LncRNA miR503HG Regulates the Drug Resistance of Recurrent Cervical Squamous Cell Carcinoma Cells by Regulating miR-155/Caspase-3
Shanshan Zhao, Mingxin Yu, Lei Wang Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Gynaecology, Shenyang 110042, Liaoning Province, People’s Republic of ChinaCorrespondence: Mingxin YuCancer Hospital of China Medical University, Liaoning Cancer H...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Dove Medical Press
2020-03-01
|
Series: | Cancer Management and Research |
Subjects: | |
Online Access: | https://www.dovepress.com/lncrna-mir503hg-regulates-the-drug-resistance-of-recurrent-cervical-sq-peer-reviewed-article-CMAR |
id |
doaj-e7261f02d16e418b9e2857ac4d772ba2 |
---|---|
record_format |
Article |
spelling |
doaj-e7261f02d16e418b9e2857ac4d772ba22020-11-25T02:56:00ZengDove Medical PressCancer Management and Research1179-13222020-03-01Volume 121579158552233LncRNA miR503HG Regulates the Drug Resistance of Recurrent Cervical Squamous Cell Carcinoma Cells by Regulating miR-155/Caspase-3Zhao SYu MWang LShanshan Zhao, Mingxin Yu, Lei Wang Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Gynaecology, Shenyang 110042, Liaoning Province, People’s Republic of ChinaCorrespondence: Mingxin YuCancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Gynaecology, No. 44 Xiaoheyan Road, Dadong District, Shenyang 110042, Liaoning Province, People’s Republic of ChinaTel +86 138 4033 2413Email yumingxin5066@aliyun.comIntroduction: The purpose of this study is to investigate the role of long non-coding RNA (lncRNA) miR503 Host Gene (miR503HG) in cervical squamous cell carcinoma (CSCC).Methods: Analysis of TCGA dataset revealed that expression levels of miR503HG in CSCC tissues were over 12 times lower than those in non-tumor tissues, indicating its involvement in CSCC.Results: In this study, we observed that levels of miR503HG in plasma were significantly lower in CSCC patients than in healthy participants. The cisplatin-based treatment further downregulated miR503HG in both patients and CSCC cells. MiR503HG overexpression in CSCC cells led to the suppression of miR-155 and elevation of Caspase-3, acting as the downstream target of miR-155. Cell apoptosis analysis showed that miR503HG and Caspase-3 overexpression led to an increased cell apoptosis rate under Cisplatin treatment. MiR-155 played the opposite role and attenuated the functions of Caspase-3 and miR503HG overexpression.Conclusion: Therefore, miR503HG may regulate the drug resistance of CSCC cells by regulating mir-155/Caspase-3.Keywords: cervical squamous cell carcinoma, miR503HG, miR-155, Caspase-3, apoptosishttps://www.dovepress.com/lncrna-mir503hg-regulates-the-drug-resistance-of-recurrent-cervical-sq-peer-reviewed-article-CMARcervical squamous cell carcinomamir503hgmir-155caspase-3apoptosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhao S Yu M Wang L |
spellingShingle |
Zhao S Yu M Wang L LncRNA miR503HG Regulates the Drug Resistance of Recurrent Cervical Squamous Cell Carcinoma Cells by Regulating miR-155/Caspase-3 Cancer Management and Research cervical squamous cell carcinoma mir503hg mir-155 caspase-3 apoptosis |
author_facet |
Zhao S Yu M Wang L |
author_sort |
Zhao S |
title |
LncRNA miR503HG Regulates the Drug Resistance of Recurrent Cervical Squamous Cell Carcinoma Cells by Regulating miR-155/Caspase-3 |
title_short |
LncRNA miR503HG Regulates the Drug Resistance of Recurrent Cervical Squamous Cell Carcinoma Cells by Regulating miR-155/Caspase-3 |
title_full |
LncRNA miR503HG Regulates the Drug Resistance of Recurrent Cervical Squamous Cell Carcinoma Cells by Regulating miR-155/Caspase-3 |
title_fullStr |
LncRNA miR503HG Regulates the Drug Resistance of Recurrent Cervical Squamous Cell Carcinoma Cells by Regulating miR-155/Caspase-3 |
title_full_unstemmed |
LncRNA miR503HG Regulates the Drug Resistance of Recurrent Cervical Squamous Cell Carcinoma Cells by Regulating miR-155/Caspase-3 |
title_sort |
lncrna mir503hg regulates the drug resistance of recurrent cervical squamous cell carcinoma cells by regulating mir-155/caspase-3 |
publisher |
Dove Medical Press |
series |
Cancer Management and Research |
issn |
1179-1322 |
publishDate |
2020-03-01 |
description |
Shanshan Zhao, Mingxin Yu, Lei Wang Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Gynaecology, Shenyang 110042, Liaoning Province, People’s Republic of ChinaCorrespondence: Mingxin YuCancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Gynaecology, No. 44 Xiaoheyan Road, Dadong District, Shenyang 110042, Liaoning Province, People’s Republic of ChinaTel +86 138 4033 2413Email yumingxin5066@aliyun.comIntroduction: The purpose of this study is to investigate the role of long non-coding RNA (lncRNA) miR503 Host Gene (miR503HG) in cervical squamous cell carcinoma (CSCC).Methods: Analysis of TCGA dataset revealed that expression levels of miR503HG in CSCC tissues were over 12 times lower than those in non-tumor tissues, indicating its involvement in CSCC.Results: In this study, we observed that levels of miR503HG in plasma were significantly lower in CSCC patients than in healthy participants. The cisplatin-based treatment further downregulated miR503HG in both patients and CSCC cells. MiR503HG overexpression in CSCC cells led to the suppression of miR-155 and elevation of Caspase-3, acting as the downstream target of miR-155. Cell apoptosis analysis showed that miR503HG and Caspase-3 overexpression led to an increased cell apoptosis rate under Cisplatin treatment. MiR-155 played the opposite role and attenuated the functions of Caspase-3 and miR503HG overexpression.Conclusion: Therefore, miR503HG may regulate the drug resistance of CSCC cells by regulating mir-155/Caspase-3.Keywords: cervical squamous cell carcinoma, miR503HG, miR-155, Caspase-3, apoptosis |
topic |
cervical squamous cell carcinoma mir503hg mir-155 caspase-3 apoptosis |
url |
https://www.dovepress.com/lncrna-mir503hg-regulates-the-drug-resistance-of-recurrent-cervical-sq-peer-reviewed-article-CMAR |
work_keys_str_mv |
AT zhaos lncrnamir503hgregulatesthedrugresistanceofrecurrentcervicalsquamouscellcarcinomacellsbyregulatingmir155caspase3 AT yum lncrnamir503hgregulatesthedrugresistanceofrecurrentcervicalsquamouscellcarcinomacellsbyregulatingmir155caspase3 AT wangl lncrnamir503hgregulatesthedrugresistanceofrecurrentcervicalsquamouscellcarcinomacellsbyregulatingmir155caspase3 |
_version_ |
1724714848616448000 |