RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold
Cold exposure greatly alters brown adipose tissue (BAT) gene expression and metabolism to increase thermogenic capacity. Here, we used RNA sequencing and mass-spectrometry-based lipidomics to provide a comprehensive resource describing the molecular signature of cold adaptation at the level of the t...
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doaj-e749a7d550da4a318785f7f55d4b5e072020-11-25T01:28:16ZengElsevierCell Reports2211-12472015-12-011392000201310.1016/j.celrep.2015.10.069RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to ColdAnn-Britt Marcher0Anne Loft1Ronni Nielsen2Terhi Vihervaara3Jesper Grud Skat Madsen4Marko Sysi-Aho5Kim Ekroos6Susanne Mandrup7Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, DenmarkDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, DenmarkDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, DenmarkZora Biosciences Oy, Biologinkuja 1, 02150 Espoo, FinlandDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, DenmarkZora Biosciences Oy, Biologinkuja 1, 02150 Espoo, FinlandZora Biosciences Oy, Biologinkuja 1, 02150 Espoo, FinlandDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, DenmarkCold exposure greatly alters brown adipose tissue (BAT) gene expression and metabolism to increase thermogenic capacity. Here, we used RNA sequencing and mass-spectrometry-based lipidomics to provide a comprehensive resource describing the molecular signature of cold adaptation at the level of the transcriptome and lipidome. We show that short-term (3-day) cold exposure leads to a robust increase in expression of several brown adipocyte genes related to thermogenesis as well as the gene encoding the hormone irisin. However, pathway analysis shows that the most significantly induced genes are those involved in glycerophospholipid synthesis and fatty acid elongation. This is accompanied by significant changes in the acyl chain composition of triacylglycerols (TAGs) as well as subspecies-selective changes of acyl chains in glycerophospholipids. These results indicate that cold adaptation of BAT is associated with significant and highly species-selective remodeling of both TAGs and glycerophospholipids.http://www.sciencedirect.com/science/article/pii/S2211124715012589 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ann-Britt Marcher Anne Loft Ronni Nielsen Terhi Vihervaara Jesper Grud Skat Madsen Marko Sysi-Aho Kim Ekroos Susanne Mandrup |
spellingShingle |
Ann-Britt Marcher Anne Loft Ronni Nielsen Terhi Vihervaara Jesper Grud Skat Madsen Marko Sysi-Aho Kim Ekroos Susanne Mandrup RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold Cell Reports |
author_facet |
Ann-Britt Marcher Anne Loft Ronni Nielsen Terhi Vihervaara Jesper Grud Skat Madsen Marko Sysi-Aho Kim Ekroos Susanne Mandrup |
author_sort |
Ann-Britt Marcher |
title |
RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold |
title_short |
RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold |
title_full |
RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold |
title_fullStr |
RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold |
title_full_unstemmed |
RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold |
title_sort |
rna-seq and mass-spectrometry-based lipidomics reveal extensive changes of glycerolipid pathways in brown adipose tissue in response to cold |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2015-12-01 |
description |
Cold exposure greatly alters brown adipose tissue (BAT) gene expression and metabolism to increase thermogenic capacity. Here, we used RNA sequencing and mass-spectrometry-based lipidomics to provide a comprehensive resource describing the molecular signature of cold adaptation at the level of the transcriptome and lipidome. We show that short-term (3-day) cold exposure leads to a robust increase in expression of several brown adipocyte genes related to thermogenesis as well as the gene encoding the hormone irisin. However, pathway analysis shows that the most significantly induced genes are those involved in glycerophospholipid synthesis and fatty acid elongation. This is accompanied by significant changes in the acyl chain composition of triacylglycerols (TAGs) as well as subspecies-selective changes of acyl chains in glycerophospholipids. These results indicate that cold adaptation of BAT is associated with significant and highly species-selective remodeling of both TAGs and glycerophospholipids. |
url |
http://www.sciencedirect.com/science/article/pii/S2211124715012589 |
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