The antibiotic erythromycin did not affect micronucleus frequency in human PHA-stimulated lymphocytes

The aim of the present study was to investigate the ability of the antibiotic erythromycin to induce micro­nucleus (MN) frequency and the cytokinesis block proliferation index (CBPI) in human peripheral blood lymphocytes in vitro using the cytokinesis-block micronucleus test (CBMN). Cell cultures we...

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Main Authors: Grujičić D., Stošić Ivana, Milošević-Đorđević Olivera
Format: Article
Language:English
Published: University of Belgrade, University of Novi Sad 2009-01-01
Series:Archives of Biological Sciences
Subjects:
Online Access:http://www.doiserbia.nb.rs/img/doi/0354-4664/2009/0354-46640902179G.pdf
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spelling doaj-e7428ffeea8c443c884e4a4ed28a317e2020-11-25T00:25:42ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46642009-01-0161217918510.2298/ABS0902179GThe antibiotic erythromycin did not affect micronucleus frequency in human PHA-stimulated lymphocytesGrujičić D.Stošić IvanaMilošević-Đorđević OliveraThe aim of the present study was to investigate the ability of the antibiotic erythromycin to induce micro­nucleus (MN) frequency and the cytokinesis block proliferation index (CBPI) in human peripheral blood lymphocytes in vitro using the cytokinesis-block micronucleus test (CBMN). Cell cultures were treated with five different concentrations of erythromycin ranging from 0.68 x 10-4 to 5.45 x 10-4 M. The positive control cells were treated with a known mutagen, mitomycin C (MMC), in a concentration of 1.6 x 10-6 M. None of the tested concentrations of erythromycin significantly changed the average MN frequency or CBPI in PHA-stimulated lymphocytes (p > 0.05). http://www.doiserbia.nb.rs/img/doi/0354-4664/2009/0354-46640902179G.pdferythromycinhuman peripheral blood lymphocytesin vitro effectmicronucleus test
collection DOAJ
language English
format Article
sources DOAJ
author Grujičić D.
Stošić Ivana
Milošević-Đorđević Olivera
spellingShingle Grujičić D.
Stošić Ivana
Milošević-Đorđević Olivera
The antibiotic erythromycin did not affect micronucleus frequency in human PHA-stimulated lymphocytes
Archives of Biological Sciences
erythromycin
human peripheral blood lymphocytes
in vitro effect
micronucleus test
author_facet Grujičić D.
Stošić Ivana
Milošević-Đorđević Olivera
author_sort Grujičić D.
title The antibiotic erythromycin did not affect micronucleus frequency in human PHA-stimulated lymphocytes
title_short The antibiotic erythromycin did not affect micronucleus frequency in human PHA-stimulated lymphocytes
title_full The antibiotic erythromycin did not affect micronucleus frequency in human PHA-stimulated lymphocytes
title_fullStr The antibiotic erythromycin did not affect micronucleus frequency in human PHA-stimulated lymphocytes
title_full_unstemmed The antibiotic erythromycin did not affect micronucleus frequency in human PHA-stimulated lymphocytes
title_sort antibiotic erythromycin did not affect micronucleus frequency in human pha-stimulated lymphocytes
publisher University of Belgrade, University of Novi Sad
series Archives of Biological Sciences
issn 0354-4664
publishDate 2009-01-01
description The aim of the present study was to investigate the ability of the antibiotic erythromycin to induce micro­nucleus (MN) frequency and the cytokinesis block proliferation index (CBPI) in human peripheral blood lymphocytes in vitro using the cytokinesis-block micronucleus test (CBMN). Cell cultures were treated with five different concentrations of erythromycin ranging from 0.68 x 10-4 to 5.45 x 10-4 M. The positive control cells were treated with a known mutagen, mitomycin C (MMC), in a concentration of 1.6 x 10-6 M. None of the tested concentrations of erythromycin significantly changed the average MN frequency or CBPI in PHA-stimulated lymphocytes (p > 0.05).
topic erythromycin
human peripheral blood lymphocytes
in vitro effect
micronucleus test
url http://www.doiserbia.nb.rs/img/doi/0354-4664/2009/0354-46640902179G.pdf
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