Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate
Kyu Hwan Shim,1 John Hulme,1 Eun Ho Maeng,2 Meyoung-Kon Kim,3 Seong Soo A An1 1Department of Bionano Technology, Gachon Medical Research Institute, Gachon University, Sungnam-si, 2Department of Analysis, KTR, Kimpo, Gyeonggi-do, 3Department of Biochemistry and Molecular Biology, Korea University Me...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Dove Medical Press
2014-12-01
|
Series: | International Journal of Nanomedicine |
Online Access: | http://www.dovepress.com/analysis-of-sio2-nanoparticles-binding-proteins-in-rat-blood-and-brain-peer-reviewed-article-IJN |
id |
doaj-e750c554aa2349e69787aed117ce0623 |
---|---|
record_format |
Article |
spelling |
doaj-e750c554aa2349e69787aed117ce06232020-11-24T20:41:44ZengDove Medical PressInternational Journal of Nanomedicine1178-20132014-12-012014Supplement 220721519590Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenateShim KHHulme JMaeng EHKim MKAn SSA Kyu Hwan Shim,1 John Hulme,1 Eun Ho Maeng,2 Meyoung-Kon Kim,3 Seong Soo A An1 1Department of Bionano Technology, Gachon Medical Research Institute, Gachon University, Sungnam-si, 2Department of Analysis, KTR, Kimpo, Gyeonggi-do, 3Department of Biochemistry and Molecular Biology, Korea University Medical School and College, Seoul, South Korea Abstract: A multitude of nanoparticles, such as titanium oxide (TiO2), zinc oxide, aluminum oxide, gold oxide, silver oxide, iron oxide, and silica oxide, are found in many chemical, cosmetic, pharmaceutical, and electronic products. Recently, SiO2 nanoparticles were shown to have an inert toxicity profile and no association with an irreversible toxicological change in animal models. Hence, exposure to SiO2 nanoparticles is on the increase. SiO2 nanoparticles are routinely used in numerous materials, from strengthening filler for concrete and other construction composites, to nontoxic platforms for biomedical application, such as drug delivery and theragnostics. On the other hand, recent in vitro experiments indicated that SiO2 nanoparticles were cytotoxic. Therefore, we investigated these nanoparticles to identify potentially toxic pathways by analyzing the adsorbed protein corona on the surface of SiO2 nanoparticles in the blood and brain of the rat. Four types of SiO2 nanoparticles were chosen for investigation, and the protein corona of each type was analyzed using liquid chromatography-tandem mass spectrometry technology. In total, 115 and 48 plasma proteins from the rat were identified as being bound to negatively charged 20 nm and 100 nm SiO2 nanoparticles, respectively, and 50 and 36 proteins were found for 20 nm and 100 nm arginine-coated SiO2 nanoparticles, respectively. Higher numbers of proteins were adsorbed onto the 20 nm sized SiO2 nanoparticles than onto the 100 nm sized nanoparticles regardless of charge. When proteins were compared between the two charges, higher numbers of proteins were found for arginine-coated positively charged SiO2 nanoparticles than for the negatively charged nanoparticles. The proteins identified as bound in the corona from SiO2 nanoparticles were further analyzed with ClueGO, a Cytoscape plugin used in protein ontology and for identifying biological interaction pathways. Proteins bound on the surface of nanoparticles may affect functional and conformational properties and distributions in complicated biological processes. Keywords: silica, nanoparticles, protein corona, plasma, brain homogenate, nanotoxicityhttp://www.dovepress.com/analysis-of-sio2-nanoparticles-binding-proteins-in-rat-blood-and-brain-peer-reviewed-article-IJN |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shim KH Hulme J Maeng EH Kim MK An SSA |
spellingShingle |
Shim KH Hulme J Maeng EH Kim MK An SSA Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate International Journal of Nanomedicine |
author_facet |
Shim KH Hulme J Maeng EH Kim MK An SSA |
author_sort |
Shim KH |
title |
Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate |
title_short |
Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate |
title_full |
Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate |
title_fullStr |
Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate |
title_full_unstemmed |
Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate |
title_sort |
analysis of sio2 nanoparticles binding proteins in rat blood and brain homogenate |
publisher |
Dove Medical Press |
series |
International Journal of Nanomedicine |
issn |
1178-2013 |
publishDate |
2014-12-01 |
description |
Kyu Hwan Shim,1 John Hulme,1 Eun Ho Maeng,2 Meyoung-Kon Kim,3 Seong Soo A An1 1Department of Bionano Technology, Gachon Medical Research Institute, Gachon University, Sungnam-si, 2Department of Analysis, KTR, Kimpo, Gyeonggi-do, 3Department of Biochemistry and Molecular Biology, Korea University Medical School and College, Seoul, South Korea Abstract: A multitude of nanoparticles, such as titanium oxide (TiO2), zinc oxide, aluminum oxide, gold oxide, silver oxide, iron oxide, and silica oxide, are found in many chemical, cosmetic, pharmaceutical, and electronic products. Recently, SiO2 nanoparticles were shown to have an inert toxicity profile and no association with an irreversible toxicological change in animal models. Hence, exposure to SiO2 nanoparticles is on the increase. SiO2 nanoparticles are routinely used in numerous materials, from strengthening filler for concrete and other construction composites, to nontoxic platforms for biomedical application, such as drug delivery and theragnostics. On the other hand, recent in vitro experiments indicated that SiO2 nanoparticles were cytotoxic. Therefore, we investigated these nanoparticles to identify potentially toxic pathways by analyzing the adsorbed protein corona on the surface of SiO2 nanoparticles in the blood and brain of the rat. Four types of SiO2 nanoparticles were chosen for investigation, and the protein corona of each type was analyzed using liquid chromatography-tandem mass spectrometry technology. In total, 115 and 48 plasma proteins from the rat were identified as being bound to negatively charged 20 nm and 100 nm SiO2 nanoparticles, respectively, and 50 and 36 proteins were found for 20 nm and 100 nm arginine-coated SiO2 nanoparticles, respectively. Higher numbers of proteins were adsorbed onto the 20 nm sized SiO2 nanoparticles than onto the 100 nm sized nanoparticles regardless of charge. When proteins were compared between the two charges, higher numbers of proteins were found for arginine-coated positively charged SiO2 nanoparticles than for the negatively charged nanoparticles. The proteins identified as bound in the corona from SiO2 nanoparticles were further analyzed with ClueGO, a Cytoscape plugin used in protein ontology and for identifying biological interaction pathways. Proteins bound on the surface of nanoparticles may affect functional and conformational properties and distributions in complicated biological processes. Keywords: silica, nanoparticles, protein corona, plasma, brain homogenate, nanotoxicity |
url |
http://www.dovepress.com/analysis-of-sio2-nanoparticles-binding-proteins-in-rat-blood-and-brain-peer-reviewed-article-IJN |
work_keys_str_mv |
AT shimkh analysisofsio2nanoparticlesbindingproteinsinratbloodandbrainhomogenate AT hulmej analysisofsio2nanoparticlesbindingproteinsinratbloodandbrainhomogenate AT maengeh analysisofsio2nanoparticlesbindingproteinsinratbloodandbrainhomogenate AT kimmk analysisofsio2nanoparticlesbindingproteinsinratbloodandbrainhomogenate AT anssa analysisofsio2nanoparticlesbindingproteinsinratbloodandbrainhomogenate |
_version_ |
1716824013262553088 |