TBX3 Knockdown Decreases Reprogramming Efficiency of Human Cells

TBX3 Knockdown Decreases Reprogramming Efficiency of Human Cells

TBX3 is a member of the T-box transcription factor family and is involved in the core pluripotency network. Despite this role in the pluripotency network, its contribution to the reprogramming process during the generation of human induced pluripotent stem cells remains elusive. In this respect, we...

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Main Authors: Moritz Klingenstein, Stefanie Raab, Kevin Achberger, Alexander Kleger, Stefan Liebau, Leonhard Linta
Format: Article
Language:English
Published: Hindawi Limited 2016-01-01
Series:Stem Cells International
Online Access:http://dx.doi.org/10.1155/2016/6759343
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spelling doaj-e747b74e6cbc4cac94fe9deeeb549a922020-11-24T22:39:00ZengHindawi LimitedStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/67593436759343TBX3 Knockdown Decreases Reprogramming Efficiency of Human CellsMoritz Klingenstein0Stefanie Raab1Kevin Achberger2Alexander Kleger3Stefan Liebau4Leonhard Linta5Institute of Neuroanatomy, Eberhard Karls University Tübingen, 72074 Tübingen, GermanyInstitute of Neuroanatomy, Eberhard Karls University Tübingen, 72074 Tübingen, GermanyInstitute of Neuroanatomy, Eberhard Karls University Tübingen, 72074 Tübingen, GermanyDepartment of Internal Medicine I, Ulm University, 89081 Ulm, GermanyInstitute of Neuroanatomy, Eberhard Karls University Tübingen, 72074 Tübingen, GermanyInstitute of Neuroanatomy, Eberhard Karls University Tübingen, 72074 Tübingen, GermanyTBX3 is a member of the T-box transcription factor family and is involved in the core pluripotency network. Despite this role in the pluripotency network, its contribution to the reprogramming process during the generation of human induced pluripotent stem cells remains elusive. In this respect, we performed reprogramming experiments applying TBX3 knockdown in human fibroblasts and keratinocytes. Knockdown of TBX3 in both somatic cell types decreased the reprogramming efficiencies in comparison to control cells but with unchanged reprogramming kinetics. The resulting iPSCs were indistinguishable from control cells and displayed a normal in vitro differentiation capacity by generating cells of all three germ layers comparable to the controls.http://dx.doi.org/10.1155/2016/6759343
collection DOAJ
language English
format Article
sources DOAJ
author Moritz Klingenstein
Stefanie Raab
Kevin Achberger
Alexander Kleger
Stefan Liebau
Leonhard Linta
spellingShingle Moritz Klingenstein
Stefanie Raab
Kevin Achberger
Alexander Kleger
Stefan Liebau
Leonhard Linta
TBX3 Knockdown Decreases Reprogramming Efficiency of Human Cells
Stem Cells International
author_facet Moritz Klingenstein
Stefanie Raab
Kevin Achberger
Alexander Kleger
Stefan Liebau
Leonhard Linta
author_sort Moritz Klingenstein
title TBX3 Knockdown Decreases Reprogramming Efficiency of Human Cells
title_short TBX3 Knockdown Decreases Reprogramming Efficiency of Human Cells
title_full TBX3 Knockdown Decreases Reprogramming Efficiency of Human Cells
title_fullStr TBX3 Knockdown Decreases Reprogramming Efficiency of Human Cells
title_full_unstemmed TBX3 Knockdown Decreases Reprogramming Efficiency of Human Cells
title_sort tbx3 knockdown decreases reprogramming efficiency of human cells
publisher Hindawi Limited
series Stem Cells International
issn 1687-966X
1687-9678
publishDate 2016-01-01
description TBX3 is a member of the T-box transcription factor family and is involved in the core pluripotency network. Despite this role in the pluripotency network, its contribution to the reprogramming process during the generation of human induced pluripotent stem cells remains elusive. In this respect, we performed reprogramming experiments applying TBX3 knockdown in human fibroblasts and keratinocytes. Knockdown of TBX3 in both somatic cell types decreased the reprogramming efficiencies in comparison to control cells but with unchanged reprogramming kinetics. The resulting iPSCs were indistinguishable from control cells and displayed a normal in vitro differentiation capacity by generating cells of all three germ layers comparable to the controls.
url http://dx.doi.org/10.1155/2016/6759343
work_keys_str_mv AT moritzklingenstein tbx3knockdowndecreasesreprogrammingefficiencyofhumancells
AT stefanieraab tbx3knockdowndecreasesreprogrammingefficiencyofhumancells
AT kevinachberger tbx3knockdowndecreasesreprogrammingefficiencyofhumancells
AT alexanderkleger tbx3knockdowndecreasesreprogrammingefficiencyofhumancells
AT stefanliebau tbx3knockdowndecreasesreprogrammingefficiencyofhumancells
AT leonhardlinta tbx3knockdowndecreasesreprogrammingefficiencyofhumancells
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