasefromfermentationmedia AT andreavarga fishingandhuntingselectiveimmobilizationofarecombinantphenylalanineammonialyasefromfermentationmedia AT floranagy fishingandhuntingselectiveimmobilizationofarecombinantphenylalanineammonialyasefromfermentationmedia AT gaborhornyanszky fishingandhuntingselectiveimmobilizationofarecombinantphenylalanineammonialyasefromfermentationmedia AT csabapaizs fishingandhuntingselectiveimmobilizationofarecombinantphenylalanineammonialyasefromfermentationmedia AT dianabaloghweiser fishingandhuntingselectiveimmobilizationofarecombinantphenylalanineammonialyasefromfermentationmedia AT laszlopoppe fishingandhuntingselectiveimmobilizationofarecombinantphenylalanineammonialyasefromfermentationmedia
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“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media

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