“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media
This article overviews the numerous immobilization methods available for various biocatalysts such as whole-cells, cell fragments, lysates or enzymes which do not require preliminary enzyme purification and introduces an advanced approach avoiding the costly and time consuming downstream processes r...
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doaj-e723714cbb31483da8f6dd5860fdbad42020-11-25T01:32:38ZengMDPI AGMolecules1420-30492019-11-012422414610.3390/molecules24224146molecules24224146“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation MediaEvelin Sánta-Bell0Zsófia Molnár1Andrea Varga2Flóra Nagy3Gábor Hornyánszky4Csaba Paizs5Diána Balogh-Weiser6László Poppe7Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, 1111 Budapest, HungaryDepartment of Organic Chemistry and Technology, Budapest University of Technology and Economics, 1111 Budapest, HungaryBiocatalysis and Biotransformation Research Centre, Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University of Cluj-Napoca, 400028 Cluj-Napoca, RomaniaDepartment of Organic Chemistry and Technology, Budapest University of Technology and Economics, 1111 Budapest, HungaryDepartment of Organic Chemistry and Technology, Budapest University of Technology and Economics, 1111 Budapest, HungaryBiocatalysis and Biotransformation Research Centre, Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University of Cluj-Napoca, 400028 Cluj-Napoca, RomaniaDepartment of Organic Chemistry and Technology, Budapest University of Technology and Economics, 1111 Budapest, HungaryDepartment of Organic Chemistry and Technology, Budapest University of Technology and Economics, 1111 Budapest, HungaryThis article overviews the numerous immobilization methods available for various biocatalysts such as whole-cells, cell fragments, lysates or enzymes which do not require preliminary enzyme purification and introduces an advanced approach avoiding the costly and time consuming downstream processes required by immobilization of purified enzyme-based biocatalysts (such as enzyme purification by chromatographic methods and dialysis). Our approach is based on silica shell coated magnetic nanoparticles as solid carriers decorated with mixed functions having either coordinative binding ability (a metal ion complexed by a chelator anchored to the surface) or covalent bond-forming ability (an epoxide attached to the surface via a proper linker) enabling a single operation enrichment and immobilization of a recombinant phenylalanine ammonia-lyase from parsley fused to a polyhistidine affinity tag.https://www.mdpi.com/1420-3049/24/22/4146imacselective enzyme immobilizationphenylalanine ammonia-lyasemagnetic nanoparticles |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Evelin Sánta-Bell Zsófia Molnár Andrea Varga Flóra Nagy Gábor Hornyánszky Csaba Paizs Diána Balogh-Weiser László Poppe |
spellingShingle |
Evelin Sánta-Bell Zsófia Molnár Andrea Varga Flóra Nagy Gábor Hornyánszky Csaba Paizs Diána Balogh-Weiser László Poppe “Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media Molecules imac selective enzyme immobilization phenylalanine ammonia-lyase magnetic nanoparticles |
author_facet |
Evelin Sánta-Bell Zsófia Molnár Andrea Varga Flóra Nagy Gábor Hornyánszky Csaba Paizs Diána Balogh-Weiser László Poppe |
author_sort |
Evelin Sánta-Bell |
title |
“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media |
title_short |
“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media |
title_full |
“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media |
title_fullStr |
“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media |
title_full_unstemmed |
“Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media |
title_sort |
“fishing and hunting”—selective immobilization of a recombinant phenylalanine ammonia-lyase from fermentation media |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2019-11-01 |
description |
This article overviews the numerous immobilization methods available for various biocatalysts such as whole-cells, cell fragments, lysates or enzymes which do not require preliminary enzyme purification and introduces an advanced approach avoiding the costly and time consuming downstream processes required by immobilization of purified enzyme-based biocatalysts (such as enzyme purification by chromatographic methods and dialysis). Our approach is based on silica shell coated magnetic nanoparticles as solid carriers decorated with mixed functions having either coordinative binding ability (a metal ion complexed by a chelator anchored to the surface) or covalent bond-forming ability (an epoxide attached to the surface via a proper linker) enabling a single operation enrichment and immobilization of a recombinant phenylalanine ammonia-lyase from parsley fused to a polyhistidine affinity tag. |
topic |
imac selective enzyme immobilization phenylalanine ammonia-lyase magnetic nanoparticles |
url |
https://www.mdpi.com/1420-3049/24/22/4146 |
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